Posted by admin on Mar 31, 2020 in |
A combination of Grazoprevir and Elbasvir is used to treat Hepatitis C virus (HCV). A selective, accurate and precise RP-HPLC method was developed and validated for the simultaneous estimation of these drugs in combined tablet dosage forms. The drugs were resolved on a BDS C18 column using 0.1% orthophosphoric acid: Acetonitrile (45:55 v/v) as the mobile phase. The detection wavelength was 260 nm. The retention times obtained for Grazoprevir and Elbasvir were 2.400 & 3.018 min respectively. The linearity ranges were 25-150 & 12.5-75µg/ml respectively with Regression coefficients of 0.999. The % R.S.D. of precision studies was found to be 0.6 & 0.4 respectively. The accuracy of the proposed method was determined by recovery studies and the mean recovery was 99.14 & 100.34%, respectively. The method was also applicable for quantitative analyses of the marketed tablet formulations and in studying the stability of the drugs under acidic, alkaline, oxidation, thermal and UV...
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Posted by admin on Mar 31, 2020 in |
α-amylase is widely used in various biotechnological applications such as food processing, starch, and paper industries, hydrolyzing starch, glycogen, and other polysaccharides into glucose, maltose, and oligosaccharides. Aspergillus niger α-amylase was partially purified by ammonium sulphate fractionation with 1.409 fold purity and 54.88% yield. The partially purified α-amylase was immobilized on sodium alginate with calcium chloride by ionotropic gelation with glutaraldehyde as a cross-linking agent. Immobilized α-amylase exhibited 50% of its original activity. The soluble and immobilized α-amylases exhibited maximum activity at pH values 7.5 and 8.0, respectively. The optimum temperature for both the soluble and immobilized enzymes was 35 ºC. The immobilized enzyme was more pH and thermally stable than the soluble one. The substrate starch was hydrolyzed by soluble (Km 0.6 mg/ml, Vmax 16.05 mg/ml/min) and immobilized α-amylase (Km 0.65 mg/ml, Vmax 17.41 mg/ml/min) with high efficiencies. On the basis of the results obtained, immobilized α-amylase could be employed in the saccharification of starch...
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Posted by admin on Mar 31, 2020 in |
Globally, the outbreak of antibiotic-resistant pathogens derived infections urged an intensifying necessity for the survey of underexplored marine zone microorganisms for novel metabolites. Our current study emphasized on isolation, polyphasic characterization and bioactivity of the novel actinomycetes from the Bay of Bengal near Kakinada and Nizampatnam, Andhra Pradesh, India, which could provide lead bioactive compounds of therapeutic efficacy to repress the of pervasiveness multidrug-resistant pathogens. A total of fifteen actinobacterial strains (VJRM-1 to VJRM-15) were isolated and tested. Micro-morphological characteristics of the isolates were perceived by SEM (scanning electron microscope) analysis. Phylogenetic analyses of isolates were appraised with 16S rRNA gene sequence homology. The dendrogram was contrived by deploying the parsimony method in MEGA software version 6. Based on polyphasic studies, strains VJRM-7 and VJRM-8 identified as Nocardiopsis dassonvillei (KP299158) and Nocardiopsis sp (KP299159). In-vitro antimicrobial activity of crude ethyl acetate extracts derived from these strains revealed strong antagonistic activity against Gram-positive, Gram-negative bacteria and fungi. Both strains displayed positive results for the production of enzymatic activities. The morpho-physio-molecular...
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Posted by admin on Mar 31, 2020 in |
Objective: Present research work describes an eco-friendly and quick method for the development of silver nanoparticles (AgNP) using Momordica charantia Linn. extract and silver nitrate solution. Our investigation reports the antimicrobial activity of developed nanoparticles against Staphylococcus aureus, Escherichia coli. Method: Silver nanoparticle developed by reduction of silver nitrate solution by Momordica charantia Linn. extract. Extract of Momordica charantia Linn. acts as a reducing agent. Ulta Violet Visible spectroscopy used as a confirmatory test for the development of silver nanoparticles. Result: The silver nanoparticles synthesized were confirmed by their change of color to dark brown due to the phenomenon of surface plasmon resonance. The characterization studied was done by UV-visible spectroscopy, Scanning electron microscopy (SEM), X-Ray diffraction (XRD), Fourier Transmission infrared spectroscopy (FTIR), and study antibacterial activity. FTIR gives information about functional groups present in the synthesized silver nanoparticles. X-ray diffraction studies indicate that developed silver nanoparticles are highly crystalline in nature. SEM of synthesized AgNP shows that particles are spherical in shape and uniformly distributed. Synthesized AgNP shows...
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Posted by admin on Mar 31, 2020 in |
The objective of the research was to investigate and evaluate anti diarrheal, antioxidant activity Mimusops elengi Linn. unripen fruit extracts. Mimusops elengi Linn. unripen fruits is considered as one of the best medicinal plants due to its several pharmacological uses mentioned in Unani as well as ethnomedicine. The various extracts of the plant (bark, fruit, leaves, seed, and flowers) have been disclosed to be cardiotonic, alexipharmic and stomachic, hypotensive, antibacterial, anthelmintic, anti gastric ulcers, teeth cleaner and renewable sources of energy. This review is an attempt to compile and document information on conventional uses and phytochemical properties of Mimusops elengi Linn. unripen fruits. The antidiarrhoeal activity of pet ether, chloroform and methanol extracts of Mimusops elengi unripen fruits were assessed using castor oil-induced diarrhea model in rats. Further, the effects of pet ether, chloroform, and methanol extract on gastrointestinal tract motility after charcoal meal dispensed were evaluated. Loperamide was taken as positive control. The plant extracts showed decisive and significant (P<0.05) thwarting action against castor oil-induced diarrhea in rats...
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