Posted by admin on Nov 30, 2019 in |
In recent years, there is an increasing interest in finding antioxidant phytochemicals for the prevention and treatment of complex diseases like diabetes, cancer, and atherosclerosis, which are associated with excessive biological oxidative stress. Plants are rich source of natural antioxidants that inhibit the propagation of free radical reactions and protect the human body from degenerative diseases. For the present study, entire vine (aerial part) of Cyclea peltata was selected based on their extensive use in folk medicine and ayurvedic medicine to cure various ailments. Hexane, chloroform, ethyl acetate, methanol and water extracts of Cyclea peltata were subjected to various in-vitro antioxidant activity studies. In most of the antioxidant parameters, ethyl acetate and methanol extracts showed potent activity and chloroform extract showed minimum antioxidant activity. From these studies, it can be concluded that ethyl acetate and methanol extracts of Cyclea peltata could be used as a source of potent natural antioxidant that can be used in various plant-based drugs or formulations for the treatment of complex diseases caused due to...
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Posted by admin on Nov 30, 2019 in |
In different myeloid leukemia cases resistance to diverse treatment regimen owes to multiple molecular mechanisms of cellular network. Epigenetic dysregulation, gene mutations, overexpression of multidrug resistance genes, abnormal immune function, the presence of chemotherapy-resistant leukemia-initiating cells, and aberrant signaling pathways could be the lead cause of this kind of aberration. Some marketed drug is considered as a first-generation drug that can inhibit the enzymatic action by inhibiting the ATP binding with different key pathway regulator protein. Later on, insensitivity of myeloid leukemia cells towards these drugs has been observed may be due to mutation in tyrosine kinase domain of the kinase receptor. By enchanting into account of bioavailability and resistance developed, there is an extreme need to find some natural and nature-derived inhibitors for the kinase proteins of different key metabolic pathways. For computational screening and in-vitro studies against cancer cell lines and marker proteins, Mangiferin and Euxanthic acid isolated from leaves of Mangifera indiaca had been used as mentioned in Ayurveda / Indian Traditional Medicine (ITM). Docking analysis...
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Posted by admin on Nov 30, 2019 in |
Objective: Wedelolactone is a naturally (WDL) occurring coumestan of phytoestrogen category that possess anticancer and anti-inflammatory property. In this study, the anti-inflammatory effect of WDL on human cervical cancer cells (HeLa) and initiation of apoptosis mechanism were investigated. Methods: MTT assay and Trypan blue assay were demonstrated to detect the inhibitory effects of WDL on cell proliferation. The mRNA expression of IL-1β, TNFα, IL-6, TGFβ, and NFκB were detected by RT PCR. Western blotting was performed to detect the expression of apoptosis-associated proteins; cleaved caspase-3, cleaved PARP, Bcl-2, and Bax. Results: WDL significantly inhibited the growth and proliferation of HeLa cells in a concentration dependent manner with the IC50 of 10µM. mRNA expression of inflammatory cytokines and NFκB was significantly reduced in WDL treated groups. Moreover, WDL promoted the apoptosis of HeLa cells dose-dependently by down-regulating Bcl-2 and up-regulating cleaved caspase-3, cleaved PARP, and Bax. Conclusion: Collectively, WDL exerted anti-inflammatory effect through the suppression of NF-kB activation, IL-1β, TNFα, IL-6 and promoted apoptosis in HeLa cells. Thus, use of...
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Posted by admin on Nov 30, 2019 in |
The medicinal plants, Boerhavia diffusa and Cephalandra indica are both perennial climbing herbs commonly used in traditional system of medicine for the treatment of several ailments because of their healing properties. The present study aimed to identify the phytochemical constituents of methanolic extracts of these plants, followed by their metabolic fingerprinting, which was carried out by HPTLC and GC-MS analysis. Total phenol and flavonoid content were measured, followed by a radical scavenging assessment using 1,1, Diphenyl-2-picrylhydrazyl (DPPH) radical system. This preliminary data showed encouraging results for the presence of a significant amount of flavonoid, phenols, and anti-oxidant activities. The HPTLC chromatogram of both methanolic extracts showed a variety of metabolites having different Rf value suggesting the diverse nature of phytoconstituents. Further, the GC-MS analysis revealed the manifestation of at least 23 and 22 metabolites of B. diffusa and C. indica, respectively. These metabolites were identified as fatty acids, sugar alcohols, large carbohydrates, phenols, and flavonoids possessing potential antioxidant, antitumor, anti-hyperglycemic, and antimicrobial properties. The results of the present study...
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Posted by admin on Nov 30, 2019 in |
In the present research, newly developed hydrotropic solid dispersion technique utilizing the use of aqueous solvent was developed, to avoid the utilization of organic solvent and all together declining their toxicity. This technology was employ intended for preparing the dispersion of Eluxadoline by using new hydrotropic agent sodium caprylate with using different ratio. Prepared formulations were evaluated by Solubility study, Fourier transforms infrared spectroscopy, X-Ray Diffractometry, Drug content analysis, and in-vitro drug release study. Based on the evaluation of hydrotropic solid dispersion concluded that the concept or technology of hydrotropic solid dispersion method is a novel, nontoxic and as well cost-effective technique for the rationale of enhancing the aqueous solubility of substance and bioavailability of poorly water-soluble drugs. The improvement within solubility and dissolution of Eluxadoline is a clear sign. It can be able to use in the future for more poorly water-soluble drugs to enhance solubility and also which having low bioavailability is a major...
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