Posted by admin on Mar 31, 2025 in |
The utility of herbal medicines incresed for curative purposes in the past few years. They serve as a curative alternative for a significant section of the worldwide community. Nonetheless, assessing the reliability of such facilities continues to be challenging in guaranteeing their safe use. Acute and subacute toxicity studies were conducted in a rat model. The cytotoxicity assay and AO/PI staining of HALEPR was carried out in an endometrial cell. Acute toxicity was evaluated in female Charles Foster rats over 14 days, revealing no mortality or severe adverse effects, suggesting a Lethal Dose 50 (LD50) above 2000 mg/kg. Subacute toxicity was assessed over 28 days with no significant changes (P < 0.05) in body weight, feed consumption, water intake, or biochemical parameters. Histological examination of organ tissues indicated no notable differences between treated and control groups. The antiproliferative activity of HALEPR was investigated using primary endometrial cell cultures. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay demonstrated a significant apoptotic effect with an IC50 of 41.46 µg/mL. This result was further confirmed...
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Posted by admin on Mar 31, 2025 in |
Background: This study presents the extraction, fractionation, formulation, and evaluation of transdermal polyherbal films containing Curcumin and Capsaicin for potential anti-inflammatory use. Capsicum annuum extract underwent ethanol-based extraction and fractionation, isolating alkaloids for further study. Results: Phytochemical screening revealed the presence of alkaloids, flavonoids, polyphenols, and terpenoids in both Curcuma longa and Capsicum annuum extracts. Chemical fractionation was done for Capsicum annuum extract, Dichloromethane fraction given the best results for alkaloids. Compatibility studies employing FTIR and DSC demonstrated the compatibility of Curcumin and Capsaicin with excipients, crucial for film formulation. In-silico molecular docking studies elucidated interactions between Capsaicin/Curcumin and Cox-2 protein, hinting at potential anti-inflammatory activity. The films were formulated using chitosan and solid dispersions of Curcuminoids/Capsaicinoids with Eudragit® EPO. Evaluation encompassed various parameters like weight variation, thickness, folding endurance, moisture content, water uptake, drug content, and in-vitro drug release. Further assessment involved a Carrageenan-induced paw edema model in rats, comparing different film formulations with a standard Diclofenac patch for their anti-inflammatory efficacy. Conclusion: This comprehensive approach involving extraction,...
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Posted by admin on Mar 31, 2025 in |
Rheumatoid arthritis (RA) is a chronic inflammation disorder that primarily affects the joints and leads to the progressive destruction of joints, synovial hyperplasia bone, and cartilage degeneration. Globally 1% of the world population was affected by rheumatoid arthritis. The present study focused to identify the bioactive phytocompounds of polyherbal formulation- Erandadi Kwatham and to determine its potential in inhibiting the targets TNF-α and Hk-II, which are associated with rheumatoid arthritis. The 3D structure of the phytocompounds were retrieved and analyzed for its physicochemical properties, toxicity, and drug-likeness were determined using the Qikprop module in maestro Schrodinger software. Among, only six compounds satisfied the ADME properties and followed drug-likeness. Further the phytocompounds triethyl citrate (6506), butanedioic acid, hydroxyl diethyl ester (24197), L-Valine, ethyl ester (87182), phenylacetic acid (97942), 7-trihydroxy-1-methyl – 8 – methylene-1, 4a-lactone-10-methyl (539615), and pectolinarigenin (5320438), were analyzed using molecular docking studies against the protein targets, TNF-α and Hk-II. The compounds triethyl citrate and pectolinarigenin were observed with significant binding efficiency of -3.47 and -5.88 kcal/mol against the...
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Posted by admin on Mar 31, 2025 in |
Psychogenic non-epileptic seizures (PNES), which intently look like epileptic seizures (ES), are regularly set off by mental trouble and address the most pervasive type of change issue experienced in clinical practice. Different states of being can both encourage and support PNES episodes. PNES episodes. Epilepsy, a common neurological disorder, imposes significant emotional and physical burdens, frequently resulting in elevated levels of anxiety and depression. This case report details the clinical course of a 25-year-old female diagnosed with PNES. The patient background of childhood trauma, early marriage, and depression likely predisposed her to the development of PNES upon diagnosing with epilepsy, characterized by seizure and depression, the patient experienced increased anxiety, irritability, loss of consciousness, and required hospitalization. Her treatment regimen of intravenous administration of Injection Levetiracetam 500 mg and oral olanzapine 2.5 mg to her seizures persisted. On evaluation of electroencephalography EEG and CT scan indicated the epilepsy and Panes. Although the surgical intervention was unnecessary, the management of epilepsy with ongoing pharmacotherapy significantly reduced her PNES episodes. This...
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Posted by admin on Mar 31, 2025 in |
Oral candidiasis (OC) is a common fungal infection characterized by white patches on the oral mucosa, often triggered by factors like immunosuppression and medication use. It can affect anyone, regardless of age or background. The formulation of mucoadhesive gels for the treatment of oral candidiasis is a promising area of research. The gel enhances the residence time of the drug at the site of infection, thereby improving therapeutic efficacy. This study aimed to develop a mucoadhesive gel for oral candidiasis treatment using an inclusion complex of itraconazole and beta-cyclodextrin. Different concentrations of mucoadhesive polymers (Carbopol 940) and absorption enhancers (Poloxamer 188 and propylene glycol) were used. The pH of the gels ranged from 6.5 to 7.2. The viscosity varied between 300 and 600 cps. The gelation temperature was 30°C to 35°C. Spreadability ranged from 4.7 to 4.83 cm. The drug content was found to be 90% to 95%. Mucoadhesive force ranged from 3549 to 3917 dyne/cm2. Drug release lasted 6 hours, with 95 to 99% cumulative release. In-vitro antifungal...
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