Posted by admin on Oct 31, 2019 in |
Indian medicinal plants are considered as a vast source of several pharmacologically active principles and compounds, which are commonly used in home remedies against multiple ailments. Plant secondary metabolites such as alkaloids, tannins, steroids, terpenoids, coumarins and phenolic compounds and their semi-synthetic derivatives continue to play an important role in anticancer drug therapy. Molecular docking is a computational technique that aims to predict how a particular small molecule will stably bind to a target protein. It is an important component of many drug discovery projects when the structure of the protein is available. Although it is primarily used as a virtual screening tool, and subsequently for lead optimization purposes, there are also applications in target identification. To characterize the phytochemical constituents and anti-tumorigenic efficacy of methanolic extract of Ficus racemosa bark against cancer target proteins using in-silico Glide docking and QikProp panel. The results showed that the presence of phytochemical constituent’s namely, flavonoids, steroids, saponins, glycoside, tannins, phenolics, fixed oils and fats and the total 24 phytochemical constituents present...
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Posted by admin on Oct 31, 2019 in |
Medicinal plants are essential in our balanced life diet and are in demand in allopathic and herbal medicine. The present study was aimed to evaluate the phytochemical, antioxidant and antimicrobial activities of Phyllanthus emblica (Euphorbiaceae), Averrhoa bilimbi (Oxalidaceae) and Phyllanthus acidus (Euphorbiaceae) fruits. The preliminary phytochemical analysis was carried out using standard methods. Total phenolic, flavonoid, protein, vitamin-C and tannin content were analyzed. Antioxidant and antibacterial activity was assessed by DPPH and agar well diffusion method. Phytochemical screening revealed the presence of alkaloids, flavonoids, glycoside, phenols, proteins, and amino acids, steroids, saponins, terpenoids, tannins, resins and emodols in different fruit extracts. The phenolics, protein and vitamin C content were higher in Phyllanthus emblica fruits. While flavonoids and tannin content was higher in Averrhoa bilimbi fruits. The free radical scavenging activity was significantly higher in Phyllanthus emblica fruits. Maximum inhibition of antibacterial activity was noticed in Phyllanthus emblica against Bacillus subtilis followed by Klebsiella pneumoniae, Staphylococcus aureus, and Escherichia coli respectively. In conclusion, our studies provide evidence that fruit extracts...
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Posted by admin on Oct 31, 2019 in |
Diabetes mellitus is one of the most common endocrine disorder which causes microvascular and macrovascular complications. Herbal medicines derived from plant extracts are used to treat various diseases because of its fewer side effects. The present investigation includes a screening of phytochemicals and evaluation of the in-vitro and in-vivo anti-diabetic activity of Hellenia speciosa leaves extract. The leaf extract was screened for various phytochemicals and then subjected to in-vitro anti-diabetic study using alpha-amylase and alpha-glucosidase enzyme. Inhibition of carbohydrate digestive enzymes amylase and glucosidase can effectively decrease the postprandial increase of glucose level. This is an important strategy in the management of diabetes. This was followed by the in-vivo study to evaluate the anti-diabetic effect of Hellenia speciosa leaves in normal and alloxan induced diabetic rats. The oral administration of crude leaf extract in diabetic rats for 28 days at a dosage of 300 mg/kg body weight exhibited a significant reduction (P<0.05) in fasting blood glucose level and remarkable increases in serum insulin level. The action of Hellenia speciosa...
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Posted by admin on Oct 31, 2019 in |
Objective: To investigate the anti-proliferative effects of T. procumbens leaf extracts on the growth of MCF 7, Hep G2 and K562 cells. An attempt was made to partially purify the cytotoxic acetone extract using a bioassay-guided isolation procedure and the apoptosis-inducing activity of bioactive sub-fraction, F15 (fraction 15), was probed further against K562 cells. Methods: Cytotoxicity of extracts was evaluated by MTT assay. Hoechst 33258, acridine orange/ethidium bromide and AnnexinV- FITC staining was performed to detect apoptosis. Mitochondrial membrane potential and cell cycle distribution were measured by flow cytometry. DNA fragmentation, alkaline comet assays and Western blotting of key apoptosis-related proteins were also performed. Results: The F15 showed the highest antiproliferative activity against K562 cells leaving the normal peripheral blood lymphocyte unaffected. TLC of F15 displayed a total number of four bands that individually exhibited lower anticancer activities than that of F15, suggesting the possibility of synergism among them. F15 induced phosphatidylserine externalization, loss of mitochondrial membrane depolarization, DNA laddering, increased sub-G1 population and altered expression of caspase 3,...
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Posted by admin on Oct 31, 2019 in |
Industrial soil contains a variety of L-Asparaginase producing microorganisms such as bacteria and fungi. Microorganisms can produce different types of enzymes which are frequently used in different types of industries for different purpose. In the present study, we focused on isolation of L-Asparaginase enzyme producing bacteria from industrial soil samples collected from different regions of Gwalior. These bacterial isolates were Salmonella sp., Proteus vulgaris, Bacillus subtilis which were identified based on colony morphology, Gram staining, biochemical tests and using selective and differential media. All the tested isolates were able to produce enzymes like L-Asparaginase. All the three bacteria which were isolated from soils of a different region of Gwalior able to produce L-Asparaginase enzyme. Maximum L-Asparaginase activity was shown in second (II) bacterial isolate Salmonella sp., which was 12.734 mg/ml. Salmonella sp. were not reported to show any type of allergenicity when their sequences were checked by bioinformatic tool Algpred. So, these bacterial isolates are considered as a good source of enzyme production and may be used at an...
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