Posted by admin on Mar 31, 2020 in |
α-amylase is widely used in various biotechnological applications such as food processing, starch, and paper industries, hydrolyzing starch, glycogen, and other polysaccharides into glucose, maltose, and oligosaccharides. Aspergillus niger α-amylase was partially purified by ammonium sulphate fractionation with 1.409 fold purity and 54.88% yield. The partially purified α-amylase was immobilized on sodium alginate with calcium chloride by ionotropic gelation with glutaraldehyde as a cross-linking agent. Immobilized α-amylase exhibited 50% of its original activity. The soluble and immobilized α-amylases exhibited maximum activity at pH values 7.5 and 8.0, respectively. The optimum temperature for both the soluble and immobilized enzymes was 35 ºC. The immobilized enzyme was more pH and thermally stable than the soluble one. The substrate starch was hydrolyzed by soluble (Km 0.6 mg/ml, Vmax 16.05 mg/ml/min) and immobilized α-amylase (Km 0.65 mg/ml, Vmax 17.41 mg/ml/min) with high efficiencies. On the basis of the results obtained, immobilized α-amylase could be employed in the saccharification of starch...
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Posted by admin on Mar 31, 2020 in |
Globally, the outbreak of antibiotic-resistant pathogens derived infections urged an intensifying necessity for the survey of underexplored marine zone microorganisms for novel metabolites. Our current study emphasized on isolation, polyphasic characterization and bioactivity of the novel actinomycetes from the Bay of Bengal near Kakinada and Nizampatnam, Andhra Pradesh, India, which could provide lead bioactive compounds of therapeutic efficacy to repress the of pervasiveness multidrug-resistant pathogens. A total of fifteen actinobacterial strains (VJRM-1 to VJRM-15) were isolated and tested. Micro-morphological characteristics of the isolates were perceived by SEM (scanning electron microscope) analysis. Phylogenetic analyses of isolates were appraised with 16S rRNA gene sequence homology. The dendrogram was contrived by deploying the parsimony method in MEGA software version 6. Based on polyphasic studies, strains VJRM-7 and VJRM-8 identified as Nocardiopsis dassonvillei (KP299158) and Nocardiopsis sp (KP299159). In-vitro antimicrobial activity of crude ethyl acetate extracts derived from these strains revealed strong antagonistic activity against Gram-positive, Gram-negative bacteria and fungi. Both strains displayed positive results for the production of enzymatic activities. The morpho-physio-molecular...
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Posted by admin on Mar 31, 2020 in |
Objective: Present research work describes an eco-friendly and quick method for the development of silver nanoparticles (AgNP) using Momordica charantia Linn. extract and silver nitrate solution. Our investigation reports the antimicrobial activity of developed nanoparticles against Staphylococcus aureus, Escherichia coli. Method: Silver nanoparticle developed by reduction of silver nitrate solution by Momordica charantia Linn. extract. Extract of Momordica charantia Linn. acts as a reducing agent. Ulta Violet Visible spectroscopy used as a confirmatory test for the development of silver nanoparticles. Result: The silver nanoparticles synthesized were confirmed by their change of color to dark brown due to the phenomenon of surface plasmon resonance. The characterization studied was done by UV-visible spectroscopy, Scanning electron microscopy (SEM), X-Ray diffraction (XRD), Fourier Transmission infrared spectroscopy (FTIR), and study antibacterial activity. FTIR gives information about functional groups present in the synthesized silver nanoparticles. X-ray diffraction studies indicate that developed silver nanoparticles are highly crystalline in nature. SEM of synthesized AgNP shows that particles are spherical in shape and uniformly distributed. Synthesized AgNP shows...
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Posted by admin on Mar 31, 2020 in |
The objective of the research was to investigate and evaluate anti diarrheal, antioxidant activity Mimusops elengi Linn. unripen fruit extracts. Mimusops elengi Linn. unripen fruits is considered as one of the best medicinal plants due to its several pharmacological uses mentioned in Unani as well as ethnomedicine. The various extracts of the plant (bark, fruit, leaves, seed, and flowers) have been disclosed to be cardiotonic, alexipharmic and stomachic, hypotensive, antibacterial, anthelmintic, anti gastric ulcers, teeth cleaner and renewable sources of energy. This review is an attempt to compile and document information on conventional uses and phytochemical properties of Mimusops elengi Linn. unripen fruits. The antidiarrhoeal activity of pet ether, chloroform and methanol extracts of Mimusops elengi unripen fruits were assessed using castor oil-induced diarrhea model in rats. Further, the effects of pet ether, chloroform, and methanol extract on gastrointestinal tract motility after charcoal meal dispensed were evaluated. Loperamide was taken as positive control. The plant extracts showed decisive and significant (P<0.05) thwarting action against castor oil-induced diarrhea in rats...
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Posted by admin on Mar 31, 2020 in |
Scientific investigations revealed that the study of P2Y1 receptors is very much essential in the current scenario because of their potential role in related to various disorders/diseases like thrombosis, cardiovascular problems. P2Y1 receptors belong to G protein-coupled receptors, an important target for ADP induced platelet aggregation. Blockade of P2Y1 receptors leads to the treatment of thrombosis with a potentially improved safe margin. Hence, it is essential to select targeted molecules as P2Y1 receptor antagonists. The present work is to explore P2Y1 receptor antagonists from different series of synthetic compounds by using docking, virtual screening, and toxicity studies. Docking studies were performed and scored to evaluate ligand binding affinities. The present work could be used as a tool to show how different scaffold structures are utilized for the development of suitable P2Y1 receptor antagonists for platelet aggregation...
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