Posted by admin on Mar 31, 2020 in |
Scientific investigations revealed that the study of P2Y1 receptors is very much essential in the current scenario because of their potential role in related to various disorders/diseases like thrombosis, cardiovascular problems. P2Y1 receptors belong to G protein-coupled receptors, an important target for ADP induced platelet aggregation. Blockade of P2Y1 receptors leads to the treatment of thrombosis with a potentially improved safe margin. Hence, it is essential to select targeted molecules as P2Y1 receptor antagonists. The present work is to explore P2Y1 receptor antagonists from different series of synthetic compounds by using docking, virtual screening, and toxicity studies. Docking studies were performed and scored to evaluate ligand binding affinities. The present work could be used as a tool to show how different scaffold structures are utilized for the development of suitable P2Y1 receptor antagonists for platelet aggregation...
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Posted by admin on Mar 31, 2020 in |
Lornoxicam has been found to be not interacted by eperisone hydrochloride at 377 nm. Therefore, it is estimated directly as a single component in the mixture, whereas eperisone hydrochloride is throughout interfered with by lornoxicam in UV region. So, it was estimated in the mixture using a two-wavelength difference method. Lornoxicam was found to have same absorbance at 262 nm and 283 nm but not eperisone hydrochloride. So, a difference in absorbance of the mixture at 262 nm and 283 nm was directly proportional to the concentration of eperisone hydrochloride. The calibration curves were validated according to ICH guidelines and were linear over the concentration range tested (10-20 µg/ml for lonoxicam and 3-24 µg/ml for eperisone hydrochloride). The curves were found to be precise and accurate as shown by acceptable limits of %RSD (0.37%-0.66%) and percent recoveries (98.73% ± 1.02%-102.44% ± 0.90%) respectively for both the drugs in mixture as well as in pure...
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Posted by admin on Mar 31, 2020 in |
The purpose of this study was to develop a gastroretentive controlled release drug delivery system using sintering for enhanced oral bioavailability using carnauba wax polymer. Formulations (CC1-CC3) were prepared by wet granulation method and then exposed to sintering temperature at different time intervals. The optimized formula showed zero-order drug release kinetics for the period of 12 h. The results revealed that sintering temperature and time of exposure significantly affected the in-vitro drug release and floating character. FTIR Spectra showed no noticeable incompatibility between drug and polymers in both physical mixtures and in the...
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Posted by admin on Mar 31, 2020 in |
HPTLC is a simple, rapid, precise and accurate method with densitometric detection for the determination of beta-sitosterol in a Pergularia daemia Forsk. Petroleum ether and methanol extract of the leaf and stem were powder and used for the experimental work. The separation was performed on silica gel 60 F254 HPTLC plates with Toluene: Ethyl acetate (80:20 v/v), as the mobile phase. The determination was carried out using the densitometric absorbance mode at 690 nm. β-sitosterol response was linear over the range of 100-1000µg/ml, with a correlation coefficient is 0.999. Intraday and interday precision studies showed the relative SD was 0.027. The accuracy of the method was determined, and the average recovery was 99.92%.The limit of quantitation and limit of detection were 8 and 4 mg/ml, respectively. The percentage of beta-sitosterol was found to be 22.16% w/w in unsaponifiable matter of leaf, 33.05% unsaponifiable matter of stem w/w 2.49% w/w petroleum ether extract of leaf 3.28% w/w petroleum ether extract of stem. The method was evaluated for sensitivity, accuracy, precision,...
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Posted by admin on Mar 31, 2020 in |
Free radicals are responsible for the development of many diseases. Anti-oxidants play an important role in scavenging free radicals. Anti-oxidant activity of herbal extracts and isolated compounds are reported earlier. Pharmaceutical industries are carrying out research in the development of new potent antioxidants. Novel drug development from economically cheapest plant waste might be useful for many pharmaceutical industries. Tender coconut mesocarp (TCM) is one of the cheapest plant materials used by coir industry etc. Aim of the present study is to analyses various phytochemicals present in the hydroalcoholic extract of TCM and evaluate its antioxidant activity in the in-vitro condition. Qualitative analysis of phytoconstituents present in hydroalcoholic extract (HAE) of TCM shows the presence of carbohydrate, phenolic compounds, flavonoids and tannin. Quantitative analysis of phytoconstituents in HAE shows the remarkable amount of phenols, flavonoids, tannin and vitamin C. LCMS analysis of HAE shows the presence of (±) 13-Azaprostanoic acid with maximum ppm (13.58) and 14 other compounds with ppm > 3n were identified. The IC50 value of HAE to...
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