Posted by admin on Nov 30, 2019 in |
Objective: The present study was designed for Isolation of phytoconstituents from pharmacologically potent extracts of leaves of Cassia uniflora based on in-vitro pharmacological screening and their subsequent characterization. Methods: Crude extracts of leaves, stems, and fruits of cassia uniflora were prepared using various solvents such as water, methanol and hydro alcohol. These extracts were screened for in-vitro pharmacological activities like antioxidant, anti-inflammatory, and anti-diabetic activities. These active extracts were subjected to column chromatography by means of different mobile phases followed by TLC. The isolated compounds were subjected to IR, 1H NMR, 13C NMR, and LC-MS spectral analysis for chemical characterization. Results: The methanol extract of leaf of Cassia uniflora was found to be potent when compared with other extracts. This extract was subjected to column chromatography to get fractions and eluted fractions were run in TLC mobile phase with different solvent ratios. The fractions with similar Rf values to standard were united and crystallized. The spectral analysis confirmed that the isolated compounds were found to be Methyl inositol, Luteolin,...
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Posted by admin on Nov 30, 2019 in |
Delonix elata (D. elata) whole plant was chosen to evaluate in-vitro anti-oxidant activities. The total polyphenols and flavonoids were determined after the extraction. DPPH (2, 2′-diphenyl-1-picrylhydrazy), H2O2 (Hydrogen peroxide), NO (Nitric oxide) and TBARS (Thiobarbituric acid) methods were used to evaluate antioxidant activities of D. elata whole plant. The results showed that the highest levels of polyphenols were recorded in DEM (15.32 ± 0.32 mg of GA/g of extract). Total flavonoid contents of extracts/fractions showed that DEM (15.57 ± 0.69 mg of Qu/g of extract) contains the highest level. The DEM extract shows more inhibition of DPPH (92.56), NO (143.88), H2O2 (182.28) and TBARS (121.94 µg/ml), respectively when compared with other extracts. These findings suggest that the extracts obtained from D. elata stem have active substances contributing to the increase in natural antioxidant potential. It can be concluded from the findings that D. elata extracts have potent antioxidant...
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Posted by admin on Nov 30, 2019 in |
Objective: To explore the Teratogenicity of isolated Polyphenols of Symphyocladia latiuscula (PSL) on pregnant female rats. Experimental Design: The marine alga Symphyocladia latiuscula were the preparation of methanolic extract followed by isolation of Polyphenols. The Teratogenic study was performed according to the OECD guidelines, no 415. And the test drug; PSL 100, 200 and 1000 mg/kg and positive control Valproic acid (VPA); 1000 mg/kg was administered from 6th to 17th day of gestation during the period of fetal organogenesis. All females were killed on the 20th day of pregnancy, and their uterus was dissected to examine the Gross Abnormalities. Results and Conclusion: VPA significantly reduced all body measurements in terms of growth retardation, body weight, height, lower and upper limb, VPA showed mortality of 2 pups in the concentration of 1000 mg/kg/bw i.v whereas in PSL no significant differences were noted concerning the positive control group. The highest difference was shown especially PSL 1000 mg/kg/bw fetal morphological abnormalities; regarding the weight and length of the fetuses, there was a...
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Posted by admin on Oct 31, 2019 in |
A simple and reliable head space Gas Chromatographic method has been developed for the determination of residual ethanol, isopropyl alcohol and toluene in Amiodarone hydrochloride drug substance. The proposed method is based on flame ionization detection technique with DB 624 as stationary phase. Linearity of detector response was established up to 150 % of the limit level for ethanol, isopropyl alcohol and toluene and the quantitation limit was 80 ppm for ethanol, 40 ppm for isopropyl alcohol and 4 ppm for toluene respectively. Performance of the method was assessed by evaluating the recovery, repeatability, reproducibility, linearity and limits of detection and quantification. The proposed method has a potential for application to drug substances which may contains traces of residual solvents. Results prove that the validated method was suitable for determining the residual ethanol, isopropyl alcohol and toluene in Amiodarone hydrochloride drug...
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Posted by admin on Oct 31, 2019 in |
Four isatin mixed ligand complexes of the type ML1L2, where M =Co(II), Ni(II), Cu(II) and Zn(II) (LI = Schiff base formed by the condensation of isatin and p-nitroaniline and L2= Schiff base formed by the condensation of isatin and 2,4-dinitroaniline) are synthesized. Mixed ligand complexes are different from routine metal complexes that they have a minimum of two special kinds of ligands linked to the one metal ion in a complex. Ethanol is used as a solvent for the synthesis of ligands and their mixed ligand complexes. All the mixed ligand complexes have been characterized by various physicochemical techniques such as Elemental analysis, IR, UV-Visible, conductivity measurements, magnetic moment measurements, thermogravimetric analysis, etc. Tetrahedral geometry is assigned for all the mixed ligand complexes. Here both the ligands are bidentate and potent donors are carbonyl oxygen and azomethine nitrogen atoms. The ligands and the mixed ligand complexes are screened for cytotoxicity and antioxidant properties. The mixed ligand metal complexes show higher antioxidant and cytotoxic activities as compared to the...
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