Posted by admin on Jan 31, 2020 in |
Objective: Formulation and development of polymeric microparticles containing murrayanol for better treatment of diabetes mellitus. Methods: Successive solvent extraction of leaves of Murraya koenigii plant was done. The active constituent of leaves that is murrayanol was isolated and identified through HPLC and FTIR. Polymeric microparticles were selected as a carrier system and formulated by the solvent evaporation method. Characterization and performance evaluation of particulate system loaded with herbal plant extract of the Murraya koenigii leaves was done. Results: These results suggest that the polymeric microparticles are not only effective in the rapid attainment of high drug concentration in the body and also maintain the same over a prolonged period of time. Microparticles were formulated and characterized for the particle size, shape and its distribution, percentage drug entrapment and in-vitro drug release profile along with the stability studies. In-vivo tissue distribution studies on albino rats suggested the accumulation of formulations in the different organs. Conclusion: Prolonged release of natural drug from the carrier system, decrease the dosing frequency and also...
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Posted by admin on Jan 31, 2020 in |
Biofilms are communities of bacteria that are append to a surface and play significant role in the persistence diligence of bacterial infections. Bacteria with a biofilm are more defiant to antibiotics compared with planktonic bacteria. Biofilm producing bacteria are able to take possession on medical devices such as catheters and implants. The incidence of Biofilm producing microorganism runs around 80% of infections. These kinds of infections are difficult to make a diagnosis and treat. The present investigation has been done to analyze the correlation of MDR Pseudomonas sp with production of Biofilm, MBL production and Serum resistant activity. 63 (12.6%) Pseudomonas isolates were incurred from a total of 500 clinical samples and were speciated based on both phenotypic and genotypic methods. Among the total isolates detected 56.2% were positive for both Biofilm production and Serum resistant activity. MBL productions were observed in 70.83% of the isolates. The significance of the result also relates that all the isolates expressed high MAR index values that confirm the association between the virulence...
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Posted by admin on Jan 31, 2020 in |
During the past few years many phytochemicals responsible for anti-diabetic effects have been isolated from the plants. Several phyto-constituents such as alkaloids, glycosides, flavonoids, saponins, dietary fibers, polysaccharides, glycolipids, peptidoglycans, amino acids and others obtained from various plant sources that have been reported as potent hypoglycemic agents. In the present study, the effect of methanolic extract of whole plant of Lindernia ciliata (LCME) (100, 200 and 400 mg/kg) on glucose levels in streptozotocin (STZ) (45 mg/kg) induced diabetic rats was investigated using glibenclamide 10 mg/kg as the positive control. The effect of the extract on body weight, insulin, total cholesterol, triglycerides, serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP), total protein, serum urea, creatinine and hematological indices in normal, treated and untreated diabetic rats was investigated. Induction of diabetes in male Wistar rats (200-250 g) resulted in increased levels of serum glucose, total cholesterol, triglycerides, SGOT, SGPT, ALP, urea and creatinine and decreased bodyweight, serum insulin, total protein, RBC, WBC and hemoglobin levels. Administration...
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Posted by admin on Jan 31, 2020 in |
Changes in the hemolymph protein and trehalose contents of the silkworm, Bombyx mori L. due to Streptococcus mutans infection and antibiotics treatment was evaluated. Interestingly, S. mutans, which was successfully grown in the body of the larvae led to decline 69.60 and 50.21% of protein content in the hemolymph. Concomitantly, there was a drastic rise in the content of trehalose to 60.22 and 65.13% due to bacterial infection-induced through hemocoel and oral routes respectively compared to healthy larval batches. Antibiotics clinically advocated to cure dental caries was substantially lowered the bacterial infection in the silkworm larvae regulating a rise in the protein and reduction in the trehalose contents. Notably, antibiotic-Advent treatment through hemocoel and oral routes explicit higher rate of recovery in the synthesis of protein that amounts to 133.79 and 92.62%, while trehalose content was found 34.30 and 40.31% less in the hemolymph that equivalent to healthy larvae respectively. Similarly, taxim was also influenced for an elevation of protein content 106.73 and 78.99%, whereas there was a reduction...
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Posted by admin on Jan 31, 2020 in |
Hepatotoxicity in the form of acute liver failure is a serious side effect of several drugs most importantly paracetamol. Beetroot main ingredients are nitrate, betalains, phenolics and ascorbic acid, which are responsible for its protective against oxidative stress and inflammation. Aim: This study assessed the possible protective effect of beetroot against liver toxicity induced by paracetamol. Method: Forty adult male rats where divided into four groups (ten/each): control groups, group I and II (with and without beetroot juice), group III, paracetamol treated group (400 mg/kg/day), and group IV protected group (paracetamol concomitant with beetroot juice by gavage 2 ml/day) for one month. Blood samples and liver tissues were collected and subjected to biochemical and microscopic analysis, respectively. Results: Results demonstrated that paracetamol consumption, group III, induced toxicity to the liver evident by high ALT and AST. The intake of beetroot juice concomitantly with paracetamol (group IV) ameliorates its side effects supported by histological findings and liver function chemistry results. Group IV showed a decreased ALT and AST levels to...
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