Posted by admin on Jan 31, 2021 in |
Gastrointestinal tract disorders occur due to an imbalance of gastric acid secretion and defensive factors. Histamine receptor blockers and proton pump inhibitors are the most prominent therapies in the treatment of GI tract disorders. Inhibition of the K+/H+ ATPase pump blocks the secretion of gastric acid (HCl) from the parietal cells. The adverse effects of currently available drugs have obligated the screening of herbs as potent alternate medicines. In addition, plants contain different phytoconstituents, which are responsible for increasing defensive mechanisms against GI tract ulcers. Herein, we screen for the inhibition of K+/H+ ATPase pump using the methanolic extract of Clerodendrum inerme leaves. The extract exhibited 62.9% of inhibition (250 μgm) in a dose-dependent manner with an IC50 of 20.560 μgm/ml. Omeprazole (0.2mg/ml) was used as a positive control for the study. GC-MS analysis confirmed the presence of Phytol, a diterpenoid, as a major constituent in the methanolic extract. Hence, terpenoids could be attributed to the hypoacidic activity by inhibiting K+/H+ ATPase enzyme....
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Posted by admin on Jan 31, 2021 in |
Moringa oleifera is widely used in Asia and Africa as an anti-inflammatory, anti-diabetic, and cold agent. However, there are no Moringa medicines on the Russian pharmaceutical market; therefore, it is of interest for research as a promising source of raw materials and introduction to the State Pharmacopoeia of Russian Federation. This study was conducted to compare the characteristics of the authenticity of leaves from different regions. Moringa whole leaf from India and powder from Namibia were studied using macroscopic, microscopic, and qualitative chemical analysis. The results showed that there are both similarities and differences of characters depending on the area of collection of raw materials. A quantitative assessment of diagnostic signs was carried out; for the first time, trichomes and druses, as well as stomata. Qualitative phytochemical analysis revealed the presence of tannins, flavonoids, and saponins in both samples. It was established that the studied samples corresponded to pharmacopoeial requirements for authenticity indicators. A relatively larger number of calcium oxalate druses was found in the Indian raw materials. The...
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Posted by admin on Jan 31, 2021 in |
Daclatasvir is an antiviral drug used in combination with other drugs includes sofosbuvir, ribavirin, and interferon, depending on the virus type to treat cirrhosis caused by hepatitis C (HCV). Several methods have been found for quantification, but those are not cost-effective, and they are time-consuming. The present study developed a simple, precise, accurate and cost-effective UPLC method to determine daclatasvir quantity in tablet dosage forms. A simple and selective UPLC method is described for the determination of Daclatasvir Chromatographic separation was achieved on a Acquity BEH C18 (50 × 3.0mm. 1.7 µm) using a mobile phase consisting 0.1% of Orthophosphoric acid: Acetonitrile in a ratio of 60:40 v/v with detection of 248 nm. Linearity was observed in the range 50-150 µg/ml for Daclatasvir (r2 = 1.000). The amount of drugs estimated by the proposed method was in good agreement with the label claim. The proposed method was validated as per ICH guidelines and applied for the determination of the cited drug in the dosage...
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Posted by admin on Jan 31, 2021 in |
A greenhouse and field experiment was conducted to investigate the effects of Azotobacter (Azotobacter chroococcum), Phosphate solubilizing bacteria (Pseudomonas fluorescens), and Arbuscular mycorrhiza-like fungi (Sebacinca vermifera) on seed germination, physiology, and essential oil production of dill (Anethum graveolens). The experimentation was comprised of a randomized complete block design with six replicates of each treatment. Evaluation of germination was done using parameters; germination percentage, mean germination time, germination index, T50 of germination, seedling vigour, and vigour index. Physiological responses include the parameters for total length, total fresh weight, and total dry weight of the plant. For physiological evaluation, harvesting of plants was done a total of four times at an interval of 30 days i.e. on 30, 60, 90, 120 days after transplantation. Essential oil analysis was done by hydrodistillation followed by Gas chromatography-Mass spectrometry. All the data collected were statistically analyzed using the GraphPad Prism v6 software package. Results showed that all treatments negatively affected the emergence behavior of seeds, whereas physiology of plants and essential oil production by...
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Posted by admin on Jan 31, 2021 in |
Inflammation, activated by oxidative stress, is the leading cause of all chronic diseases. Our culinary practices should include antioxidant and anti-inflammatory foods to combat these problems. Spices in India have been used traditionally not only as flavour enhancers in food but also as natural therapeutic agents in the prevention and treatment of a wide variety of ailments owing to the presence of an array of bioactive principles. The aim of the present study was to compare the in-vitro antioxidant, anti-inflammatory, and GC-MS profiles of Nigella sativa and Trigonella foenum-graecum seeds. In-vitro antioxidant and anti-inflammatory assays like DPPH (2, 2 – diphenyl – 1- picrylhydrazyl), Phosphomolybdenum, Ferric reducing antioxidant power, Superoxide radical scavenging assay, and Human Red Blood Cell stabilization assay was conducted to analyze the antioxidant and anti-inflammatory activities. Nigella sativa seeds were observed to have more antioxidant properties than Trigonella foenum-graecum seeds in all the assays. Maximum antioxidant potential was observed for Nigella sativa seeds in superoxide radical scavenging assay with lowest IC50 value of 17.28 µg/mL. The...
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