Posted by admin on Mar 31, 2020 in |
The present study aimed to investigate the combined anticonvulsant effect of nifedipine (calcium channel blocker) and pentazocine (opioid analgesic) on the duration of convulsions, tonic hind limb extension and recovery in mice and rats. The study was initiated after obtaining ethical approval from the Institutional Animal Ethics Committee (IAEC), Department of Pharmacology, Osmania Medical College, Koti, Hyderabad. The anticonvulsant effect of these drugs were screened using Maximal Electro-Shock (MES) method and animals showing tonic hind limb extension response were divided into groups (six animals per group) in both species. Both mice and rats (6 animals/group) were treated with nifedipine (10 mg/kg), pentazocine (30 mg/kg), a combination of nifedipine (10 mg/kg) and pentazocine (30 mg/kg) and control animals are given distilled water as vehicle. The drug administered by an intraperitoneal route. The data were analyzed using ANOVA and group means were compared with LSD Post Hoc Test. P values < 0.05 were considered as significant. The animals treated with both nifedipine 10 mg/kg and pentazocine 30 mg/kg showed a significant...
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Posted by admin on Mar 31, 2020 in |
The main of this study was production of floating tablets to maintain the drug stay in its absorption area. Ciprofloxacin is better absorbed in the stomach because of this reason floating matrix tablet was prepared using novel sintering technique with sodium bicarbonate as gas generating agent and a polymer eudragit robust formulation was decided on the basis of floating time and in-vitro drug release with possible less polymer concentration, time of exposure and at low temperature. Three batches were prepared with hardness between 4-6 kg/cm2 based on time of exposure at particular sintering temperature desired total floating time, and drug release was retarded up to 12 h. Hence, it is evident from this investigation that a floating matrix tablet using the sintering technique could be promising delivery with improved sustained release action and drug...
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Posted by admin on Mar 31, 2020 in |
A combination of Grazoprevir and Elbasvir is used to treat Hepatitis C virus (HCV). A selective, accurate and precise RP-HPLC method was developed and validated for the simultaneous estimation of these drugs in combined tablet dosage forms. The drugs were resolved on a BDS C18 column using 0.1% orthophosphoric acid: Acetonitrile (45:55 v/v) as the mobile phase. The detection wavelength was 260 nm. The retention times obtained for Grazoprevir and Elbasvir were 2.400 & 3.018 min respectively. The linearity ranges were 25-150 & 12.5-75µg/ml respectively with Regression coefficients of 0.999. The % R.S.D. of precision studies was found to be 0.6 & 0.4 respectively. The accuracy of the proposed method was determined by recovery studies and the mean recovery was 99.14 & 100.34%, respectively. The method was also applicable for quantitative analyses of the marketed tablet formulations and in studying the stability of the drugs under acidic, alkaline, oxidation, thermal and UV...
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Posted by admin on Mar 31, 2020 in |
α-amylase is widely used in various biotechnological applications such as food processing, starch, and paper industries, hydrolyzing starch, glycogen, and other polysaccharides into glucose, maltose, and oligosaccharides. Aspergillus niger α-amylase was partially purified by ammonium sulphate fractionation with 1.409 fold purity and 54.88% yield. The partially purified α-amylase was immobilized on sodium alginate with calcium chloride by ionotropic gelation with glutaraldehyde as a cross-linking agent. Immobilized α-amylase exhibited 50% of its original activity. The soluble and immobilized α-amylases exhibited maximum activity at pH values 7.5 and 8.0, respectively. The optimum temperature for both the soluble and immobilized enzymes was 35 ºC. The immobilized enzyme was more pH and thermally stable than the soluble one. The substrate starch was hydrolyzed by soluble (Km 0.6 mg/ml, Vmax 16.05 mg/ml/min) and immobilized α-amylase (Km 0.65 mg/ml, Vmax 17.41 mg/ml/min) with high efficiencies. On the basis of the results obtained, immobilized α-amylase could be employed in the saccharification of starch...
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Posted by admin on Mar 31, 2020 in |
Globally, the outbreak of antibiotic-resistant pathogens derived infections urged an intensifying necessity for the survey of underexplored marine zone microorganisms for novel metabolites. Our current study emphasized on isolation, polyphasic characterization and bioactivity of the novel actinomycetes from the Bay of Bengal near Kakinada and Nizampatnam, Andhra Pradesh, India, which could provide lead bioactive compounds of therapeutic efficacy to repress the of pervasiveness multidrug-resistant pathogens. A total of fifteen actinobacterial strains (VJRM-1 to VJRM-15) were isolated and tested. Micro-morphological characteristics of the isolates were perceived by SEM (scanning electron microscope) analysis. Phylogenetic analyses of isolates were appraised with 16S rRNA gene sequence homology. The dendrogram was contrived by deploying the parsimony method in MEGA software version 6. Based on polyphasic studies, strains VJRM-7 and VJRM-8 identified as Nocardiopsis dassonvillei (KP299158) and Nocardiopsis sp (KP299159). In-vitro antimicrobial activity of crude ethyl acetate extracts derived from these strains revealed strong antagonistic activity against Gram-positive, Gram-negative bacteria and fungi. Both strains displayed positive results for the production of enzymatic activities. The morpho-physio-molecular...
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