Posted by admin on Jun 30, 2020 in |
The global prevalence of infectious diseases caused by microorganisms is a major public health concern worldwide. The emergence of antibiotic resistance and related toxicity issues are limiting the use of antibiotics and prompting research for alternative therapy. Plant-derived drugs are gaining popularity due to their efficacy and safety. Calotropis procera and Calotropis gigantea are two common species in the genus Calotropis, well known for their ethnomedicinal values. Present study comparatively evaluated the phytochemical screening and antimicrobial activity of C. procera and C. gigantea. The qualitative phytochemical screening of the ethanolic, methanolic, and aqueous extracts of Calotropis sp. indicated similar phyto-constituents of both the species. Secondary metabolites such as alkaloids, terpenoids, flavonoids, glycosides, cardiac glycosides, coumarin, steroid, phenol, tannin, saponin, and volatile oils were detected in both the plant extracts and ethanolic extracts contained the highest number of the plant metabolites. The plant extracts showed antimicrobial activity against Gram +ve and –ve human pathogenic bacteria, indicating their broad-spectrum activity. Comparatively, C. procera was more effective against the tested bacterial pathogens with lower MIC and...
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Posted by admin on Jun 30, 2020 in |
Plants and plants-based medicines have been used to treat many ailments since the ancient times. Family Orchidaceae to which orchids belongs is the largest family among monocotyledons. The genus Eulophia comprises perennial terrestrial orchids with fleshy tubers, rarely pseudobulbs. One such perennial herb having underground tubers, Eulophia nuda, belonging to family Orchidaceae, has been traditionally used for the treatment of tumours, scrofulous glands of the neck, bronchitis, skin rash and rheumatoid arthritis. In the present study the pharmacognostical and phytochemical evaluation of tubers of E. nuda was performed as it is helpful for the standardization and authentication of medicinal plant. The microscopical study showed the presence of xylem vessels, fibres, starch, raphides and acicular crystals. The phytochemical analysis of the tubers of E. nuda indicated the presence of carbohydrates, alkaloids, flavonoids, steroids, triterpenoids, etc. The results of the HPTLC fingerprinting confirmed the presence of the flavonoids like rutin, quercetin and kaempferol in the tubers of E....
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Posted by admin on Jun 30, 2020 in |
A simple, precise, and sensitive stability-indicating high-performance thin layer chromatographic (HPTLC) method has been developed and validated for the analysis of Darunavir in bulk and in the tablet dosage form. The separation was performed on pre-coated silica gel 60 GF254 plates using Toluene: Methanol: Triethylamine (8.5:1:0.5 v/v/v) as the mobile phase. The retention factor (Rf) was found to be 0.61 ± 0.89. The detection of a band was carried out at 267 nm. The drug was subjected to different stress conditions like acid, base hydrolysis, oxidation, thermal degradation, and photolysis. The method was successfully validated according to ICH Q2 (R1) guidelines. The linear regression analysis data for the calibration plot showed a good linear relationship with R2 = 0.992 in the range of 500-3000 ng band-1. The method found to be accurate as results of the recovery studies are close to 100%. The developed method can be adopted for routine analysis of Darunavir in bulk and pharmaceutical dosage...
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Posted by admin on Jun 30, 2020 in |
Bombax ceiba is a traditional medicinal plant that is beneficial in the treatment of diuretic, hemostatic, antidysenteric, emetic, antipyretic, astringent, and commonly known silk cotton tree, semal, kapok tree. The aim of this study is to isolate and characterize the biologically active compounds from the ethanolic extract of the leaves. The EtOH extract was subjected to silica gel (60-120 mesh) by glass column chromatography, using gradient petroleum-ether, C6H6, CH3OAc, CH3OH, and purified by preparative TLC (GF254) to yield two pure natural products (taraxerol and β-sitosterol glucoside). The structure was elucidated on the basis of spectroscopic analysis, including 1HNMR, 13CNMR, DEPT-135, COSY, HSQC, and HMBC. The β-sitosterol glucoside shows pharmacological activities such as lowering cholesterol levels, reducing blood pressure, antitumor activity, etc. The glucose moiety is highly effective because it is hydrophilic, which inhibits the entry of cholesterol into the esterification of cholesterol. It is the first time isolated from the leaves of this...
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Posted by admin on Jun 30, 2020 in |
Objective: The present study is designed to investigate vasorelaxation mechanisms of Nanocurcumin in the middle uterine artery (MUA) of both non-pregnant (NP) and pregnant (P) Capra hircus (Ch). Methods: MUA rings were mounted in an automatic organ bath and the contractile response was recorded isometrically by highly sensitive force transducer connected to a power lab data acquisition system. Nanocurcumin (1 pM-100 µM) vasorelaxation response (NCVR) was elicited in PE-precontracted rings either in the absence or presence of different blockers. Results: The maximal vasorelaxation (Rmax) to Nanocurcumin in phenylephrine-precontracted endothelium intact MUA rings of NP (40.36 ± 2.38%) and of P (44.09 ± 3.41%) was reduced to 22.93 ± 0.80% and 25.37 ± 2.2% in endothelium-denuded MUA rings of NP and P Ch. L-NAME, Indomethacin and L-NAME + Indomethacin inhibited NCVR by significantly decreasing the Rmax to 17.65%, 28.42%, 17.95% in NP and to 27.34%, 39.06%, 24.50% in P Ch. The ODQ and carbenoxolone/18β Glycyrrhetinic acid did not inhibit the Rmax of NCVR. Conclusion: Nanocurcumin potentially dilates the MUA of...
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