Posted by admin on Feb 29, 2020 in |
Delayed wound healing is producing a high economic burden on the society and is generally associated with Diabetes mellitus or a related complication thus needs safe treatment like herbal formulation. Dermas heal cream (an anti-diabetic ayurvedic formulation) was evaluated for wound and burn healing potential in normal and diabetic rats. The effect of Derma heal cream on wound healing has been studied in diabetic and normal animals and compared with standard using the excision wound model. Diabetes was induced by a single dose of Streptozotocin 55 mg/kg i.p. Rats with blood glucose levels >250 mg/dl are considered as diabetic and included in the study for incision, excision and burn heal model. Rats treated with derma heal cream (DHC) at doses 1000 mg/kg dose per day were applied topically in the excision wound model, treated DHC shows significant effect reduction in period of 21st day and wound area is (5 ± 1.15 mm)and normal group wound area is (1.66 ± 2.12 mm) as compared to diabetic control group (25 ±...
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Posted by admin on Feb 29, 2020 in |
The present study was carried out to evaluate phytochemical composition and pharmacological activities of leaf extract of Lusia zeylanica by using different solvents. The solvents like n-hexane, ethyl acetate and methanolic were used to extract dried leaf material of L. zeylanica. These extracts were analyzed for phytochemical constituents with GC-MS analysis and antimicrobial activity tested against four bacterial strains and three fungal strains by using the agar diffusion method. In-vitro anticancer activity was done against two cancer cell lines (MCF-7 and HeLa cell line) by using MTT assay. Phytochemical analysis revealed the presence of coumarins, flavonoids, glycosides, phenols, saponins, tannins, and terpenoids. GC-MS analysis determines the presence of 11 compounds in ethyl acetate, 7 compounds in methanolic extracts and a total of 7 unknown compounds respectively. A significant cancer cell growth inhibition was observed for two extracts with IC50 values ranging between 18.36 µg/ml to 67.914 µg/ml. Our result shows this plant is a promising source of phytocompounds with potential antimicrobial and anticancer...
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Posted by admin on Feb 29, 2020 in |
Acute lymphoblastic leukaemia (ALL) of the B-cell lineage (B-ALL) is a malignant neoplasm identified by the accumulation of immature lymphoid progenitor cells in bone marrow and blood. Numerous chromosomal aberrations have been found to be correlated with disease, relapse and its clinical outcome. The survival and cure rate has been improved drastically with the advent of modern methods of diagnosis and availability of targeted pharmacological agents. However, the limitation is the higher incidence of relapsed disease. An 8-year old male child was diagnosed with B-ALL, the disease relapsed despite proper therapeutic regimen after reporting to the hospital. Conventional cytogenetic analysis revealed a normal male karyotype. FISH panel for B-ALL including t(9;22) BCR-ABL, t(12;21) ETV6-RUNX1, KMT2A (11q23) rearrangement and t(1;19) PBX1-E2A was performed and was positive for translocation t(12;21) ETV6-RUNX1 in 70% of the cells at the time of diagnosis. At the time of relapse, patient underwent cytogenetics workup including cytogenetics and FISH. Bone Marrow karyotyping showed a male karyotype with t(12;21) in all the metaphases studied and FISH was...
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Posted by admin on Feb 29, 2020 in |
Background: Berberine is a natural alkaloid used as traditional Chinese medicine. Objective: The objective of the present study was to evaluate the effect of berberine on dibutyltin dichloride (DBTC) induced acute pancreatitis in albino Wistar rats. Methods: In this study 24 Wistar rats were randomly selected and divided into four groups. Each group consists of 6 animals. Group-1 (-ve control), Group 2 (+ve control or disease control), Group 3 (Berberine 50 mg/kg p.o), Group 4 (Berberine 100 mg/kg, p.o). Acute pancreatitis was induced with dibutyltin dichloride at a single dose of 6mg/kg body weight i.p to all the groups except negative control. At 3rd day, serum amylase and lipase levels were elevated in all groups except negative control meanwhile rats were treated with berberine up to 28 days. The estimation of blood parameters and tissue parameters was carried out by the auto-analyzer. Results: Berberine treated groups significantly reduce elevated serum parameters, and significantly increase the tissue antioxidant parameters to normal. Serum parameters like serum amylase, lipase, fasting blood glucose...
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Posted by admin on Feb 29, 2020 in |
Objective: Nanoliposomes of letrozole a drug used for breast cancer treatment were formulated. The present investigation was aimed to optimize the phospholipids concentration by formulating a series of nanoliposomes formulation and their characterization. Methods: Nanoliposomes were prepared using reverse-phase evaporation technique employing diverse ratios of soya lecithin and cholesterol concentration. The effect of various factors was studied on size, polydispersity index, loading capacity and entrapment efficiency of nanoliposomes. The prepared nanoliposomes were analyzed using DSC and ATR spectroscopy for drug-excipients compatibility. Results: The smallest particle size and polydispersity index (99.45, 0.262) highest entrapment (87.24 ± 0.59) and loading capacity (56.89 ± 0.39) was observed in batch NL8. The disappearance of drug peak in DSC indicates the dissolution of the drug in a polymer matrix. The ATR spectroscopy results show there was no interaction between components and drug is stable in the formulated nanoliposomes. Conclusion: It is concluded that the concentration of soya lecithin and cholesterol were critical parameters that affect the encapsulation of letrozole in the formulation and optimization...
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