Posted by admin on Feb 29, 2020 in |
Blood parameters are important indicators for assessing the health status of living organisms subject to changing environment. Blood parameters (Hb%, TEC, TLC, ESR, PCV, MCH, and MCV) of Heteropneustes fossilis exposed to lead were investigated at different time intervals. Acclimatized specimens were divided into four groups: Group-A (Control), Group-B (2.25 ppm), Group-C (2.65 ppm), Group-D (2.85 ppm) of Pb and examined at 7, 14, 21 and 28 days’ time intervals. The maximum impact of the treated metal was observed on ESR (Erythrocyte Sedimentation Rate) [1.80 mm/h (Control) to 2.90 mm/h at 2.85 ppm of lead] during maximum time exposure (28 Days) whereas PCV (Packed Cell Volume) showed a fall [44.00 % (control) to 28.00% (2.85 ppm] at 28 Days pe (post-exposure). The other parameters also showed changes in their values when exposed to lead being highest at 2.85 ppm doses during maximum time exposure (28 Days). The above findings indicate that lead adversely affects the blood physiology as reflected in hematological...
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Posted by admin on Feb 29, 2020 in |
Indian literature relating to the spectrum of infections in maintenance hemodialysis patients has not been extensively published. The purpose of this study was to carry out surveillance of infections in this population and to determine the clinical patterns and outcomes of infections and also to assess the prescribed antibiotic therapy. This was a prospective observational study carried out in the dialysis unit of the nephrology department of a tertiary care teaching hospital. The sample of the study comprised of 30 patients who were receiving maintenance hemodialysis and presented with infections. Escherichia coli was the commonly detected gram-negative pathogen in culture and sensitivity tests. Whereas the most commonly isolated gram-positive bacteria were streptococcus pneumonia and methicillin-resistant staphylococcus species which were sensitive to vancomycin. Respiratory system (47.2%) followed by the urinary system (27.3%) were most commonly affected. The most common outcome of infections was a cure (57%). Clarithro-mycin and ofloxacin-ornidazole were found to be inappropriately dose adjusted. Amoxicillin-clavulanate, cefixime, ticarcillin-clavulanic acid, levofloxacin, piperacillin-tazobactam, mero-penem and imipenem-cilastatin were inappropriately adjusted for dosing...
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Posted by admin on Feb 29, 2020 in |
Endophyte is a rich source of bioactive metabolites for medicinal exploitation as an increasing number of novel compounds with bioactive potential are now being isolated from this source. This study was conducted to characterize and explore endophytic fungi from marine weeds available in Bangladesh. The whole weed was used for isolation of endophytic fungi in the water agar media and the isolated fungi were identified through macroscopic and microscopic observation. Small scale cultivation was performed on liquid Wickersham media. Cytotoxicity, antibacterial, antioxidant activities were evaluated following brine shrimp lethality bioassay, disc diffusion method and DPPH free radical scavenging assay, respectively. A total of five endophytic fungi were isolated, purified and characterized from four seaweeds; Hypnea musciformis (HM), Sargassum crassifolium (SC), Dictyota dichotoma (DD) and Caulerpa peltata (CP). They were coded as HMWE-1, HMWE-2, SCWE-1, DDWE-1 and CPWE-1. The fungal strains HMWE-1 and SCWE-1 were identified morphologically as Fusarium sp., HMWE-2 and DDWE-1 as Penicillium sp. and CPWE-1 as Aspergillus sp. DDWE-1 fungal strain showed significant antioxidant activity in comparison...
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Posted by admin on Feb 29, 2020 in |
This study describes the development and validation of stability indicating UPLC method for Bilastine and its impurities, an anti-allergic drug. Bilastine was subjected to stress degradation under different conditions recommended by the International Conference on Harmonization to observe the degradation products. The successful separation of Bilastine from its synthetic impurities and degradation impurities formed under stress conditions were achieved on Acquity UPLC CSH Phenyl-hexyl (2.1 mm × 150 mm, 1.7 µ), and the gradient mode mobile phase consists of 0.05% TFA in water and 0.05% TFA in Acetonitrile. The mobile phase flow rate was 0.10 ml/min. The column temperature was maintained at 25 °C. The sample temperature was maintained at ambient and wavelength fixed at 275 nm UV-detection. It was found that the method of RPUPLC with UV-detection system for the analysis of Bilastine impurities determination and also applied in qualitative and quantitative analysis. The developed UPLC method was validated with respect to specificity, precision, linearity, ruggedness, and robustness. A validation study has been performed as per ICH...
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Posted by admin on Feb 29, 2020 in |
Development of a sustained release liquid oral in-situ gel of Voriconazole with improved bioavailability has been the main aim of this project. Complexation of the drug with hydroxypropyl beta-cyclodextrin was done to improve the solubility and stability of the drug. The mucoadhesive polymer carbopol 934P and release retardant HPMC E50 were used as factors and a two-square factorial design was employed. A simple mixing method was used for the formulation, and evaluation was done. The polymers had a significant effect on gelation time, mucoadhesive strength, and 8 h drug release. Carbopol showed a better- controlled release and mucoadhesive strength than HPMC E50. The experimental values for gelation time, mucoadhesive strength, drug release at 1 h, 8 h, 12 h, and gel strength were found to be 80 sec, 17453 dynes/cm, 21.10%, 62.98%, 90.54%, 55 sec respectively, which were close to the predicted values. Pharmacokinetic studies revealed a 12 h sustained drug release, plasma drug concentrations above 0.5µg/ml (MIC) and 6.76-fold increase in bioavailability. Thermal and photostability studies revealed a...
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