Posted by admin on Nov 30, 2019 in |
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for quantification of Darunavir in bulk and its tablet dosage form. The separation was carried out on Zorbax Eclipse XDB C18 (150 × 4.6 mm; 5 µm) column at 35 °C temperature using ammonium acetate buffer as mobile phase-A, acetonitrile (100%) as mobile phase-B. The flow rate was 1.0 ml/min and effluent was detected at 265 nm. The retention time of Darunavir propylene glycolate was 31.34 min. The percentage recovery was within the range between 95.46% and 100.17% for Darunavir propylene glycolate. The linear ranges were found to be 0.086-3.084 µg/ml (r2 = 0.9997) for Darunavir propylene glycolate. The percentage relative standard deviation for accuracy and precision was found to be less than 5.0%. Hence, the method could be successfully applied for routine analysis of Darunavir in pharmaceutical...
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Posted by admin on Nov 30, 2019 in |
The blood-brain barrier is the most challenging physiological obstacle for drug moieties for reaching the central nervous system. Meropenem is a drug used against meningitis, however crossing BBB is also a challenging realm of problem for the same. Therefore attempt has been undertaken to strategically design Meropenem loaded cholesterol-soya lecithin nanoliposomes (MCSLN). MCSLN was prepared by nano-emulsification technique with tween 80 as its surface ligand. Molecular docking studies revealed that tween 80 interacts with apolipoprotein E through π-π interactions and hydrogen bonding, which might be its mechanism for transmembrane permeation across BBB. After characterization, in-vitro release study was carried out to evaluate cross-walking efficiency of liposomal drug delivery system through the simulated blood-brain barrier. Entrapment efficiency estimation showed that about 80% of the drug has been successfully entrapped within the liposome. Dynamic light scattering study exhibited bimodal distribution of particle with size ranges 70-75 nm and 300-350 nm. Zeta potential reveals good stability of the formulation. FTIR studies reveal that Meropenem has been successfully entrapped within the formulation. On...
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Posted by admin on Nov 30, 2019 in |
As a part of our ongoing effect to develop a biodegradable medicated film for wound healing using chitosan and HPMC as polymers. The drug used is Silver sulfadiazine. This formulation produces better biodegradability and bioadhesion. The biodegradable film is non-toxic and non-irritant to the skin. An animal wound model was performed on the back of the rats and treated, respectively with medicated biodegradable film and with a marketed product. There are no infections found during the period of study. The wound healed within 6 days, in the case of medicated film formulation, but not with the marketed cream. Medicated films could be a good alternative to conventional wound dressings, which is painful and injurious. The film adheres to open wounds, and therefore it is good protection providing a moist healing environment. Better patient compliance is an added advantage as it does not require repeated...
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Posted by admin on Nov 30, 2019 in |
Objective: The objective of the study was to estimate the effects of Non-caloric Artificial Sweeteners (NAS) on glycaemic status in male Swiss albino mice. Methods: 36 male Swiss albino mice were selected and divided into six groups of 6 animals each. Group 1 served as control and received a normal pellet diet with drinking water. Groups 2, 3, 4, 5 and 6 received normal pellet diet and drinking water along with oral administration of Glucose, Saccharin, Sucralose, Aspartame, and commercial Aspartame, respectively for 8 weeks. Bodyweight of the animals was measured every week from baseline until the end of the experiment. Fasting blood glucose, serum glycated hemoglobin (HbA1c), serum insulin, Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), renal function tests, liver enzymes, fecal culture, and lipid profile were assessed during baseline and end of the study. Results: The bodyweight of the animals was increased in all groups. Fasting blood glucose and HbA1c were significantly increased in NAS groups. Saccharin group showed increase in serum insulin, HOMA-IR, liver enzymes, urea,...
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Posted by admin on Nov 30, 2019 in |
Nanoparticles are of great scientific interest, in fact, a bridge between bulk materials and atomic or molecular structures. The main characteristic of nanoparticles is their extreme smaller size, which falls in the transitional zone between molecules and the corresponding bulk materials. Lipids and fatty acids are a family of molecules classified within the lipid macronutrient class. The present study is based on the changes in lipid profile parameters due to Hg-Se NPs. Both mercury and selenium have a binding affinity for each other. Mercury is comparatively toxic than selenium; hence in combination, selenium works antagonistically to mercury. Acute and sub-acute treatments of mercury selenide nanoparticles (Hg-Se NPs) reflect changes in lipid profile in female Albino rats, which may be considered in cardiac risk assessment and its follow up program. The level of cholesterol, triglycerides (TG) and lipoprotein were found to be reduced after acute (1 day) and sub-acute (7, 14, and 21 days) treatments. The reduction in cholesterol and triglycerides revealed quick penetrating power of Hg-Se NPs whereas decrement...
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