Posted by admin on Mar 31, 2021 in |
A new stability indicating Ultra-Fast Liquid Chromatography (UFLC) method was validated for the simultaneous estimation of Metformin hydrochloride (MET) and Vildagliptin (VILDA) in bulk and tablet dosage form as per the International Council for Harmonization guidelines. Chromatography developed on Shimatzu UPLC, X-Bridge C18 column equipped with PDA detector. Mobile phase composed of phosphate buffer (25 mM) pH 3.0: acetonitrile: methanol (85:13:2). Sodium 1-octanesulfonate monohydrate (0.25 g/L) added in aqueous buffer. The effluent was passing through the column 1.0 ml/min at 40 ºC. The retention time of MET and VILDA were found to be 2.964 min and 6.358 min. The specificity study was determined by the addition of known impurities 1-cynoguanidine and vildagliptin amide of MET and VILDA, respectively. The forced degradation studies indicated that MET was stable while VILDA was susceptible to alkaline hydrolysis and peroxide oxidation. The degradation product peaks were well resolved from the MET and VILDA peaks. Purity angle and purity threshold of the MET and VILDA peak showed spectral homogeneity over the entire peak region...
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Posted by admin on Mar 31, 2021 in |
Now a day’s interest towards natural and has been growing due to the unhealthy consequences of chemicals in the health industry; though, herbal substances possess several quality control and confirm pharmacological action issues. This present study was designed to determine the effects of Salix alba L. methanolic extract (MESAL) for its antioxidant and anti-inflammatory activity in rat models. Further to establish and validate a sensitive, fast and reproducible high performance thin layer chromatographic (HPTLC) method of two biomarker compounds Salicin and Ferulic acid from MESAL. The anti-inflammatory activity was studied by the carrageenan induced rat paw oedema method while DPPH free radical scavenging ability was utilized to determine the antioxidant activity. Additionally, the separation was performed by HPTLC with quantification of markers (Salicin and Ferulic acid). Among the different combinations of mobile phases used, best separation was achieved in Toluene: Ethyl acetate: Methanol: Formic Acid (5:3:1:1v/v/v/v). The MESAL exhibited antioxidant activity with a maximal inhibitory concentration (IC50) value of 400 µg/ml and exerted anti-inflammatory activity, wherein 70% protection was...
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Posted by admin on Mar 31, 2021 in |
A simple UV spectrophotometric method using the first order derivative technique was developed for the simultaneous estimation of tacrolimus and berberine hydrochloride in nanoparticles. The tacrolimus and berberine hydrochloride stock solutions were prepared methanol and scanned in UV- region for first-order derivative spectrum. The zero-crossing point for tacrolimus and berberine hydrochloride was 369 nm and 263 nm, respectively. Linearity was established over the concentration range of 0.5-2.5 μg/ml and 5-25 μg/ml for tacrolimus and berberine hydrochloride with correlation coefficient (r2) value 0.9933 and 0.9796respectively. The method was validated according to ICH guidelines for validation parameters like accuracy, precision, the limit of detection, and the limit of quantification. They found to be within limits. The method was successfully applied for the quantitative estimation of tacrolimus and berberine hydrochloride in...
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Posted by admin on Mar 31, 2021 in |
Background: The entire world is battling the coronavirus disease (COVID-19) that potentially leads to pneumonia, Acute Respiratory Distress Syndrome (ARDS), multi-organ failure, often resulting in death. Therefore, the utmost challenge from a medical perspective is to improve lung function before the patient requires intubation and critical care. In the same light, the use of low dose radiation therapy (LDRT) that has been used to treat various non-malignant inflammatory conditions can be exploited as a promising approach to combat ARDS in COVID-19 patients. LDRT is a century-old popular treatment of viral pneumonia that has been reported to reduce inflammation and prevent the cytokine storm, thus mitigating the severity of pneumonitis. Given the outcomes of pilot clinical studies, the administration of LDRT should be kept as an option in clinical situations where benefits outweigh the risks. Therefore, the present communication highlights the anti-inflammatory role of LDRT so as to revisit its prospective role in COVID-19 pneumonia treatment. Conclusions: In the COVID-19 pandemic, LDRT appears as a cost-efficient, potent, non-invasive, anti-inflammatory treatment...
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Posted by admin on Mar 31, 2021 in |
AIM: To see the appraisement of drug utilization pattern of antimicrobials in the general medicine department of a tertiary care teaching hospital. Methodology: A prospective observational, analytical study was done on patients admitted in general medicine of Maharaja Institute of Medical Sciences, Vizianagaram, Andhra Pradesh, India information regarding age, gender, diagnosis, patients present/past medical history, treatment, drug interactions were recorded in a standard questionnaire(case report form). The drug utilization process was evaluated using quality indicators of drug use recommendations by WHO. PDD of drugs and maximally used antimicrobials were analyzed. Results: A total of 250 patients were included after excluding missing data. Out of 250 patients, 123 (49.2%) were male, and 127 (50.8%) were female, 183 (65.12%) was bacterial, 74 (26.33%) were viral, 22 (7.82%) were protozoal, 2 (0.7%) were fungal, and the p-value was 0.0213, cephalosporins were most prescribed antimicrobial (27.72%), and anti-helminthics were least (0.33%), and p-value was found to be 0.0016. Out of 18 UTI cases, 3 were male, and 15 were female, and the p-value...
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