Posted by admin on Dec 31, 2019 in |
A selective, accurate, HPLC method was developed by this study for the determination of rifabutin in bulk and capsule dosage form. This method was developed by SHIMADZU LC-2010 HT using C18 column in solvents methanol: acetonitrile: ammonium acetate buffer (50: 45: 05) as mobile phase. At 1.0 ml/min flow rate the mobile phase was pumped, and the sample was detected at 278 nm. For standard rifabutin the retention time was 4.8 min. The method was validated for analytical standards such as linearity, accuracy, precision, and robustness. In a wide range of 5-25 (µg/ml) the linearity was observed. The method was validated, and a recovery study indicates accuracy of this...
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Posted by admin on Dec 31, 2019 in |
Secondary metabolites not only play a vital role in plant defense but also had shown various medicinal properties which provide a scientific base for using herbs and different plants as alternative medicines that were used by ancient communities. Many of the drugs today are simple synthetic modifications of the natural compounds. The developing commercial requirements of secondary metabolites in recent years has resulted in a great interest in secondary metabolism. Different strategies have been studied with the objective of improving the production of secondary metabolites. Many studies proved the role of plant growth regulators in the accumulation and production of many of these active and therapeutic compounds. Short term experiment was carried out to investigate the response of Catharanthus roseus L. plant to the foliar spray of GA3, IAA and 6-bezylaminopurin at (0, 30, 60, 90 ppm) concentrations during the blooming stage. Three parameters were studied, phenols content, alkaloids content and the antioxidant activity in leaves extracts of this plant. Results showed there were significant increments in all studied...
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Posted by admin on Dec 31, 2019 in |
The treatment of neurodegenerative disorders such as Alzheimer’s disease (AD) becomes much more difficult due to the presence of the blood-brain barrier (BBB). Various synthetic drugs are used for the treatment of Alzheimer’s disease, but the use of herbal products has increased tremendously nowadays. An important medicinal plant widely used therapeutically is Bacopa monnieri (Brahmi), a well-known nootropic herb of family Scrophulariaceae. The bacosides present in Brahmi aid in the repair of damaged neurons and ultimately improve nerve impulse transmission. However, the delivery of every such drug is limited due to the failure of the drug to overcome the blood-brain barrier and reach CNS. Solid lipid nanoparticles (SLNs) are the drug carriers that have been able to penetrate the blood-brain barrier and improve the therapeutic efficacy of drugs. The current study focuses on the treatment strategy for AD using bacoside rich extract loaded SLNs. The bacoside rich concentrate was extracted from the aerial part of the plant and was characterized by melting point determination, HPTLC, UV-spectroscopy and FTIR. SLNs...
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Posted by admin on Dec 31, 2019 in |
Atenolol is a hypertension drug that has a low solubility characteristic in water and gastric fluid. The rate of absorption of the drug with poor solubility characteristics is determined by the dissolution process. In this study, an attempt has been conducted to increase the dissolution of atenolol by increasing its solubility. The solubility of atenolol has been enhanced by the inclusion complex using β-cyclodextrin made by several methods (physical mixing, kneading, and solvent evaporation). Evaluation and characterization of atenolol-β-cyclodextrin inclusion complex consist of drug content, dissolution test, Fourier Transformed Infrared analysis (FT-IR), Differential Scanning Calorimetry (DSC), X-ray Diffraction (XRD) and Scanning Electron Microscope (SEM). The results of the drug content analysis, dissolution test, and characterization showed that atenolol- β-cyclodextrin inclusion complex, which has been made by the solvent evaporation method was the best approach. Therefore, a solvent evaporation method was chosen to formulate orally disintegrating tablets of atenolol-β-cyclodextrin using direct compression technique. Orally disintegrating tablets of atenolol-β-cyclodextrin were prepared using crospovidone as disintegrant. The results of pre-compression test and...
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Posted by admin on Dec 31, 2019 in |
Ayurveda is the holistic approach towards life, health, disease management through medicinal herbs minerals, diet and lifestyle leads to the great need for standardization of herbal medicine to maintain its safety and efficacy. Mukta bhasma is used in the treatment of bone metabolic disorders associated with calcium deficiency. It was prepared by Shodhana, Marana and Sharava samputa and the Standardization of bhasma are very necessary to confirm its identity and to determine its quality and purity. An attempt has been made to summarize the ancient and the advanced methods available for the standardization of bhasma. The dosage uniformity and patient compliance can be increased and adulteration can be decreased in ayurvedic powders by formulating them into tablets. The aim of the present work is to develop and evaluate Mukta Bhasma tablets using starch and acacia as a binder. The granules were prepared by the wet granulation method. The prepared tablets were evaluated for different parameters, acute toxicity of Mukta bhasma was conducted on albino rats. Mukta bhasma was administered...
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