Posted by admin on Jan 31, 2021 in |
The present study aimed at analyzing the effects of PUFA in D-galactose treated Wistar albino male rats by determining leukocyte telomere length using real-time quantitative PCR, Il-6, and high sensitive C-RP levels estimation using ELISA. D-galactose is administered for 42 days orally (100 mg/kg), and on 43rd-day, blood is collected from tail vein and plasma is separated and subjected for IL-6 and high sensitive C-RP estimation using ELISA and determination of length of leukocyte telomere by real time qPCR. PUFA had no significant influence on length of leukocyte telomere and it decreased significantly IL-6 and high sensitive C-RP levels, indicating possible anti-aging effects by decreasing inflammation associated with aging...
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Posted by admin on Dec 31, 2020 in |
Introduction: Vernonia arborea, belonging to the Asteraceae family, found in the Western Ghats of India and Sri Lanka, was traditionally used in wound healing. It is proven that some of the plants of this genus have anti-diabetic properties. e.g., V. amygdalina. So this study was done to screen and to evaluate the anti-diabetic potency of the leaf extract of Vernonia arborea in diabetic Zebrafish models. Materials and Methods: Extraction was done by cold percolation method. The toxicity of the extract was evaluated, and the concentrations of 25 mg/l and 50 mg/l of tank water were taken for treatment as higher concentrations were found to be toxic. The Zefrafish were divided into the respective control and treatment groups. The different groups were induced with diabetes by high-glucose feed for 30 days. The standard group was treated with metformin and the test groups with the chosen concentrations of the extract for 7 days. The blood glucose level was checked on alternate days for 7 days. On the 7th day, the liver...
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Posted by admin on Dec 31, 2020 in |
Suppression of wound repair is a challenge for patients and healthcare workers globally. Excision and incision wounds were created on Wistar albino rats on the back of the experimental animals. The rats were categorized into seven groups (n = 6). The study aimed to determine the effect of topical administration of successive extracts of Ventilago calyculata (stem bark) against dexamethasone-suppressed wound healing. The rats of Group I (normal – given saline and wounds covered with hydrogel base); Group II (positive control was given dexamethasone (1 mg/kg/body weight) intraperitoneally (10 days) and wounds covered with SSDee Ultra cream); Group III (untreated was given dexamethasone (1 mg/kg/body weight) intraperitoneally (10 days) and wounds covered with hydrogel base; Group IV-VII (treatment groups were given dexamethasone intraperitoneally (10 days) (1mg/kg/body weight) and wounds covered with F1, F2, F3, and F4 respectively. Excision and incision wounds were inflicted on the next day of dexamethasone dosing. At the end of the 30-day treatment period, wound contraction, epithelisation period, hydroxyproline content, tensile strength, and histological changes...
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Posted by admin on Dec 31, 2020 in |
Sphagneticola trilobata L. has been used to treat various diseases in worldwide. This study investigated the antioxidant activity and phytochemical constituents in chloroform and ethanol extract of Sphagneticola trilobata L. The extract of Sphagneticola trilobata L. was screened for antioxidant potential through DPPH assay, FRAP radical scavenging assay and phytochemical analysis was carried out for the detection of active secondary metabolite or different constituents such as alkaloids, flavonoids, proteins, carbohydrates and phenolic compound. Different concentrations of extract were analyzed and ascorbic acid was used as a standard antioxidant. IC50 and % inhibition were evaluated. S. trilobata L. leaves extracts of methanol and chloroform was found to be highly significant with the least IC50 value ranging from 68.0 to 75.0 µg/ml in DPPH. Chloroform and methanol extracts of S. trilobata L. leaves showed excellent FRAP free radical scavenging activity with IC50 value ranging from 0.55 ± 0.042 and 0.55 ± 0.03 respectively and ascorbic acid 0.63 ± 0.01 and the extract was found to contain large amounts of secondary metabolites...
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Posted by admin on Dec 31, 2020 in |
The stem bark of Khaya senegalensis, known as mahogany is commonly sold on the Ghanaian market for the treatment of numerous ailments. To ensure its safe and efficacious use, there is a need to ensure that this plant material is of the best quality. Quality will involve ensuring its identity, purity and content are up to recommended standards by applying available and relevant methods. As a means to improve the safe use of the material, mutagenic studies were also conducted. This study sought to evaluate parameters that can be easily applied to evaluate the quality of the stem bark of K. senegalensis. Macroscopy, microscopy, physicochemical, and phytochemical investigations using fluore-scence and UV analysis were employed. Microscopic analysis of K. senegalensis leaf revealed the presence of calcium oxalate crystals, trichomes, stomata cells, and vein islets. Phytochemical screening of the stem bark showed the presence of alkaloids, saponins, tannins, flavonoids, glycosides, and terpenes. UV analysis displayed spectra that shift and change in absorbance on acidification and basification of the aqueous and...
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