Posted by admin on Mar 31, 2020 in |
The objective of the present study was characterization, production, and purification of Nattokinase from Bacillus subtilis. A fibrinolytic enzyme-producing bacterium was isolated and identified as Bacillus subtilis from fermented soybean and soil. Bacillus subtilis showed a very strong protease activity by degrading casein skimmed milk agar. Nattokinase was produced on basal medium and then purified from supernatant. The crude enzyme was purified by ammonium sulphate precipitation, followed by dialysis and DEAE cellulose ion-exchange chromatography. Total protein was estimated by Lowry’s method (0.58 mg/ml for FS2 and 0.49 mg/ml for S1) and enzyme-specific activity was determined by tyrosine standard (0.52 µmole/mg/ml for S1 and 0.59 µmole/mg/ml for FS2). The molecular weight of purified enzyme was determined by SDS-PAGE (20-24 kDa for FS2 and 41-44 kDa for S1). Finally, the fibrinolytic activity was determined by application of extracted enzyme on pre-existing human blood clots (FS2 showed greater activity compared to...
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Posted by admin on Mar 31, 2020 in |
Environment provides a solid platform for good health. An unhealthy environment makes place unsafe for wild animals. One of the effects is a parasitic load on these animals, as a high degree of parasitization may cause harm to the health of these animals. In the present research work, blood parasitic infestation in two species of lizards, Hemidactylus flaviviridis and Calotes versicolor were investigated and it was observed that lizards from the highly polluted area were infested with high parasite load that ultimately caused harm to these animals which biologically constitute an important part of the food chain. The intensity of blood parasites (Hepatozoan and Plasmodium) in lizards from different habitats was studied and it was observed that highly polluted areas (Mirzapur) had a high incidence of the parasites. It is known that Mirzapur is more polluted than Bareilly and Chandausi, the other sites selected for investigation. Parasites are regarded as indicators of pollution. To make the environment healthy and peaceful, positive energy and healthy habitats can contribute to a...
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Posted by admin on Mar 31, 2020 in |
The present study aimed to investigate the combined anticonvulsant effect of nifedipine (calcium channel blocker) and pentazocine (opioid analgesic) on the duration of convulsions, tonic hind limb extension and recovery in mice and rats. The study was initiated after obtaining ethical approval from the Institutional Animal Ethics Committee (IAEC), Department of Pharmacology, Osmania Medical College, Koti, Hyderabad. The anticonvulsant effect of these drugs were screened using Maximal Electro-Shock (MES) method and animals showing tonic hind limb extension response were divided into groups (six animals per group) in both species. Both mice and rats (6 animals/group) were treated with nifedipine (10 mg/kg), pentazocine (30 mg/kg), a combination of nifedipine (10 mg/kg) and pentazocine (30 mg/kg) and control animals are given distilled water as vehicle. The drug administered by an intraperitoneal route. The data were analyzed using ANOVA and group means were compared with LSD Post Hoc Test. P values < 0.05 were considered as significant. The animals treated with both nifedipine 10 mg/kg and pentazocine 30 mg/kg showed a significant...
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Posted by admin on Mar 31, 2020 in |
The main of this study was production of floating tablets to maintain the drug stay in its absorption area. Ciprofloxacin is better absorbed in the stomach because of this reason floating matrix tablet was prepared using novel sintering technique with sodium bicarbonate as gas generating agent and a polymer eudragit robust formulation was decided on the basis of floating time and in-vitro drug release with possible less polymer concentration, time of exposure and at low temperature. Three batches were prepared with hardness between 4-6 kg/cm2 based on time of exposure at particular sintering temperature desired total floating time, and drug release was retarded up to 12 h. Hence, it is evident from this investigation that a floating matrix tablet using the sintering technique could be promising delivery with improved sustained release action and drug...
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Posted by admin on Mar 31, 2020 in |
A combination of Grazoprevir and Elbasvir is used to treat Hepatitis C virus (HCV). A selective, accurate and precise RP-HPLC method was developed and validated for the simultaneous estimation of these drugs in combined tablet dosage forms. The drugs were resolved on a BDS C18 column using 0.1% orthophosphoric acid: Acetonitrile (45:55 v/v) as the mobile phase. The detection wavelength was 260 nm. The retention times obtained for Grazoprevir and Elbasvir were 2.400 & 3.018 min respectively. The linearity ranges were 25-150 & 12.5-75µg/ml respectively with Regression coefficients of 0.999. The % R.S.D. of precision studies was found to be 0.6 & 0.4 respectively. The accuracy of the proposed method was determined by recovery studies and the mean recovery was 99.14 & 100.34%, respectively. The method was also applicable for quantitative analyses of the marketed tablet formulations and in studying the stability of the drugs under acidic, alkaline, oxidation, thermal and UV...
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