Posted by admin on Apr 30, 2020 in |
Aim and Objectives: The present study was an attempt to investigate the effect of Thetran vidhai kudineer (TVK) on streptozotocin (STZ) induced diabetes in male Wistar rats. Methods: TVK, is a Siddha polyherbal formulation containing four ingredients includes Strynchnos potatorum, Terminalia chebula, Cassia auriculata, and Limonia acidissima. 6-8 week old 30 male Wistar albino rats fed with normal diet ad libitum and free access to water containing 10% fructose (w/v) for 5 weeks. After five weeks of dietary manipulation, all groups except the normal control group received a single injection intraperitoneal (ip) of STZ (40 mg/kg b.w.) dissolved in citrate buffer (pH 4.4). One week after the STZ injection, animals with non-fasting blood glucose levels > 250 mg/dl were considered as diabetic. Four groups of STZ-induced diabetic rats were orally treated with metformin (100 mg/kg) and TVK (300, 600, and 1200 mg/kg b.w), respectively. Bodyweight recording was done weekly; fasting blood glucose levels were measured at the end of 8th week, 10th week, 12th week. Lipid profile, SGOT, SGPT,...
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Posted by admin on Mar 31, 2020 in |
The aim of this work is a comparison of anti-inflammatory properties of the molecular pectin complex with 10% acetylsalicylic acid (P-ASA) and acetylsalicylic acid (ASA) in equimolar doses on the model of carrageenan-induced paw edema of rats. The study was carried out on a total of 28 adult male Sprague Dawley rats at 7 months of age. 30 min after injection of 1% aqueous solution of carrageenan to the plantar aponeurosis, studied drugs were orally administered once at the doses of 10, 20, 40 mg/kg ASA and 100, 200, 400 mg/kg P-ASA. The severity of edema, pain sensitivity, leukocyte count, its subpopulation ratio and IL-1β, IL-6, IL-8, TNFα level were determined. Normality, t-test and Mann-Whitney tests were used for statistical analyses. The ulcerogenic effect of P-ASA (dose 400 mg/kg) is significantly less than that of ACA (dose 40 mg/kg). The analgesic effect of P-ASA is comparable to ASA at equimolar doses. The anti-edema effect of ASA at the doses of 10 and 20 mg/kg is more pronounced than that...
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Posted by admin on Mar 31, 2020 in |
Histomorphological changes were observed to assess the effect of fungus on gill and pancreas of Channa striatus. The most frequent histological changes detected in the gills included hypertrophy, hyperplasia and fusion of secondary lamellae. Other lesions found were vacuolization and blood congestion in Pancreas. The colonization of aquatic fungi in teleost fish is a severe problem affecting both wild and cultured fish population. However, the fungal infection is more abundant in the captive environment which adversely affects fish industry. An external fungal infection cause lesions, subsequently become enlarge and may lead death. Stress, physical injury, malnutrition and poor water quality increase the susceptibility of fungal infections. Gill and Pancreas are responsible for vital functions such as respiration, excretion, erythropoiesis, regulating blood pressure and the accumulation. The alterations found in these organs are easier to identify than functional ones and serve as warning signs of damage to animal health. Hence, the study of tissue deformities in respect to fungal infection which is commonly encountered in freshwater aquaculture needs to be...
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Posted by admin on Mar 31, 2020 in |
In-vitro investigations toward DNA damage reported that the biological effect of genotoxic exposures could influence the life cycles of different human cells. Sister chromatid exchange (SCE) assay is broadly used, sensitive cytogenetic biomarker of exposure to genotoxic agents. Naturally available products may play a significant role against the cytogenetic damage but available research data is not consistent. The purpose of this study was to evaluate the anti-genotoxic potential of beet root extract (BRE) against hair dye induced genotoxicity in cultured human peripheral blood lymphocytes (PBLs) using SCE assay. For this, PBLs were treated with different doses of hair dye ingredients along with BRE. Significant (p<0.05) rise in SCE frequency indicates genotoxicity of hair dye ingredients (p-Phenylenediamine-200 µg/ml, resorcinol-200 µg/ml, and hydrogen peroxide-6.168 µg/ml). Treatment of BRE along with hair dye showed significant (p<0.05) reduction in SCE frequency in a dose-dependent manner as compared to hair dye treated...
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Posted by admin on Mar 31, 2020 in |
Objective: Mycoplasma hominisis a gram-negative bacteria belonging to class Mollicutes that is commonly present in men and women of reproductive age. At present, there is no effective prophylaxis for Mycoplasma hominis, as it has gained resistance for the drugs hence an in-silico approach was undertaken to find out the peptide-based vaccine for the pathogen. Methods: In women, it affects the genital tract and involved in causing pelvic inflammatory disease, ectopic pregnancy, early delivery, miscarriage and prolonged infection may lead to infertility. M. hominis can also be transmitted from mother during childbirth and cause fever and infection in the new-born baby. All the 529 protein sequences of Mycoplasma hominis were taken in FASTA format from the UniProt proteome database. Antigenicity study was done for all the proteins of the pathogen using VaxiJen v2.0 and the proteins with high antigenicity scores were taken for further analysis like structural and functional analysis using InterPro, molecular docking using Autodock and molecular simulation study was carried out using Charmm. Results: The study identifies 50S ribosomal protein L28,...
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