Posted by admin on Sep 30, 2020 in |
A simple, sensitive, and rapid reverse phase High-Pressure Liquid Chromatography (HPLC) method was developed and validated for the quantification of residual Dicyclohexylurea in Fosaprepitant dimeglumine drug substance at trace level. The method was optimized on Zorbax Eclipse XDB-C8, 250 mm × 4.6 mm, 5µm with column oven temperature maintaining at 35 °C with flow rate at 0.7 ml/min. Mobile Phase was prepared by a mixture of phosphate buffer pH 7.0 (0.68 g of Potassium dihydrogen orthophosphate in 1000 ml of water) and Acetonitrile in the ratio of 53:47 v/v), the injection volume is 20 µl, the detector wavelength is 205 nm. The developed, optimized method was further validated in accordance with ICH, and the method is found to be specific, sensitive, accurate, and precise. The Limit of detection (LOD) and Limit of quantification (LOQ) for dicyclohexylurea are 20 µg/g and 60 µg/g, respectively. The method validation experimental results are discussed in detail in this research...
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Posted by admin on Sep 30, 2020 in |
At present more than 200 oral continued/controlled/ expanded/altered discharge details are accessible in the worldwide market. In India, notwithstanding, just constrained items are financially affordable. Making a stride ahead from assembling traditional dose structures, the advancement of controlled drug delivery systems. Controlled drug delivery systems have been created to improve the following arranging of the medication in the body. They can assume a noteworthy job in a focused medication conveyance framework in organ or tissue. In the Controlled medication conveyance framework, more than one instrument might be included at various phases of medication pharmacokinetics and pharmacodynamics profiling. Some medication conveyance frameworks have been planned and are being researched. These sorts of the context had some bit of leeway over common medication conveyance framework, including a brief time of medication discharge insurance of flimsy medications and expanded patient solace and consistency. The audit underlines the technique of controlled medication conveyance framework planning, their criticalness, burdens, point by point order, and the pertinent model any place required are illustrated. This audit...
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Posted by admin on Sep 30, 2020 in |
Merremia emarginata is a creeping herb, rooting at nodes, and commonly used in folk medicine. The current study was undertaken with the aim of preliminary phytochemical analyses, evaluation of antimicrobial activity, and antioxidant activity by methanol and aqueous extracts of leaves of Merremia emarginata. The extracts were subjected to preliminary phytochemical analyses; Antibacterial activities against nine bacterial strains were done by using agar – well diffusion method and free radical DPPH antioxidant activity. Preliminary phytochemical analyses were done by using standard phytochemical methods that showed the presence of Carbohydrates, reducing sugars, phytosterols, fixed oils, saponins, phenolic compounds and flavonoids. Based on the results of phytochemical analyses, methanol and aqueous extracts of the plant were chosen for antibacterial studies. Methanolic extract of leaves showed the highest antibacterial activity followed by the aqueous extract. The present study indicates that the leaves of Merremia emarginata possess antibacterial activity and found effective against eight bacterial cultures. Antioxidant activity of the DPPH assay showed that extracts exhibit the scavenging effect of various...
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Posted by admin on Sep 30, 2020 in |
HPTLC is simple, reliable, precise and accurate for separation and identification of drug in a combined dosage form which gives satisfactory accuracy in results when applied on amlodipine besylate (Amlo) and celecoxib (Celo). Toluene, ethyl acetate, methanol and conc. ammonia (6:5:1.5:0.3 v/v/v/v) was required as a mobile phase for separation and identification of drugs. These drugs were scanned by densitometry at 310 nm. The developed method was validated for linearity, precision, accuracy, LOD, and LOQ. The linearity range and correlation coefficient for Amlo and Celo were obtained to be 100 to 600 ng/ml and 2 to 12 μg/ml, 0.9971, and 0.9989, respectively. The results of the recovery study for Amlo and Celo have obtained the range of 99.52% to 99.83% and 99.83% to 99.98%, respectively. The method precision was established by repeatability study. LOD and LOQ are 17.88µg/ml and 54.20 µg/ml for Amlo and 0.39µg/ml and 1.20µg/ml for Celo, respectively. The statistical parameters and recovery data indicated that the method may be engaged for efficient, rapid drug analysis from...
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Posted by admin on Sep 30, 2020 in |
In the present study, eleven new chalcones bearing a methanesulfonyl group at para position have been synthesized by the condensation of 4-methylsulphonylacetophenone with different para-substituted aromatic aldehydes. The structures of the newly synthesized molecules were elucidated by spectroscopic methods such as as1H NMR, 13C NMR, and mass. These compounds were screened for their anti-inflammatory activity by carrageenan-induced rat paw edema and leucocytes migration methods and analgesic activity by acetic acid-induced writhing test in albino Wistar rats. Among the synthesized compounds, MS7 to MS10 which contain electron-withdrawing groups were found to be most active with reference to anti-inflammatory activity at a dose level of 10 mg/kg and caused a significant reduction in the number of recruited leukocytes and inhibition of pleural exudates formation that was comparable to that of indomethacin at a dose of 10 mg/kg. The compounds which contain halogens and methoxy groups showed a significant analgesic activity at a dose of 10 mg/kg when compared to standard diclofenac sodium. Thus, it can be concluded that among the compounds...
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