Posted by admin on Jun 30, 2020 in |
Pseudomonas aeruginosa is one of the most emerging multidrug-resistant opportunistic pathogens, which poses a threat to immunocompromised patients. Treatment of P. aeruginosa in the affected patients has become difficult because of its ability to develop resistant strains against most of the conventional therapeutic antibiotics. Quorum sensing plays a crucial role in the virulence nature of P. aeruginosa as it helps the bacteria to produce virulence factors and biofilm formation. In the present study, we studied the quorum quenching potential of fungal secondary metabolites from the well-known fungus Phoma multirostrata PUTY3 strain isolated from Carica papaya. In this study, we have carried out both in-vitro and in-silico analyses of fungal secondary metabolites from P. multrirostrata against P. aeruginasa. The in-vitro analysis showed a marked decrease in the production of virulence factors such as pyocyanin, rhamnolipids, protease, elastase, exopolysaccharides, HCN gas production and biofilm formation. In addition to this, a significant inhibition in the motility factors of P. aeruginosa such as swimming, swarming, twitching was also observed. In-silico studies further validated...
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Posted by admin on Jun 30, 2020 in |
The objective of the present study is to evaluate phytochemical composition and HPTLC finger print profile of medicinally useful plant Martynia annua L. (Martyniaceae) root extracts. The CAMAG HPTLC system was used for the finger print profiling of root extract using the mobile phase chloroform: Methanol (9:1 v/v). The profile showed that the root extract of Martynia annua exhibited several peaks with different Rf values when visualized at 254 nm and 366 nm. At 254 nm a total 7 peaks were observed and at 366 nm 9 peaks were observed. The result of HPTLC analysis of Martynia annua root extracts shows that the maximum number of chemical constituents present in alcoholic root extract of Martynia annua in comparison to hydro-alcoholic and aqueous root extract of Martynia annua in given solvent system of chloroform and methanol. Further bioactivity guided fractionation and analysis of isolated chemical entity can reveal the active constituents in the different root extract of Martynia...
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Posted by admin on Jun 30, 2020 in |
The objective of the work was to formulate a self-nanoemulsifying drug delivery system (SNEDDS) of zaltoprofen and to study the impact and interaction of various formulation variables on the formulation by the design of the experiment. A 13-run 3-factors, 3-levels box behnken design (BBD) was used for optimization with 1 replicates at the center point. The concentration of phosal 53 MCT, tween 80, and PEG 400 as oil, surfactant, and co-surfactant, respectively, were used as independent variables. Self emulsification time, % transmittance, and relative turbidity were chosen as responses for the study. Results obtained show the impact of independent variables on the response under study. The optimal concentrations of variables were found to be 27.71% (w/w) oil, 46.1% (w/w) surfactant and 19.28% (w/w) co-surfactant. The in vitro dissolution study showed a marked increase in dissolution rate than zaltoprofen powder. The box Behnken design was successfully used in the optimization of zaltoprofen SNEDDS and to study the impact of components on the response under study in a simplified and time-effective...
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Posted by admin on Jun 30, 2020 in |
Parkinson’s infection is a neurodegenerative issue portrayed by the cardinal manifestations of solidness, resting tremor, gradualness (bradykinesia) and decrease of development (hypokinesia). It is a maturing populace and in spite of the fact that there have been a few significant leaps forward as far as the treatment of this crippling illness, for example, drugs levodopa, dopamine [DA] agonists, anticholinergic and medical procedure. Parkinson’s ailment is an interminable, dynamic, neurodegenerative confusion with an expected pervasiveness of 31 to 328 per100, 000 individuals around the world. It is evaluated that more than 1 percent of the populace over age 65 are burdened with Parkinson’s infection, occurrence and pervasiveness increment with age. So, the point of the investigation to assess the impact of daidzein on Parkinson sickness instigated by the reserpine model in rodents. Reserpine is the antihypertensive specialist, incites the consumption of focal catecholamines stores. Infusion of reserpine in rodents causes hypokinesia, unbending nature, tremors, and idleness. Cell reinforcements assume a fundamental job in the avoidance or treatment of Parkinson infection,...
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Posted by admin on Jun 30, 2020 in |
Phymatosorus scolopendria (Burm. F.) Pic. Serm. belongs to the family Polypodiaceae which is commonly known as musk-fern or wart-fern. Medicinally, the experimental plant possesses insect repellent, anti-inflammatory and bronchodilator activity and hence used ethnomedicinally by tribal people, but was not explored for its bioactive compounds. Hence, the objective of this study was to determine the bioactive compounds present in the methanolic extract of Phymatosorus scolopendria by Gas Chromatography-Mass Spectrum. The methanolic extract of the experimental plant revealed the presence of four phytoconstituents, namely dodecanoic acid, 1,2,3-propanetriyl ester, myristic acid vinyl ester, 4-nitrophenyl laurate and hexadecanoic acid, 4-nitrophenyl ester with peak values 8.98, 53.29, 26.91 & 10.82. The predicted biological activities for the present compounds identified when referred in the literature shows antioxidant, antibacterial, antiviral, candidicidal, hypocholestrolemic, antiarthritic, hepatoprotective, mosquito repellent, antimicrobial, anticancer and cosmetics. The compounds were identified by comparing their retention time and peak area with that of literature and interpretation of mass spectra. The research could be continued to find out the therapeutic importance of all the...
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