Posted by admin on Jan 31, 2021 in |
Two species of freshwater fish, Rasbora daniconius and Puntius ticto, were procured from a local freshwater body. They were subjected to chronic exposure by a daily sub-lethal dose of PCB 126, by use of a continuous flow-through method, for a period of 30 days. Estimation of bioaccumulation of PCB 126 in the intestine tissues of the fish was carried out at intervals of every 5 days using suitable clean-up methods. The extent of bioaccumulation of PCB 126 per gram wet weight of tissue in the eluate obtained after the cleanup process was assessed with the help of GC-MS. Both species of the test fish showed bioaccumulation in varying concentrations in the intestine tissue. A comparison of the PCB 126 values at the end of the experimental period of 30 days shows that there is an appreciably greater extent of bioaccumulation of PCB 126 in intestine tissue of the freshwater fish, Rasbora daniconius as compared to that of Puntius...
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Posted by admin on Jan 31, 2021 in |
The antimicrobial activity of Urtica dioica L. and Urtica urens L. has been reported; however, there are no reports regarding Urtica mexicana Liebm., which is widely distributed in central Mexico. U. urens and U. mexicana were collected in Amecameca and U. dioica was collected in Toluca Valley, both located in the state of Mexico. Crushed leaves of the plants were soaked consecutively in hexane, dichloromethane, methanol and water. The minimum inhibitory concentration (MIC) was determined with 0.039 mg/mL to 5 mg/mL solutions of the extracts, which were added to cultures of Salmonella typhimurium, Shigella flexneri, Salmonella typhi, Escherichia coli, Proteus mirabilis, Bacillus subtilis, and Staphylococcus aureus (5 × 106 CFU/mL) with Resazurin sodium salt and Mueller‒Hinton agar. The lowest MIC (0.039 mg/mL) was observed for the hexane, dichloro-methane, and the methanol extracts of U. urens against Bacillus subtilis and the hexane and dichloromethane extracts of U. urens against S. aureus (0.156 mg/mL). U. mexicana showed a greater spectrum of activity at higher concentrations; U. dioica showed exhibited the least...
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Posted by admin on Jan 31, 2021 in |
N’,N”’-((1E,1’E)-(propane-1,3-diylbis (sulfanediyl)) bis (1-(4-bromophenyl) ethan-2- yl-1-ylidene)) bis (2-hydroxybenzohydrazide) (BAPSSHZ) is found to be a selective and sensitive analytical reagent for the determination of trace levels of nickel (II) in environmental samples like medicinal plant materials and water samples (potable and polluted) by spectro-photometry. BAPSSHZ reacts with Ni (II) at pH 5.0 in aqueous DMF to form a yellow-colored (Ni-BAPSSHZ) complex that shows maximum absorbance (λmax) at 343 nm. Immediate color reaction and the absorbance remain constant for about 48 h. The molar absorptivity coefficient and Sandell’s sensitivity are found to be 7.21×104 L.mol-1 cm-1 and 0.064 µg cm-2 of Ni(II), respectively. The system obeys Beer`s law in the range of 2.37-23.71 µg mL-1 of Ni (II). The linear regression coefficient is 0.9991, and the standard deviation is 0.01086. A large number of cations, anions, and complexing agents do not interfere in this method. The developed method is applied for the determination of Ni(II) in medicinal plant materials and water samples (potable and...
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Posted by admin on Jan 31, 2021 in |
The present study aimed at analyzing the effects of PUFA in D-galactose treated Wistar albino male rats by determining leukocyte telomere length using real-time quantitative PCR, Il-6, and high sensitive C-RP levels estimation using ELISA. D-galactose is administered for 42 days orally (100 mg/kg), and on 43rd-day, blood is collected from tail vein and plasma is separated and subjected for IL-6 and high sensitive C-RP estimation using ELISA and determination of length of leukocyte telomere by real time qPCR. PUFA had no significant influence on length of leukocyte telomere and it decreased significantly IL-6 and high sensitive C-RP levels, indicating possible anti-aging effects by decreasing inflammation associated with aging...
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Posted by admin on Dec 31, 2020 in |
Introduction: Vernonia arborea, belonging to the Asteraceae family, found in the Western Ghats of India and Sri Lanka, was traditionally used in wound healing. It is proven that some of the plants of this genus have anti-diabetic properties. e.g., V. amygdalina. So this study was done to screen and to evaluate the anti-diabetic potency of the leaf extract of Vernonia arborea in diabetic Zebrafish models. Materials and Methods: Extraction was done by cold percolation method. The toxicity of the extract was evaluated, and the concentrations of 25 mg/l and 50 mg/l of tank water were taken for treatment as higher concentrations were found to be toxic. The Zefrafish were divided into the respective control and treatment groups. The different groups were induced with diabetes by high-glucose feed for 30 days. The standard group was treated with metformin and the test groups with the chosen concentrations of the extract for 7 days. The blood glucose level was checked on alternate days for 7 days. On the 7th day, the liver...
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