Posted by admin on Jan 31, 2021 in |
Many infectious diseases have been treated with natural medicine. Now, fungi metabolites continue to play a major role as a therapeutic medicine in many countries. The present study reveals the potentiality of filtrate of Aspergillus terreus and Cladosporium cladosporioides as antidermatophitic agents. Molecular identification of isolates fungi using DNA sequencing of EF4 and ITS4primerswere performed. The fungal filtrate of A. terreus and C. cladosporioides were prepared in Potato dextrose broth and Sabouraud dextrose broth media. Antidermatophitic activity of it was evaluated against Trichophyton mentagrophytes, Trichophyton verrucosum, Microsporum gallinae, Microsporum gypseum, Microsporum canis, Microsporum floccosum, Candida albicans, and Candida tropicalis using dry weight method. The results show that E. floccosum was the most sensitive for Sabouraud dextrose broth filtrate of A. terreus while M. gallinae was the most sensitive for Potato dextrose broth of A. terreus. The potato dextrose broth filtrate of A. terreus was further subjected to the determination of the Minimal inhibitory concentration using different concentrations. The minimal inhibitory concentration values of different extracts were found to be...
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Posted by admin on Jan 31, 2021 in |
Gastrointestinal tract disorders occur due to an imbalance of gastric acid secretion and defensive factors. Histamine receptor blockers and proton pump inhibitors are the most prominent therapies in the treatment of GI tract disorders. Inhibition of the K+/H+ ATPase pump blocks the secretion of gastric acid (HCl) from the parietal cells. The adverse effects of currently available drugs have obligated the screening of herbs as potent alternate medicines. In addition, plants contain different phytoconstituents, which are responsible for increasing defensive mechanisms against GI tract ulcers. Herein, we screen for the inhibition of K+/H+ ATPase pump using the methanolic extract of Clerodendrum inerme leaves. The extract exhibited 62.9% of inhibition (250 μgm) in a dose-dependent manner with an IC50 of 20.560 μgm/ml. Omeprazole (0.2mg/ml) was used as a positive control for the study. GC-MS analysis confirmed the presence of Phytol, a diterpenoid, as a major constituent in the methanolic extract. Hence, terpenoids could be attributed to the hypoacidic activity by inhibiting K+/H+ ATPase enzyme....
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Posted by admin on Jan 31, 2021 in |
Moringa oleifera is widely used in Asia and Africa as an anti-inflammatory, anti-diabetic, and cold agent. However, there are no Moringa medicines on the Russian pharmaceutical market; therefore, it is of interest for research as a promising source of raw materials and introduction to the State Pharmacopoeia of Russian Federation. This study was conducted to compare the characteristics of the authenticity of leaves from different regions. Moringa whole leaf from India and powder from Namibia were studied using macroscopic, microscopic, and qualitative chemical analysis. The results showed that there are both similarities and differences of characters depending on the area of collection of raw materials. A quantitative assessment of diagnostic signs was carried out; for the first time, trichomes and druses, as well as stomata. Qualitative phytochemical analysis revealed the presence of tannins, flavonoids, and saponins in both samples. It was established that the studied samples corresponded to pharmacopoeial requirements for authenticity indicators. A relatively larger number of calcium oxalate druses was found in the Indian raw materials. The...
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Posted by admin on Jan 31, 2021 in |
Daclatasvir is an antiviral drug used in combination with other drugs includes sofosbuvir, ribavirin, and interferon, depending on the virus type to treat cirrhosis caused by hepatitis C (HCV). Several methods have been found for quantification, but those are not cost-effective, and they are time-consuming. The present study developed a simple, precise, accurate and cost-effective UPLC method to determine daclatasvir quantity in tablet dosage forms. A simple and selective UPLC method is described for the determination of Daclatasvir Chromatographic separation was achieved on a Acquity BEH C18 (50 × 3.0mm. 1.7 µm) using a mobile phase consisting 0.1% of Orthophosphoric acid: Acetonitrile in a ratio of 60:40 v/v with detection of 248 nm. Linearity was observed in the range 50-150 µg/ml for Daclatasvir (r2 = 1.000). The amount of drugs estimated by the proposed method was in good agreement with the label claim. The proposed method was validated as per ICH guidelines and applied for the determination of the cited drug in the dosage...
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Posted by admin on Jan 31, 2021 in |
A greenhouse and field experiment was conducted to investigate the effects of Azotobacter (Azotobacter chroococcum), Phosphate solubilizing bacteria (Pseudomonas fluorescens), and Arbuscular mycorrhiza-like fungi (Sebacinca vermifera) on seed germination, physiology, and essential oil production of dill (Anethum graveolens). The experimentation was comprised of a randomized complete block design with six replicates of each treatment. Evaluation of germination was done using parameters; germination percentage, mean germination time, germination index, T50 of germination, seedling vigour, and vigour index. Physiological responses include the parameters for total length, total fresh weight, and total dry weight of the plant. For physiological evaluation, harvesting of plants was done a total of four times at an interval of 30 days i.e. on 30, 60, 90, 120 days after transplantation. Essential oil analysis was done by hydrodistillation followed by Gas chromatography-Mass spectrometry. All the data collected were statistically analyzed using the GraphPad Prism v6 software package. Results showed that all treatments negatively affected the emergence behavior of seeds, whereas physiology of plants and essential oil production by...
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