Posted by admin on Nov 30, 2020 in |
A simple, accurate, and precise stability-indicating reverse phase high pressure liquid chromatographic method was developed and validated for the estimation of Perindopril arginine, Indapamide, and Amlodipine in the bulk and pharmaceutical dosage form. Chromatographic separation was carried out on ZORBAX RX C8 (250 mm × 4.6 mm, 5 µm) as a stationary phase with a mobile phase of gradient system of Phosphate Buffer with pH 2 containing Decane Sulphonate as ion-pairing agent (A) and Acetonitrile (B) at detection wavelength 215 nm with flow rate 1.0 mL/min at column temperature 40 ºC. The tR of Perindopril, Indapamide, and Amlodipine was 31.39 ± 0.21 min, 25.73 ± 0.41 min, and 36.95 ± 0.47 min, respectively. The method was linear over the concen-tration ranges 50-150 µg/ mL for Perindopril, 12.5-37.5 µg/ mL for Indapamide and 50-150 µg/ mL for Amlodipine. The LOD was 0.99 µg/ mL for Perindopril, 0.87 µg/ mL for Indapamide and 3.99 µg/ mL for Amlodipine. The LOQ was 3.02 µg/ mL for Perindopril, 2.6 µg/ mL for Indapamide...
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Posted by admin on Nov 30, 2020 in |
Permethrin, a member of one of the newest class of insecticides, the synthetic type-I pyrethroids, is very much in use nowadays. Due to its relatively low mammalian toxicity and high photostability, it has earned great popularity. Several reports suggest its endocrine disrupting activity, damaging effect on nervous system, its receptors, and other adverse effects. However, very limited information is available regarding its effect on reproductive organs. It is, therefore, imperative to bridge these lacunae. Hence, the present study was aimed to evaluate the effects of Permethrin on the reproductive tissues of male mice. Permethrin (130 mg/kg body weight) was administered orally for a period of 21 days. Biochemical parameters were assayed in testis, caput epididymis, cauda epididymis, seminal vesicle, and vas deferens of Swiss albino mice. The results showed that Permethrin administration lowered the gravimetric indices and decreased the levels of enzymes involved in the energy metabolism like ATPase, ALKPase, ACPase, and SDH, which may lead to oxidative stress and hamper the overall physiological...
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Posted by admin on Nov 30, 2020 in |
The objective of the present investigation was to formulate the thermosensitive ophthalmic in-situ gels of Dorzolamide HCl for the treatment of glaucoma. Dorzolamide HCl is presently available as conventional eye drops, which has demerits like pre-corneal elimination, lachrymal drainage and no sustained effect leading to poor bioavailability and hence there was a need to develop a better, stable and sustained drug release dosage form. Dorzolamide HCl loaded thermosensitive ophthalmic in-situ gels were prepared by cold method using poloxamers as thermo-sensitive polymers in different concentrations with HPMC K4M as viscosifier which was used in three different concentrations. A total of six formulations were made and evaluated for para-meters, namely appearance, clarity, pH, gelling capacity, gelation temperature, rheological study, drug content, and in-vitro drug release studies. All the evaluation parameters were within limits. Among all six formulations, one (DF-5) showed the highest drug release, and this formulation was evaluated for sterility, isotonicity, and in-vitro ocular irritation study, where all these parameters were found to be satisfactory. The results of the current...
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Posted by admin on Nov 30, 2020 in |
The medicinal plants, Adhatoda vasica, and Adhatoda beddomei are commonly used in the treatment of several illnesses and respiratory disorders viz., cold, severe cough, chronic bronchitis, asthma and tuberculosis. The present study aimed to identify the phytochemical constituents and quantification of bioactive contents of hexane extracts of these plants, followed by metabolic fingerprinting, which was carried out by GC-MS analysis. Assessment of phytochemical screening showed the presence of various phytochemicals like glycosides, phytosterols, flavonoids, terpenoids, and phenols in hexane extract of A. vasica and A. beddomei. A rapid method has been used for the comparative study of A. vasica and its related plant species A. beddomei using gas chromato-graphy-mass spectrometry (GC-MS). The phytocompounds comparison in two plants by GC-MS was similar in 12 compositions. Squalene, β-sitosterol, tetratetracontane, and lupeol were the major constituents identified in the hexane extract of A. vasica and A. beddomei. The extracts showed the presence of several phytoconstituents, including bioactive components, and provide reference data for future research of its active...
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Posted by admin on Nov 30, 2020 in |
The objective of the present study is to evaluate the phytochemical constituents, antioxidant and antibacterial activities of methanolic leaf extract of Clinopodium nepeta (L.) Kuntze. Total phenolic content of leaf extracts was assessed using Folin-Ciocalteu and AlCl3 reagent. Antioxidant assays were evaluated using DPPH, ABTS radical scavenging, and ferric, reducing antioxidant power (FRAP) methods. The leaf extract was screened against four pathogenic bacteria, two gram-positive (Escherichia coli, Bacillus cereus) and two-gram negative (Pseudomonas aeruginosa, Klebsiellea pneumonia) using disc diffusion method. The results showed the total phenolic content (378.52mg gallic acid equivalent (GAE)/g) and flavonoid content (189.4 mg quercetin equivalents (QE)/g) of methanolic leaf extracts. DPPH and ABTS radical scavenging activity with an IC50 value of 0.49mg/ml and 1.68mg/ml, respectively. It also exhibited good absorbance in the FRAP assay. The in-vitro antibacterial assay showed an excellent zone of inhibition against Pseudomonas aeruginosa (19.01±0.3mm), Bacillus cereus (24.66±0.3mm), Klebsiellea pneumoniae (16.74±0.3mm), and Escherichia coli (17.56±0.31). Clinopodium nepeta (L.) Kuntze showed maximum antioxidant property and maximum inhibition against the test organisms. The high...
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