Posted by admin on Sep 30, 2020 in |
Cytochrome c oxidase, a mitochondrial metalloenzyme acting as the terminal enzyme of the mitochondrial respiratory chain. It provides a critical function in cellular respiration in both eukaryotes and prokaryotes. The recent development in technology has enabled researchers to understand the genetic, molecular, structural, and functional properties of proteins to identify appropriate targets against diseases, and as a result, many anti-inflammatory and anti-proliferative drugs have been developed. In the absence of X-ray and NMR protein crystal structure, homology modeling provides a useful 3D model for a protein that is related to at least one known protein structure. In the present study, the 3D molecular structures of nine Cytochrome c oxidase subunit 1 protein from different species such as Myxine glutinosa, Struthio camelus, Sus scrofa, Homo sapiens, Xenopus laevis, Halichoerus grypus, Zygogeomys trichopus, Buteo buteo, and Plasmodium falciparum were predicted using homology modeling software MODDLLER. The modeled structures were docked using Autodock4.2 software with ten different natural compounds and three drugs as a control to study the molecular interactions of these...
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Posted by admin on Sep 30, 2020 in |
The aim of the present research work is to isolate and characterize microbial strain using partial sequencing of 16S rRNA gene region of Amycolatopsis strain K1. Five Amycolatopsis strains from soil samples were collected from the effluent sediments from Jajmau, Kanpur in U.P. India, and it was confirmed by polymerase chain reaction (PCR) that the isolates were different and not re-isolates of the same strain. Sequence analysis of the PCR-amplified 16S rRNA gene indicated that isolates showed maximum similarity to Saccharopolyspora endophytica strain YIM 61095. Amycolatopsis strain K1, which is a gram-positive bacterium, was cultured in a nutrient medium containing cholesterol at 37 °C for 24 h. A partial sequence of 16S rRNA gene was amplified by PCR and sequenced; the sequence data were submitted to NCBI. Amycolatopsis strain K1 partial sequence of 16S rRNA gene region resulted in 1366bp. The sequence data was submitted to NCBI, and the accession number obtained is SUB 6244430. The phylogenetic tree was constructed using the neighbor-joining through multiple sequence alignment program of Mega...
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Posted by admin on Sep 30, 2020 in |
Cyanobacteria are a well-established source of antibacterial compounds. However, the hot spring cyanobacterial strains are not properly explored for the purpose. The study investigates the antibacterial potential of the thermophilic oscillatoriales isolated from a hot spring of Garhwal Himalaya. Out of 48 endo-metabolite pools, extracted from 16 thermophilic cyanobacterial strains, tested for antibacterial activity against the bacterial pathogens, the DEEL (diethyl-ether extract of Leptolyngbya sp. HNBGU 002) was found to be active against Staphylococcus aureus ATCC 25923 with the minimum inhibitory concentration of 2.0 mg ml-1 and was able to disrupt the cell membrane function of bacteria as evidenced by an increase in membrane conductivity. In addition, DEEL was also found to be active against MRSA (methicillin-resistant Staphylococcus aureus) and VRE (vancomycin-resistant Enterococcus faecium) strains. Gas chromatography-mass spectrometry analysis revealed the hydrocarbons and phenolics as the major compounds present in the DEEL. Besides, a triazolo-pyrimidine (TP) derivative, 1; 7-methyl-6-nitro[1,2,4] triazolo[1,5-a] pyrimidin-5-ol, was also detected in the active fraction. The findings suggest that the antibacterial activity of DEEL may be...
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Posted by admin on Sep 30, 2020 in |
Murivenna is a taila (oil) which is widely used in South-Indian practices for external application in many of the localized conditions such as trauma, inflammation of joint, fracture, joint dislocation, wound, etc. Taila (Oil) is considered best for vatahara action. Vata is the main dosha involved in shopha (inflammation); this is a suitable taila (oil) that works in the treatment of shopha (inflammation). Materials and Methods: Murivenna was prepared as per the guidelines of Trivandrum Pharmacopeia and Ayurveda Formulary of India with genuine drugs. A later experimental study was conducted in acute and chronic inflammation using Carrageenan-induced paw edema and cotton pellet granuloma pouch method in acute and chronic inflammatory study, respectively. The data were analyzed statistically using a one-way analysis of variance (ANOVA) followed by multiple comparison Dunnett’s test as a post hoc test. Result: The results indicate the presence of significant anti-inflammatory activity in the experimental model representing acute inflammation and non-significant effect in chronic inflammation. But there is a decrease in the granuloma formation, which suggests...
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Posted by admin on Sep 30, 2020 in |
The incorporation of Ordinary Portland cement with additional cementing materials is one of the most efficient methods to improve its microscopic properties. The aim of this work is to investigate the effect of metakaolin on Portland cement performance by using theoretical methods. 14 Å tobermorite was used as an alternative familiar molecular structure in representing Ordinary Portland cement. A constituting theoretical model has been constructed to describe the structure of Portland cement mixed with different ratios of metakaolin. This model was subjected to molecular dynamics simulations, and force field methodologies were employed to investigate the mechanical properties behavior. The results revealed an effective influence of metakaolin on the mechanical stiffness properties of Portland cement. The values of mechanical stiffness parameters (Bulk, Shear, and Young’s Modulus) were increased by increasing the metakaolin...
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