Posted by admin on Mar 31, 2020 in |
Objective: Mycoplasma hominisis a gram-negative bacteria belonging to class Mollicutes that is commonly present in men and women of reproductive age. At present, there is no effective prophylaxis for Mycoplasma hominis, as it has gained resistance for the drugs hence an in-silico approach was undertaken to find out the peptide-based vaccine for the pathogen. Methods: In women, it affects the genital tract and involved in causing pelvic inflammatory disease, ectopic pregnancy, early delivery, miscarriage and prolonged infection may lead to infertility. M. hominis can also be transmitted from mother during childbirth and cause fever and infection in the new-born baby. All the 529 protein sequences of Mycoplasma hominis were taken in FASTA format from the UniProt proteome database. Antigenicity study was done for all the proteins of the pathogen using VaxiJen v2.0 and the proteins with high antigenicity scores were taken for further analysis like structural and functional analysis using InterPro, molecular docking using Autodock and molecular simulation study was carried out using Charmm. Results: The study identifies 50S ribosomal protein L28,...
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Posted by admin on Mar 31, 2020 in |
Lymecycline is a tetracycline broad-spectrum antibiotic. Present work describes a simple, accurate, precise, economical and reproducible spectrophotometric method in ultraviolet region has been developed and validated for the assay of Lymecycline in bulk and in Pharmaceutical formulations in diluent. Lymecycline is a tetracycline broad-spectrum antibiotic. Lymecycline like other tetracyclines is used to treat a range of infections Lymecycline exhibits absorption maxima at 270 nm in diluent. Beer’s law was found to be obeyed in the concentration range of 7.5-22.5 μg/ml. The optimum concentration of the Lymecycline was found to be 15 µg/ml. This concentration of Lymecycline was shown good absorbance valves at respective wavelengths was found to be 0.490. Linearity studies were carried out and the range was found to be 7.5-22.5 µg/ml for Lymecycline in diluent. The regression coefficient value of Lymecycline was found to be 0.999 which was not less than 0.995. The method is accurate, precise and economical. In this proposed method, there was no interference from common pharmaceutical excipients. The results of the analysis were...
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Posted by admin on Mar 31, 2020 in |
A number of strategies to deliver antifungal using nanocarriers are developed to facilitate drug targeting infected cells that result in improved antifungal activity. Objective: Ciclopirox olamine has a broad spectrum of action against dermatophytes, yeasts, filamentous fungi and bacteria. It has a biological half-life of 1.7 h and bioavailability of < 5% with prolonged use. A remarkable feature of the drug is that no single case of fungal resistance has been reported so far. Therefore, ciclopirox olamine loaded niosomes entrapped in suitable gel delivery systems may successfully deliver the drug for prolonged time in treatment of fungal infection. Material and Method: A Ciclopirox Olamine loaded niosomes were developed using various surfactants along with cholesterol in different proportions by ether injection and film hydration method. The prepared niosomes were evaluated for various characteristics such as shape and size, entrapment efficiency, in-vitro drug release studies. Result and Discussion: Among all, formulation CNFS61 showed optimal drug release with maximum entrapment efficiency having smaller vesicular size. So, formulation CNFS61 was considered as promising...
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Posted by admin on Mar 31, 2020 in |
Possibility of use of the informational parameters, which characterize adaptational and regenerative opportunities of organ, as potential criteria for the assessment of biological activity of tissue preparations, is investigated in this research. The influence of tissue preparation «STEMB» on the morphofunctional condition of a liver of rats at experimental toxic damage of organs was studied. The assessment of a morphofunctional condition of a liver under the influence of preparation was carried out as with use of traditional morphological, biochemical and histologic parameters, and also by means of informational parameters. A correlation analysis was conducted between the used biochemical, morphological and morphometric parameters and informational criteria. As a result of research it is established that hepatoprotective activity of preparation «STEMB» is confirmed both with results of traditional techniques of an assessment and with informational parameters, that allows drawing a conclusion on possibility of use of informational parameters as criterion of an estimation of biological activity of tissue...
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Posted by admin on Mar 31, 2020 in |
Munronia pinnata is a rare medicinal plant, naturally distributed in China, India, Vietnam, Indonesia, Malaysia, Philippines, and Sri Lanka. The entire plant is extensively used for medicinal purposes, especially for curing skin diseases and treating fever, including malaria. Four major ecotypes were identified in Sri Lanka that consist of 3, 5, 7, and 9 leaflets from various regions. This study was performed to investigate the phytochemical activity of four different ecotypes of Munronia. Leaves of ecotypes were collected and dried at 45 ºC for 15 h. The methanol extracts were prepared and used for screening of antioxidant activity, total polyphenol content, antifungal, and anti-diabetic activity. The findings were indicated that all ecotypes attest to significant differences in its activity but to varying degrees. The strongest antioxidant activity, highest polyphenol content, and mild inhibitory activity for Cladosporium cladosporioides were observed in ecotype with 3 leaflets. Ecotype with 7 and 9 leaflets showed moderate activity, and ecotype with 5 leaflets was the lowest for all tested properties. None of the ecotypes...
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