Posted by admin on Oct 31, 2020 in |
Rutin trihydrate a herbal drug having abundant medicinal properties, mainly used for topical treatment of inflammation, presents a hindrance and reduced anti-inflammatory activity. It is a BCS class II drug having low oral bioavailability, poor water solubility (0.125 g/L), and low stability of rutin in aqueous alkaline medium, restricting its application in use. So, it is successfully formulated as an emulgel to improve its anti-inflammatory properties. The current study aims to design, formulate, and to optimize Rutin trihydrate emulgel to enhance its efficacy and stability. Emulgel was designed, optimized by using central composite design taking three independent variables and one dependent variable. Five responses were generated, and the optimized formulation was prepared using carbopol 934(0.8%), clove oil (12.5 ml), and span (2.32%). The emulsifying agent being Tween 20, Span 20, and preservatives used are methyl and propyl parabens. The formulation (F2) with this combination gave emulgel of yellow color, homogeneous with good consistency, possessed pH of 6.51 ± 0.04, drug content of 98.7%, a viscosity of 14, 980 cps,...
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Posted by admin on Oct 31, 2020 in |
Miconazole nitrate is a broad-spectrum antifungal agent, and it is used for the treatment of superficial fungal infections. It has low skin permeability. Therefore, the aim of this study was to prepare proniosomal 2% miconazole nitrate sustained release to a treat deep-seated fungal infections. The different batches of proniosomal 2% miconazole nitrate gel were prepared by the conservation phase separation method using different non-ionic surfactants and Cholesterol. Preliminary trial batches were formulated and evaluated for different evaluation parameters like pH, viscosity, % entrapment efficiency, % drug content, and in-vitro drug release study. A 32 full factorial design was used to check the effect of Span 60 (X1) and Cholesterol (X1) on % entrapment efficiency (EE) and % drug release at 20 h (Q20). Multiple linear regression analysis, ANOVA, and graphical representation of the influence factor by 3D response surface plots were performed using Design Expert 9. Checkpoint batch was prepared to validate the evolved model. Optimized batch was found to be stable, and it showed release 94.76% in 24...
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Posted by admin on Oct 31, 2020 in |
Introduction: There are so many promising plant-based chemical constituents are present which act as an alternative therapy for the control of diabetes. But due to the lack of its proper quality control parameters, they are not widely used. The USFDA patent is approved for a fixed dosage combination of Mangiferin (MF) and Berberin HCl (BER) as antidiabetic herbal formulation. Objective: The literature review suggested that no UV spectroscopic method been reported in the literature review for the combination formulation of MF and BER. Methodology: The method is based on a simultaneous method using two wavelengths, 257 nm (λmax of MF) and 265 nm (λmax of BER). Methanol was used as a solvent. Results: The drug response with respect to absorbance was linear over the concentration range 5-30 μg/ml for MF and 10-60 μg/ml for BER. The percentage recovery of MF and BER as found to be 100.00% and 99.48%, respectively. The % R. S. D. values for intra-day, and the inter-day precision study was less than 2.0%, confirming that...
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Posted by admin on Oct 31, 2020 in |
The endophytic fungi isolated from the medicinal plant are an essential part of the current drug discovery from natural products. The endophytic fungus isolated from the healthy leaves of Psidium guajava was identified based on microscopic and cultural characteristics. The phylogenetic analysis inferred based on the internal transcribed spacer rRNA region sequence showed close similarity to Fusarium oxysporum. The secondary metabolite produced in solid-state, and submerged fermentation was extracted using ethyl acetate and acetone. The antimicrobial activity of the crude extract was screened by the agar well diffusion method. The inhibitory effect of the extract against bacterial pathogens was evaluated by minimum bactericidal concentration MBC and microdilution MIC methods. The antibacterial activity of the crude extract is significant at p<0.05 level. The extract presented a broad spectrum of inhibition against all the tested bacteria with inhibition zone diameter from 6.67 ± 0.58 mm to 22.00 ± 1.00 mm. The extract showed strong antimicrobial activity with MIC value from 0.156 mg/ml to 5.0 mg/ml and the MBC from 0.625 mg/ml...
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Posted by admin on Oct 31, 2020 in |
Pseudomonas aeruginosa is an opportunistic pathogen which acquires resistance to antibiotics such as carbapenems and cefepime. Information regarding (NDM-1) gene producing Pseudomonas aeruginosa is available, but data regarding their degree of infection and percentage in hospital settings are scarce. Hence, this study was carried out to determine the occurrence of blaNDM-1 gene among clinical isolates of multidrug-resistant Pseudomonas aeruginosa in a tertiary care hospital in Chennai, Tamil Nadu, India. A total of 126 isolates of P. aeruginosa isolated from various clinical samples were evaluated for carbapenem resistance and MBL production. In the present study it was found that majority of isolates (39.7%) were resistant to imipenem followed by ertapenem (35.9%) and doripenem (24.3%). A total of 31/78 (39.7%) isolates of P. aeruginosa were resistant to one or both carbapenems (imipenem and ertapenem) used in the screening test. Out of 78 isolates, 27 (34.6%) and 25 (32.1%) isolates were MHT positive and MBL producers by Modified Hodge Test (MHT) and MBL E-Strip test respectively and Out of 25 MBL positive...
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