Posted by admin on Feb 29, 2020 in |
Rheumatoid arthritis is an autoimmune disease that involves inflammation of joints. Diclofenac diethylamine, as well as Curcumin, are widely used for symptomatic relief of pain and inflammation. The present study was aimed at providing a simple, accurate and economical spectrophotometric method for simultaneous determination of curcumin and diclofenac diethylamine from topical gel formulation. Simultaneous determination of both drugs namely, curcumin and diclofenac diethylamine was done by Absorbance correction method using phosphate buffer 5.5: ethanol (1:1) as the solvent. A topical gel containing curcumin microspheres and diethylamine were prepared using carbopo l971 as a gelling agent along with propylene glycol, preservatives, and triethanolamine. Diclofenac diethylamine showed an absorbance maximum at 278 nm whereas curcumin showed at 428.4 nm in phosphate buffer 5. 5: ethanol (1:1). The linearity of both drugs was found in the concentration range 2-26 μg/ml for Diclofenac diethylamine and 1-7 μg/ml for Curcumin respectively. This method was then applied successfully to pharmaceutical dosage form gel and no interference of excipients was found. Recovery for curcumin and diclofenac...
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Posted by admin on Feb 29, 2020 in |
Chemically, rhein is 9, 10-Dihydro-4, 5-dihydroxy-9, 10-dioxo-2-anthracenecarboxylic acid, and use as an intermediate to synthesize diacerein which widely used in the treatment of degenerative diseases like, osteoarthritis, osteoporosis, and rheumatoid arthritis and hence has commercial importance. Rhein occurs in free state in nature in different plant families like Cassia, Rhamnus, and Rheum but in very less concentration and as it is currently subject of interest because of its anti-tumor, anti-viral and anti-oxidant properties, therefore, there is need for simple and efficient isolation and extraction method to obtain rhein from available indigenous plant sources. A modified method developed for the isolation of rhein from Cassia angustifolia leaves is described, the method involves oxidative hydrolysis of sennoside with help of an oxidizing agent followed by the isolation of the rhein from reaction mixture, and with substantial yield improvement is achieved. The objective of this study to optimize yield of the rhein from senna semisynthetically. The isolated rhein was analyzed by spectral analysis for its...
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Posted by admin on Feb 29, 2020 in |
The objective of the present study is to develop, evaluate and compare potent phytoconstituents of curcumin loaded solid lipid nanoparticles (CUR-SLN) and Paclitaxel loaded solid lipid nanoparticles (PTX-SLN) for the treatment of malignancy. Curcumin is an active phenolic compound of Curcuma longa as a promising phytoconstituent has been extensively studied over the past several years. The result of these studies has indicated that the curcumin (CUR) acts on multiple molecular targets to selectively kill tumor cells with low intrinsic toxicity. The well-known anticancer agent Paclitaxel (PTX) is used for its effectiveness in the treatment of a wide range of tumors cells. However, due to the hydrophobic nature of PTX limits its effective application as anticancer drug. This study utilizes the immense potential of nanotechnology, particularly SLNs, in improving safety & bioavailability of CUR and PTX. SLN was chosen due to their excellent lipoidal drug carrier properties, physiological compatibility, and economic standpoint. In this study physical mixture of CUR-SLN and PTX-SLN formulation and PTX-SLN formulation were examined on Ehrlich ascites...
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Posted by admin on Feb 29, 2020 in |
Rice (Oryza sativa L.) is one of the most important cereal crops consumed in Asia and the primary staple food being consumed by nearly half of the world’s population. Black rice is getting popular recently and is consumed as a functional food. Black rice contains relatively high anthocyanin in the pericarp layer which gives the dark purple color. Black rice has a higher content of phenolic compounds as compared to white rice. The total phenolic content was four times higher in pigmented rice than in non-pigmented varieties. Heat Moisture Treatment (HMT) and enzymatic modification are essential processes prior to absorption in the human body as they affect digestibility. On the basis of these considerations, the dual modification applied in this study was a combination of heat-moisture treatment (HMT) and enzymatic modification. The samples used for the study were native and dual modified rice flour of selected white and black rice variety. The samples were termed as Native White Rice Flour (NWRF), Dual Modified White Rice Flour (DMWRF), Native Black...
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Posted by admin on Feb 29, 2020 in |
Arsenic and mercury are presently the most common pollutants of freshwater bodies. There is a continuous increase in the toxic level of these pollutants with some seasonal changes, affecting the aquatic biota. The present study aimed to identify cellular biomarkers of arsenic and mercury toxicity in freshwater fish Channa punctatus. Antioxidant defense like catalase (CAT), superoxide dismutase (SOD), ascorbate, reduced glutathione (GSH), oxidative stress marker lipid peroxidation (LPO), lysosomal marker like acid phosphatases and apoptotic marker namely caspases-3 were employed to check the damage caused to the fish as a result of arsenic and mercury contamination of water. Results indicate that increased lipid peroxidation induced apoptosis in arsenic toxicity. On the other hand, mercury toxicity induced necrosis mediated by lysosomal induction. These results further significantly indicate the protective effects of ascorbic acid that reduces the increased level of hepatic oxidative stress during metal toxicity. This study suggests that ascorbic acid supplementation can be a good option to save fish, which are at high risk of heavy metal-induced damage resulting...
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