Posted by admin on Dec 31, 2019 in |
Ashwagandhadi lehya is an Ayurveda formulation, official in Ayurvedic Formulary of India and used as aphrodisiac, tonic, rejuvenating agents etc. It is one of the major components of Ashwagandhadi lehya is Withania somnifera which contain Withaferin –A. The study was undertaken to develop an analytical method using HPLC to estimate Withaferin – A, being a marker from, methanolic extract of Ashwagandhadi lehya as one of the quality parameters. Method development involved simple sample preparation without any purification, as well as isocratic separation of Withaferin-A. The method was subjected to validation involving establishing linearity, precision, accuracy, specificity, and robustness.Optimized mobile phase was acetonitrile: water (45: 55 v/v) contained 1% v/v glacial acetic acid. The column used was Phenomenex (250 × 4.6 mm, 5 µm) with flow rate set at 1 ml/min and chromatogram was recorded at 216 nm. The studies showed that peak corresponding to Withaferin – A within concentration range of 1-75 µg/ml was found to be correlated with respective concentration with correlation coefficient (r2) 0.99802 ± 0.0004. The...
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Posted by admin on Dec 31, 2019 in |
The aim of the present study was to formulate an osmotically controlled drug delivery system (O-CDDS) of Metformin hydrochloride to reduce the frequency of multiple dosing in non-insulin dependent diabetes mellitus-II which is a lifelong disease. Metformin hydrochloride a BCS Class-III drug having a poor biological half-life of 6 h. O-CDDS of Metformin hydrochloride is a recent approach for the zero-order release profile. Different factors X1 (effect of percentage of plasticizer), X2 (effect of percentage of release modifier), X3 (effect of percentage of coating) were optimized using central composite design. The granules were prepared by wet granulation technique using PVP K90 as a binding agent. Evaluations of granules like bulk density, tapped density, Hausner’s ratio, carr’s compressibility index, angle of repose was done. Then core tablets were prepared using 16 station tablet press and evaluated for hardness, friability, weight variation. For the preparation of a semi-permeable membrane, the coating of cellulose acetate using polyethylene glycol 400 as a plasticizer was done. Then drilling was done mechanically to create the...
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Posted by admin on Dec 31, 2019 in |
Gmelina arborea Roxb. belongs to the Verbenaceae family, commonly known as Gambhar. It is distributed throughout India, Ceylon, Malayan and Philippine Islands. Its roots have anthelmintic properties. Its flowers are useful in the treatment of leprosy and blood diseases. Its fruit has diuretic properties and aphrodisiac. It is also used in anemia leprosy and vaginal discharges. Leaves have shown analgesic, antimicrobial, and anti-diabetic activities. A new flavone glycosides 5, 7.4ʹ-trihydroxy-8, 3ʹ-dimethoxyflavone- 4ʹ-O-β-D-glucopyranosyl- 7-O-β-D-xylopyranosyl (1→2)-α-L-rhamnopyranoside(A) along with two known compounds Luteolin (B) and Apigenin (C) were isolated from ethanolic extract of the stem parts of the plant. The characterization and structural elucidation of these compounds were carried out by various color reactions, chemical degradation, and spectral analysis. Antioxidant activity of compound A was evaluated by 1, 1-diphenyl-2-pycrylhydrazyl (DPPH) assay system which has shown a significant radical scavenging...
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Posted by admin on Dec 31, 2019 in |
The present study depicts the assessment of class III antiarrhythmic drug dronedarone in its drug substance and drug product. Response surface randomized central composite quadratic design has been employed for the optimization of method parameters using reverse-phase high-performance liquid chromatography (RP-HPLC) on Kromasil C18 250 × 4.6 mm, 5 µ with UV detection at 289 nm. The ranges of the independent variables used for the optimization were flow rate 0.9 to 1.1, column temperature 28 °C to 33 °C and composition of buffer in the mobile phase is 55 to 65%. The influence of these independent variables on the output responses: retention time, asymmetric factor, theoretical plates, plate height and capacity factor were evaluated. The five responses were simultaneous optimized by using central composite design. Optimum conditions chosen for the assay were flow rate of 0.945 ml/min, temperature 31.3 °C and buffer: Acetonitrile has taken in the ratio 61.93: 38.07 respectively. The retention time of dronedarone is 2.356 minutes with the employment of the optimum conditions given by the...
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Posted by admin on Dec 31, 2019 in |
Sarecycline, a narrow spectrum tetracycline-derived antibiotic, is used to treat moderate to severe Acne vulgaris. The current study is aimed at developing a simple, sensitive and accurate liquid chromatography-tandem mass spectrometric (LC/MS/MS) method and validating the same for determination of sarecycline in human plasma. Zorbax, SB C18, 4.6 mm × 75 mm, 3.5 μm, 80 Å column, 10 mM ammonium formate (pH 4.5.): acetonitrile (50:50 v/v) mobile phase was used for chromatographic separation of sarecycline. The sarecycline and minocycline were monitored by electrospray ionization in positive ion multiple reaction monitoring (MRM) modes. This mode was used to detect the sarecycline and minocycline (IS) at m/z 488.6 / 291.1 and 458.4 / 337.2. Liquid-liquid extraction was employed in the extraction of analytes from human plasma. This method is validated over a linear concentration range of 0.5-150.0 ng/mL with a correlation coefficient (r) of ≥ 0.9997. Both drug and internal standards (IS) were stable in plasma...
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