Posted by admin on Sep 30, 2020 in |
Gujarat has the second largest mangrove ecosystem in India, and mangrove ecosystems are known for their extreme environments and hence extremophiles that can produce novel enzymes can be found in these extreme environments. In this study, ninety-three bacterial isolates were obtained in pure cultures from soil samples collected from Kandla, Mundra and Tuna of the Kutch region of Gujarat in the winter season. These isolates were plate-screened for their ability to produce six extracellular enzyme activities using their respective methods in order to find new isolates for their possible applications. Six enzymes amylase, lipase, protease, cellulase, oxidase, and catalase were mostly present in these isolates. The isolates were also screened for their ability to produce the bioplastic Polyhydroxybutyrate (PHB). Further, these isolates were tested for their ability to survive at different salinity levels. Among the isolates, HK47, HM19, HTA1 and HT83 were the highest amylase, lipase, protease and cellulase producers respectively. Isolates HM27, HM82 and HTA2 had shown their polyenzyme activity. HT64 and HM88 were emerged as Halotolerant while...
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Posted by admin on Sep 30, 2020 in |
To examine the antioxidant property of ethanolic extract of roots of Smilax china (EESC) on Aluminium Chloride Induced oxidative stress in Wistar rats. The phytoconstituents are extracted from the roots of S. china using ethanol, by hot continuous percolation method. The rats were divided into 5 groups (6 animals/ group) and treated with EESC (150 & 300 mg/kg of body weight) and piracetam (0.5 mg/kg of body weight) for 14 days after inducing oxidative stress with AlCl3 (100 mg/ kg of body weight) for 60 days. The lipid peroxidation level (TBARS) and antioxidants activities like Superoxide dismutase (SOD), Catalase (CAT), and Glutathione (GSH) were estimated. When compared with the control group, AlCl3 induced rats showed increased TBARS and decreased antioxidant enzymes like Catalase (CAT), Superoxide dismutase (SOD) and reduced glutathione (GSH). The EESC at higher dose 300 mg/kg of body weight animals were significantly (P<0.001) reduced the TBARS and increased the antioxidant enzymes SOD, CAT, and increased GSH when compared with the control group. The present study results revealed...
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Posted by admin on Sep 30, 2020 in |
The main objective of this research work is to develop biorelevant dissolution method for the Memantine extended-release capsule by correlating pre-prandial in-vivo performance. The mean plasma concentration profile obtained after oral administration of extended-release capsules was deconvoluted using the Wagner-Nelson deconvolution technique for deriving the percentage fraction of drug absorbed to achieve the target dissolution profile. The dissolution results obtained by using the office of generic drugs recommended dissolution method was not comparable with the target dissolution profile and observed with an F2 value of 30. Biorelevant dissolution method was developed using USP Apparatus 3 (reciprocating cylinder), with dissolution media simulating gastrointestinal tract sink condition. A full factorial design of the experiment was carried out for dissolution method development and optimization. Media volume and dips per minute were considered as main factors in the design of the experimental study. The developed biorelevant dissolution method based on statistical interpretation of the main effect, interaction effect, and desirability factor is with media volume 150 ml and 15 dips per minute and...
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Posted by admin on Sep 30, 2020 in |
Mosquito-borne diseases are serious health problems in India, and mosquito vector control is a fundamental approach to control various diseases. Essential oils represent eco-friendly alternatives to costly synthetic pesticides for mosquito control. The essential oil of Mentha spicata in India was used for its activity against Ae. aegypti. The essential oil showed pupicidal activity against this mosquito. The results indicated that essential oil from M. spicata induced 29, 49.5, 60.8, 67.76, and 81.36% mortality at 100, 200, 300, 400 and 500 ppm essential oil concentration, respectively. The essential oil was rich for various phytochemicals, including estragole, thymol, (-) – 8 – p – Menthen – 2 – yl, acetate, trans, and 3, 5-Heptadienal, 2-ethylidene-6-methyl-. This essential oil may serve as a low-cost vector control agent for mosquito-borne...
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Posted by admin on Sep 30, 2020 in |
Forskolin is a heterocyclic labdane-type diterpenoid extracted from the roots of the plant of Coleus forskohlii (Lamiaceae) has been studied in depth for its broad range of pharmacological properties. Coleus forskohlii aids in the control of pestilence of Uzi fly, Exorista sorbillans, and Exorista bombycis one of the gravest ecumenical pests in sericulture industry. Forskolin affected moulting; development and metamorphosis of Uzi fly and induced various types of morphological abnormalities in Uzi fly. The treated resultants of fifth instar Uzi fly maggots, a few of them died during moulting, some of them developed into maggot-pupal and pupal-adult intermediates, abnormal pupae, abnormal adults and remaining treated fifth instar maggots developed into morphologically normal adults exhibiting reduced fecundity when compared to the controls. Coleus forskohlii root extract (CFRE) exhibited growth-regulating activity on the Uzi fly. They affected pupal- adult transformation and produced intermediates along with ovarian deformities deformed adults and also affected the inhibition of pupation and adult emergence. The plant extract repressed the growth and development suggesting its use for...
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