Posted by admin on Aug 31, 2020 in |
Simple, sensitive, and accurate spectrophotometry method one each for estimation of five drugs viz., chloroquine phosphate (CHP), granisetron hydrochloride (GRA), rizatriptan benzoate (RIZ), zoledronic acid (ZOL) and zolmitriptan (ZOT), have been developed. The method depends upon oxidation of each drug by excess N-Bromo succinimide (NS), and subsequent determination of UN reacted NBS by Rhodamine-B dye at λmax 557 nm. Beers law is obeyed between 2-14 µg mL-1 (for CHP); 52-14 µg mL-1 (for GRA); 1-7 µg mL-1 (for RIZ); 1-7 µg mL-1 (for ZOL) & 2-14 µg mL-1 (for ZOT). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. All the methods have been validated as per ICH...
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Posted by admin on Aug 31, 2020 in |
Alzheimer’s disease is the most common neurodegenerative disease, which initially causes oxidative stresses and leads to neuronal death. It characterized by notable memory loss, cognitive impairment, and personality disorders accompanied by diffuse structural abnormalities in the brain of the aged population. The present work focused on anticholinesterase inhibiting activity Smilax zeylanica and Smilax china against neurodegenerative disorder such as Alzheimer’s diseases on animal model respectively, as proof of preliminary anticholinesterase profile. In this paper, we studied various extracts prepared from roots of Smilax zeylanica and Smilax china for their AChE -inhibitory activity using animals. The ethanolic and various extracts from the plant roots of smilax zeylanica and smilax china were screened for their acetylcholinesterase inhibitory activity by using animals and Ellman’s method. These extracts of smilax china and smilax zeylanica exhibited inhibitory activities were observed (Cortex and Hippocampus) at a concentration of 150 mg/kg, it can be used for further study to treat the neurodegenerative disorders like Alzheimer’s disease...
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Posted by admin on Aug 31, 2020 in |
A simple, sensitive, and rapid Liquid Chromatography-Mass Spectroscopy method was developed and validated for the quantification of Isoproterenol in human plasma using Dobutamine as an internal standard. The method utilized simple liquid-liquid extraction using a mixture of diethyl ether and dichloromethane for the sample preparation involved prior to LCMS analysis. The analytes were chromatographed on Prontosil ODS C18 Column with isocratic elution using methanol, Acetonitrile and Tri ethyl-amine in the ratio of 60:25:15 (v/v) at pH 6.3 as the mobile phase at a flow rate of 0.9 mL/min and the UV detector response of the column eluents were recorded at a wavelength of 248 nm. Quantification was performed in multiple-reaction-monitoring mode with the ion transitions m/z 212.19 → 135.21 for Isoproterenol, m/z 302.19 → 107.05 for Dobutamine. Good linearity was obtained in the range of 0.50–300 ng/mL (r2 = 0.999). The method was fully validated with accuracy, precision, matrix effects, recovery and stability. The results of the stability study confirm that the method was found to be stable. The...
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Posted by admin on Aug 31, 2020 in |
In this planed research work, aryl piperazine derivatives will be synthesized because aryl piperazine currently the most important constructive block in drug discovery with positive pharmacological evaluation. A series N- (4- (benzo[d]thiazol-2-yl) phenyl)-2-[4-(aryl-substituted) piperazines-1-yl]acetamide, N-(4-(benzo[d]oxazol-2-yl)phenyl)-2-[4-(arylsubstituted)piperazines-1-yl]acetamide and Synthesis of N-(4-(benzo[d]imidazol-2-yl) phenyl)- 2- (4- (arylsubstituted)piperazin-1-yl) acetamide will be synthesized with their characterization such as melting point determination, Thin-layer chromatography (TLC) and spectral analysis. After that, pharmacological evaluation, such as antibacterial activity, will be performed for synthesized compounds. All the synthesized compounds were reported for determination of zone of inhibition (mm), minimum inhibitory concentrations (MIC) were also calculated for effective derivatives, with an objective to offer some potent antimicrobial agents to human beings. Antimicrobial activity is determined based on their in-vitro activity in pure cultures. In-vitro susceptibility testing is done by two methods i.e., Turbidimetric/photometric/tube dilution method and agar diffusion/cup-plate/cylinder plate...
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Posted by admin on Aug 31, 2020 in |
SARS-CoV-2 or COVID-19 is one of the deadly pandemics faced by the world population, which has infected 7 million and claimed the lives of 0.4 million people. In spite of a few drugs available to control the pandemics, a formal vaccine is the least that the world expects under the current scenario. However, the release of a vaccine is expected to come at the cost of its own time. SARS-CoV-2 replicates in the host cells with the aids of the molecular machinery of a complex formed by three non-structural proteins (NSPs) viz., nsp12, nsp8, and nsp7. Recent studies reveal that among the three NSPs, nsp12 is vital for viral replication and is the target for drugs. Several studies have linked the viral infection to a weaker immune system, which is quite likely to be targeted by the virus. In search of such a natural compound that might increase the immunity and block the viral replication within the host, we selected C-Phycocyanin of Spirulina plantesis to study its anti-viral property...
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