Posted by admin on Jan 31, 2020 in |
A plethora of herbs have been tested for their activity against ulcerative colitis, a debilitating disease in the group of IBDs. However, a promising adjuvant to conventional therapy remains still at large. Ellagic acid, a polyphenol widely available in berries have shown efficacy against ulcerative colitis in preclinical models. Owing to its high content of ellagic acid and its analogs, the stem bark of Terminalia tomentosa was chosen for our study. Effect of Terminalia tomentosa hydroalcoholic extract and its ellagic acid-rich fraction were tested for their anti-colitic potential in DSS (5%) induced ulcerative colitis in mice. The evaluation was carried out for assessing the severity of disease at macroscopic biochemical and histopathological level. Myeloperoxidase and superoxide anions, an inflammatory and an oxidative marker respectively were also evaluated in all the groups. Amongst all, the highest significance was observed in the group treated with an ellagic acid-rich fraction (200 mg/kg). The ameliorative effect has been attributed to the high antioxidant potential of the...
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Posted by admin on Jan 31, 2020 in |
A stability-indicating UHPLC method was developed for the simultaneous estimation of Sofosbuvir (SOF) and Ledipasvir (LPS) in bulk and tablet dosage form. The study was performed using C18 BEH column (210 mm × 50 mm, 1.7 µm), with a mobile phase consisting of acetonitrile (55% v/v) and phosphate buffer of pH 3 (45% v/v) at a flow rate of 0.3 ml/min. The detection was carried at 247 nm using PDA detector and the retention times were found to be 0.7 and 1.2 min for SOF and LPS respectively. The developed method was validated according to ICH guidelines and the results were statistically validated. The method was found to be linear in the concentration range of 80 – 240 µg/ml for SOF with r2 value 0.999 and 18 – 54 µg/ml for LPS with r2 value 0.999. The % RSD value for precision, measured as repeatability and intermediate precision was less than 2 for both the analytes. The recovery percentages were found to be 100.13 and 99.93 for SOF and...
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Posted by admin on Jan 31, 2020 in |
Acne vulgaris is a commonly diagnosed inflammatory skin condition that affects pediatric, young and adult patients. The object of the paper is formulation development and evaluation of isotretinoin gel for the treatment of Acne vulgaris. The isotretinoin based anti-acne gel was formulated using different polymers includes carbopol 940, carbopol 934 and carbopol 980. Disodium edetate is used as chelating agents. The anti-acne gel was evaluated for solubility, drug release, pH and drug interaction, in-vitro drug release through Franz diffusion cells, acute skin irritation test and antibacterial test. The evaluation test was also compared with the marketed formulation of isotretinoin gel, that is, sortet gel. The antibacterial and anti-acne activity of different formulations was determined by the modified agar well diffusion method in the culture of Propionibacterium acne. The optimized formulation (F-IB) showed the highest spreadability (48.623 g/cm3), in all formulations and also have a high percentage of drug contents (97.2%). In–vitro diffusion study suggested that F-IA, F-IC, F-IIA and F-IIB showed more diffusion, and drug release from all the...
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Posted by admin on Jan 31, 2020 in |
Large-leaf gentian herb is a promising source of biologically active substances, which is actively used in the traditional medicine of many countries. It contains flavonoids, iridoids, secoiridoids, polysaccharides, xanthones with a wide spectrum of pharmacological activity. The aim of the research was to establish a procedure for identification of large-leaved gentian (Gentiana macrophylla Pall.) using thin-layer chromatography (TLC). In order to identify both hydrophilic and hydrophobic compounds in extracts from the plant two mobile phases were proposed which allow distinguishing specific zones by their color and relative position. Validation of developed procedure was carried out in terms of specificity and chromatographic system suitability. The specificity of the procedure was assessed by matching of TLC profiles for all studied samples. It was found that the main zones on the chromatograms are matching. Thus, chromatographic characteristics of large leaf gentian aerial parts were established for the first time using validated TLC procedure. The results of the study can be used for standardization of large-leaved gentian...
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Posted by admin on Jan 31, 2020 in |
Introduction: Medicinal plants have always played a major role in primary health care system since time immemorial. Sacred natural sites is the prime place to encompass large medicinal plant with wide range of natural resources. It potentially conserves plant biodiversity with inclusion of sacred places and sacred water bodies. Apart from social and religious importance it, regulate air and water quality of environment. The purpose of this study was to document the floristic diversity and traditional approaches towards the ethnomedicinal plants of Sacred Natural Sites of Panchkoshi Pilgrimage of Varanasi district, Uttar Pradesh India. Methods: An ethnobotanical survey was conducted from May 2016 to April 2018. The information collected through open-ended and semi-structured questionnaire and data were analyzed through Informant consensus factor (ICF). Results: During the field study, 66 medicinal plant species belonging to 62 genera and 36 families used to heal different illnesses were recorded. Fabaceae has the dominant family with 8 plant species and Apocynaceae and Moraceae with 5 plant species followed by Malvaceae, Poaceae, Solanaceae, and...
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