Posted by admin on Nov 30, 2019 in |
A reverse-phase high-performance liquid chromate-graphic method (RP-HPLC) was developed for the simultaneous estimation of combined dosage form of ezetimibe with simvastatin and rosuvastatin using RP-C18 column. For estimation of ezetimibe and simvastatin, the mobile phase (acetonitrile: water and pH adjusted to 4.5 with acetic acid) was pumped at a flow rate of 0.8 ml/min in the ratio of 85:15% v/v and the eluents were monitored at 234 nm. For the estimation of ezetimibe and rosuvastatin the mobile phase (acetonitrile: water and pH adjusted to 4.5 with acetic acid) was pumped at a flow rate of 0.8 ml/min in the ratio of 75:25% v/v and the eluents ezetimibe and rosuvastatin were monitored at 245 nm. Linearity was obtained in the concentration range of 2-12 μg/ml for ezetimibe in both the mobile phase, 2-12 μg/ml for simvastatin, and 2-12 μg/ml for rosuvastatin. The method was statistically validated, and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed method. Due to its simplicity,...
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Posted by admin on Nov 30, 2019 in |
A simple, sensitive and stability indicating UV spectrophotometric method was developed and validated for the estimation of caffeine in a gel formulation, plasma and urine samples using acidified alcohol as solvent. At absorbance maxima of 265 nm, the linearity was found to be in range of 4-20 µg/ml with correlation coefficient 0.9988. The method was applied for estimation of caffeine in a gel formulation, plasma, and urine samples and the results show excellent recoveries. The method was validated statistically and by recovery studies for linearity, precision, repeatability, and reproducibility. The caffeine was exposed to thermal, photolytic, hydrolytic, and oxidative stress conditions, and the stressed samples were analyzed using the proposed method to demonstrate the specificity of the method. The stated method can be used as a stability-indicating method with high degree of linearity, accuracy and precision for estimation of caffeine in gel formulation and biological...
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Posted by admin on Nov 30, 2019 in |
The aim of present work is to design a Tenoxicam loaded nanogel for the anti-inflammatory and analgesic effect of prepared nanogel. Tenoxicam is one of the NSAIDs of oxicam class; it is very potent and useful for chronic use in rheumatoid arthritis and osteriostatis. The formulations of nanogel based materials have high drug loading capacity, biocompatibility, and biodegradability which are the key points to design a drug delivery system effectively. This research article is to succinctly describe the recent development of nanogel drug delivery system concerning drug loading and swelling of drug from nanogel. To change the mode of administration of Tenoxicam for increasing its pharmacological action we prepared Tenoxicam loaded nanogel which is prepared by modified emulsification-diffusion method by using swelled polymer like noveon polycarbophil AA-1. The formulated nanogel was evaluated for particle size, zeta potential, drug content, entrapment efficiency, in-vitro drug diffusion studies, in-vitro permeation...
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Posted by admin on Nov 30, 2019 in |
The present exploration is an attempt to an ethnobotanical study was conducted in Eastern Ghats of Tamil Nadu, for the investigation of a medicinal plant used to treat several diseases by the local health healers by Gedamalai Tribes, Namakkal District. Data were collected during March 2015-January 2016. Quantitative analysis, including the informant consensus factor and use-value was performed to evaluate the medicinal plants. A total of 40% of women reported high affiliation with herbal remedies for gynecological problems, while others attribute was also positive for medicinal plants. A total of 27 species belonging to 23 genera and 19 families were recorded. This result also proved that the plant is used either the whole plant or various parts like leaves, stem, root, flower, seed, etc. The plant parts are used in the form of decoction, juice, paste, and powder. These plants were used to treat gynecological disorders. The botanical names, families, vernacular names were also given along with medicinal...
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Posted by admin on Nov 30, 2019 in |
Alzheimer’s disease (AD) is a disorder of the central nervous system with progressive neurodegeneration, cognition, and memory loss. A major molecular hallmark of the disorder includes extracellular deposition of the Amyloid beta-peptide (Aβ) in senile plaques, the appearance of intracellular neurofibrillary tangles. The Aβ peptide is produced by sequential cleavage of the Amyloid precursor protein (APP) by α, β, and γ-secretase. The secretase β and γ generate a number of isoforms of peptides containing 36-43 amino acid residues in length. The mainly universal isoforms are Aβ(1-40) and Aβ(1-42). Aβ(1-42) C-terminal domain (29-42 amino acid residues) adopts β conformation, and the N-terminal domain (10-24 amino acid residues) facilitates a dynamic equilibrium between α- helix and β-strand. Thus Aβ polymerization requires unfolding of the native α-helical structure of Aβ. Stabilization of the Aβ central α- helix is an efficient step to foil the Aβ polymerization. Here we report test compounds which to bind and stabilize the 11-30 amino acid regions of Aβ(1-42) in α-helical conformation. On docking, the ligands with Aβ...
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