Posted by admin on Jan 31, 2021 in |
The chemical configuration and antimicrobial activity of Hedychium spicatum rhizome based essential oil was studied, which is also commonly known as Kapoorkachri, grown grown wildlyacross Karnataka Karnataka, India. Thirty-eight compounds were detected from the rhizome based essential oil by GCMS. The main compound was characterized as Ethyl p-methoxy-cinnamate (50.1%), Ethyl cinnamate (26.22%), and Eucalyptol (5. 68%). The anti-bacterial activity was performed by the Broth dilution method. Oil-DMSO solution in seven dilutions was developed starting from 100%, 50%, 25%, 12.5%, 6.25%, 3.12% and 1.56% against five selected human pathogenic organisms which comprised of Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. Anti-microbial activity against these pathogens were found to be 25%,25%,25%, 50% and 1.56% respectively. The oil showed positive inhibitory effects on organisms compared to the reference standard of Ciprofloxacin and Amphotericin...
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Posted by admin on Jan 31, 2021 in |
Development and validation of a simple UV spectroscopic method for the determination of Duloxetine by the derivative spectro-photometric method is the objective of the present work. The analysis was carried out by using Shimadzu UV-1700, and Shimadzu UV-1800 UV spectrophotometer, and Acetonitrile and water (8:2) was used as solvent or mobile phase. Detection or qualitative analysis was carried out at a wavelength of 240 nm. ICH guidelines were followed to verify the method validation process by using various parameters like linearity, accuracy, precision (interday and intraday), ruggedness, robustness, the limit of detection (LOD), and limit of quantification (LOQ). According to Beer’s Lambert’s law, the derivative UV spectrophotometric method was found linear in the range of 10-50 μg/ml. The analytical method was rugged and robust with a % relative standard deviation of less than 1.8. The percentage recoveries were higher than 98.7% in all the validation parameters and conditions. Based upon the analytical experiment, the developed method was found accurate, precise and rapid, and suitable for the rapid determination of...
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Posted by admin on Jan 31, 2021 in |
Co-crystallization is one of the most reliable alternative approaches to increase the solubility of poorly water-soluble drugs without affecting their physicochemical properties. Pharmaceutical cocrystals are neutral organic compounds connected to the API preferentially by loosely formed bonds as dipole-dipole interaction. Our present study aims at improving the solubility of an effective oral hypoglycemic, Glimepiride, a sulphonylurea class of drug that often lacks water solubility. In the process of enhancing its solubility, four different co-formers were used as Anthranilic acid, Succinic acid, Salicylic acid, Benzoic acid, and Gallic acid in different stoichiometric ratios like 1:2 and 1:3. The technique opted for the making is slow evaporation and prepare the nanoparticle by using Chitosan and gelatin polymeric matrix with aldehydic oxidized Xanthan gum as crosslinking agent. Initial confirmation was made through melting point determination. Later structure elucidation of co-crystals was carried out by several analytical methods, such as FTIR, X-ray Diffraction. In FTIR spectra, a sharp decrease in the intensity of N-H peak of salicylic acid and succinic acid was observed...
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Posted by admin on Jan 31, 2021 in |
Medicinal plants are often used by humankind since their evolution. They are known to have prominent therapeutic potential. The present work is conducted on Glycosmis pentaphylla (Rutaceae) an ethnobotanically important medicinal plant rich in secondary metabolites. The plant possesses metabolites like alkaloids, flavonoids, terpenoids, etc., which can be used against various illnesses. The plant is traditionally used by the folklore people in many parts of the world. The aim of the study is to investigate the bioactive compounds present and to evaluate the significance of the therapeutic and pharmacological uses of the phytoconstituents. All the standard phytochemical and spectroscopic procedures were followed for the detection and estimation of phytoconstituents. The leaves, stem, and root of Glycosmis pentaphylla were collected and was extracted using various solvents based on their polarity. Preliminary phytochemical analysis reveals the occurrence of several secondary metabolites. The quantitative estimation exhibited the presence of secondary metabolites abundantly in leaves on comparison with stem and root. Further, an HPTLC analysis to determine the number of phytoconstituents was also...
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Posted by admin on Jan 31, 2021 in |
A series of metal complexes, namely, Ni (II), Zn (II), Cu (II) and Pt (II), have been synthesized with newly prepared biologically active ligands. These ligands and their functional groups were carefully designed and selected to display DNA cleavage effect. Schiff base compoundof 2 – methyl – 3 – aminobenzopyrimidine – 4 – one (L1) was prepared by condensation of an equimolar mixture of 2-methyl-3-aminobenzopyrimidine-4-one (0.01 mole) and 2-hydroxybenzaldehyde (0.01 mole) in ethanol (25 ml). Schiff base (L2) compound was prepared by condensation of a mixture of Schiff base (1) (0.02 mole) and o-phenylenediamine (0.01 mole) in ethanol (25 ml) and a catalytic amount of KOH. The mixture was refluxed for 36 hrs. Ligand L2 (1 mmole) was dissolved in hot methanol. The metal chloride (1 mmole) was then added and the mixture was stirred under reflux for 24 h. The structures of ligands and their complexes have been confirmed by spectroscopic data i.e. IR, 1HNMR, MS, electronic and elemental (CHN) analysis. All Compounds were screened for DNA cleavage...
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