Posted by admin on Nov 30, 2019 in |
The research work focused on the optimization of Terbinafine HCl (TBH) pluronic lecithin organogels (PLOs) for increasing the penetration and efficacy of the drug. The factors affecting the PLOs such as viscosity and percentage drug release, were screened and optimized using the Box-Behnken design. The optimum formulation based on desirability (0.995) exhibited 3139.92 Cp for viscosity and 93.4561% for drug release. The optimized PLO was evaluated for drug content, pH, in-vitro drug release and ex-vivo studies. The percentage cumulative release of TBH permeated from optimized PLOs and marketed cream (MC) at the end of 12 h after the application was found to be 60.29 ± 0.69% and 19.35 ± 2.19% respectively. The comparatively higher permeability TBH in optimized PLOs could be due to increased solubility and diffusion coefficient of the drug. In-vitro microbial assay, studies showed that the PLO reduced the fungal burden of Candida albicans as compared to M.C (0.34 ± 0.08 mm) in a shorter duration of time with a larger zone of inhibition (0.58 ± 0.12...
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Posted by admin on Nov 30, 2019 in |
This study aimed to investigate the preparation and characterization of polymeric nanoparticles containing extract of Andrographis paniculata using Eudragit S 100 polymer. The methanolic extract of aerial parts of Andrographis paniculata Nees (Acanthaceae) was prepared and standardized for andrographolide content using the High-Performance Liquid chromatography technique. Three formulations containing 50, 100 and 150 mg of extract were prepared by solvent displacement method. Parameters including drug: polymer ratio, stabilizer, solvent, and stirring speed were optimized. Characterization of the formulations was carried out using particle size analysis, PDI, DSC, X-RD, SEM techniques, entrapment efficiency and in-vitro drug release studies. The particle size of the nanoformulations was found to be within the range of 300-400 nm, with entrapment efficiency more than 75% and drug release more than 55% after 8 h. Aerial parts extract of Andrographis paniculata was embedded and dispersed in Eudragit S 100 polymer as smooth spherical nanoparticles which may prove to be a novel drug delivery...
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Posted by admin on Nov 30, 2019 in |
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for quantification of Darunavir in bulk and its tablet dosage form. The separation was carried out on Zorbax Eclipse XDB C18 (150 × 4.6 mm; 5 µm) column at 35 °C temperature using ammonium acetate buffer as mobile phase-A, acetonitrile (100%) as mobile phase-B. The flow rate was 1.0 ml/min and effluent was detected at 265 nm. The retention time of Darunavir propylene glycolate was 31.34 min. The percentage recovery was within the range between 95.46% and 100.17% for Darunavir propylene glycolate. The linear ranges were found to be 0.086-3.084 µg/ml (r2 = 0.9997) for Darunavir propylene glycolate. The percentage relative standard deviation for accuracy and precision was found to be less than 5.0%. Hence, the method could be successfully applied for routine analysis of Darunavir in pharmaceutical...
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Posted by admin on Nov 30, 2019 in |
The blood-brain barrier is the most challenging physiological obstacle for drug moieties for reaching the central nervous system. Meropenem is a drug used against meningitis, however crossing BBB is also a challenging realm of problem for the same. Therefore attempt has been undertaken to strategically design Meropenem loaded cholesterol-soya lecithin nanoliposomes (MCSLN). MCSLN was prepared by nano-emulsification technique with tween 80 as its surface ligand. Molecular docking studies revealed that tween 80 interacts with apolipoprotein E through π-π interactions and hydrogen bonding, which might be its mechanism for transmembrane permeation across BBB. After characterization, in-vitro release study was carried out to evaluate cross-walking efficiency of liposomal drug delivery system through the simulated blood-brain barrier. Entrapment efficiency estimation showed that about 80% of the drug has been successfully entrapped within the liposome. Dynamic light scattering study exhibited bimodal distribution of particle with size ranges 70-75 nm and 300-350 nm. Zeta potential reveals good stability of the formulation. FTIR studies reveal that Meropenem has been successfully entrapped within the formulation. On...
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Posted by admin on Nov 30, 2019 in |
As a part of our ongoing effect to develop a biodegradable medicated film for wound healing using chitosan and HPMC as polymers. The drug used is Silver sulfadiazine. This formulation produces better biodegradability and bioadhesion. The biodegradable film is non-toxic and non-irritant to the skin. An animal wound model was performed on the back of the rats and treated, respectively with medicated biodegradable film and with a marketed product. There are no infections found during the period of study. The wound healed within 6 days, in the case of medicated film formulation, but not with the marketed cream. Medicated films could be a good alternative to conventional wound dressings, which is painful and injurious. The film adheres to open wounds, and therefore it is good protection providing a moist healing environment. Better patient compliance is an added advantage as it does not require repeated...
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