Posted by admin on Jan 31, 2020 in |
Bacteria have proved remarkably developing resistant to a known antibiotic; however, Medicinal plants have manifested notably useful for the control of bacterial infections. This study aimed to isolate and identify the multidrug-resistant bacteria from wound infection, then examine the antibacterial activity of different concentration of the ethanolic extracts of Cymbopogon proximus, Azadirachta indica, and Grewia senegalensis plants against it. One hundred specimens collected from wound infection, isolation and identification done according to standard methods. Antibiotics susceptibility test performed using the Kirby-Bauer disc diffusion method for: Cotrimoxazole (25 μg) Ciprofloxacin (5 μg), Gentamicin (10 μg), Imipenem (10 μg), Erythromycin (15 μg), Tetracycline (30 μg) Methacillin (5 μg), Ceftazidime (30 μg). 94 isolates showed bacterial growth, 40 (42.6%) isolates were Gram-positive bacteria (Staphylococcus aureus) and 54 sample Gram-negative bacteria; 15 (27.8%) Pseudomonas aeruginosa, 13 (24.1%) Proteus spp, 12 (22.2%) Escherichia coli, 10 (18.5%) Klebsiella pneumonia, 4 (7.4%) Citrobacter freundii. Ethanolic extracts from Sudanese medicinal plants namely Cymbopogon proximus, Azadirachta indica, and Grewia senegalensis, screened for their antimicrobial properties against isolated multidrug-resistant...
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Posted by admin on Jan 31, 2020 in |
Quercetin is a plant flavonol that is available from both daily diet and nutraceuticals. Various studies report that Quercetin alters the pharmacokinetics of various drugs by mechanisms like p-glycoprotein (p-gp) inhibition or other unknown mechanisms. The study was undertaken to evaluate the effect of Quercetin on the pharmacokinetics of Eletriptan. A single dose in-vivo pharmacokinetic study was carried out in rat models. In this study, rats were treated with Quercetin (10 mg/kg) and Eletriptan (2 mg/kg) orally and blood samples were collected at various time points such as 0 (predose), 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 6.0, 8.0, 12, 24 h post-treatment. Plasma concentration of Eletriptan was estimated using the HPLC method. AUC (0-24) of Eletriptan has significantly (p<0.01) increased in the Eletriptan and Quercetin combination group 1451.98 ± 93.78*** (ng/ml/h) when compared to AUC (0-24) Eletriptan alone treated group 883.94 ± 77.25 (ng/ml/h). AUC0-∞ of Eletriptan has significantly (***p<0.001) increased in the combination group (1543.32 ± 182.39*** (ng/ml/h).) in comparison to AUC0-∞ of Eletriptan of Eletriptan...
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Posted by admin on Jan 31, 2020 in |
Objective: In the present dissertation work, the aim was to prepare self-emulsifying drug delivery systems (SEDDS) of mirtazapine to improve its solubility with a view to enhance its oral bioavailability. Methods: The prepared SEDDS was the concentrate of the drug, oil-Capmule MCM EP, surfactant tween 80 and PEG 400 as a co-surfactant. The optimized microemulsion was converted into solid form by adsorption technique by using as Neusiline US2 solid carrier. The formulation was evaluated for various tests such as solubility, drug content, emulsification time, phase separation study, dispersibility test, droplet size analysis and PDI determination and zeta potential and in-vitro release study, and in-vitro permeation study. Results: The optimized formulation F5 showed drug release (96.97 ± 1.89), droplet size (153), Zeta potential (-30mv). All formulations of mirtazapine SEDDS were showed faster dissolution than plain drug, mean bioavailability of mirtazapine increase in respect to the plain drug. The F5 can be further used for the preparation of various solid SEDDS. In conclusion, self-emulsifying drug delivery system has become promising tool...
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Posted by admin on Jan 31, 2020 in |
Pectin is a complex polysaccharide and has been used in food and pharmaceutical industries. The current study aimed to determine antioxidant and cytotoxic activities of pectin from peels of grape fruit (GP) and lemon (LP) by ferric reducing power (FRP) assay and brine shrimp lethality bioassay respectively. In addition, antioxidant and cytotoxic activities of GP and LP were compared with food-grade pectin (FGP). GP and LP exhibited positive results of antioxidant and cytotoxic activities. The percentage FRP of GP at 10 to 100 µg/ml was found to be higher as compared to FGP while LP showed closed values to that of FGP. The observed percentage mortality of shrimps at 10 to 1000 µg/ml by LP was found to be higher as compared to percentage mortality caused by FGP. Percentage mortality of shrimps by GP and FGP showed closed values. The LC50 of LP was found to be lower than that of FGP but close to the LC50 of standard drug etoposide. The LC50 of GP was closed to that...
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Posted by admin on Jan 31, 2020 in |
A novel copper (II) complex has been synthesized with Schiff base ligand obtained by the reaction of substituted carbohydrazone and thiosemicarbazide hydrochloride from the condensation reaction of ester and primary aromatic amine. The synthesized ligand and its copper (II) complex were characterized by various spectroscopic techniques like FT-IR, H1 NMR, UV-Visible, Fluorescence, powder XRD and Thermo-gravimetric analysis. Ligand and its copper complex were also screened for antioxidant and anticancer activities. In Cu (II) complex 1:2 copper to ligand molar ratio was obtained from analytical data. The molar conductance data confirm that complex is non- electrolytic in nature. Based on the electronic and magnetic data, a distorted octahedral geometry is ascribed for the Cu (II) complex. Ligand and its Cu (II) complex showed considerable anticancer activity against MCF-7 cell lines and more pronounced antioxidant activity in the presence of DPPH. Furthermore, molecular docking of ligand and its copper complex into the binding site of topoisomerase IIα was carried out using Auto Dock Vina software which demonstrated significant binding energies of...
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