Posted by admin on Nov 30, 2019 in |
Low level of oxygen in tissues leads to hypoxia, and hypoxia-inducible factors (HIF) regulate the hypoxic and normoxic conditions in various tissues. During normoxia, HIF-1α binds to von Hippel-Lindau E3 ubiquitin ligase complex that targets HIF-1α to the ubiquitin-proteasome pathway for proteolytic destruction. But in hypoxia, HIF-1α move to the nucleus where it binds to CBP/p300 at CH1 domain. By considering this fact, the present study was conducted to search a suitable inhibitor that can bind to HIF-1α. The unliganded HIF-1α was docked and the best five docking solutions complex were selected and analyzed by Ligplot. The analysis showed that catechin, epicatechin, myricetin, dicarnoxide D, and pycnidione had the maximum potential to inhibit HIF-1α protein and may prove to be potential inhibitor for counterfeiting hypoxic...
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Posted by admin on Nov 30, 2019 in |
The effect of a wide range of culture conditions on the production of laccase (Lac), Lignin peroxidase (Lip) and Manganese peroxidases (MnP) by Daedaleopsis confragosa was studied. A ligninolytic enzyme-producing bracket mushroom, Daedaleopsis confragosa was isolated from tree trunk from Karwar coastal region, Karnataka, India and subjected to production and dye decolorization study. Ligninolytic enzyme production was done by Submerged Fermentation (SmF). Various process parameters like different pH, temperature, carbon, nitrogen sources, and metal ions were investigated on Laccase (Lac), Lignin peroxidase (LiP) and Manganese peroxidizes (MnP) production in the fermentation process. The study revealed that the Lac, Lip and MnP highest activity was observed at pH 6.0, 5.5, and 7.2 respectively. Lac, Lip, and MnP showed highest activity at temperature 45°C, 40°C and 40°C respectively. Glucose as carbon source and modified PDB (Potato dextrose broth +2% saw dust + 1% yeast extract) as nitrogen source support maximum laccase activity. Starch as carbon source and modified PDB as nitrogen source enhanced the highest activity of Lignin peroxidase and Manganese...
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Posted by admin on Nov 30, 2019 in |
A reverse-phase high-performance liquid chromate-graphic method (RP-HPLC) was developed for the simultaneous estimation of combined dosage form of ezetimibe with simvastatin and rosuvastatin using RP-C18 column. For estimation of ezetimibe and simvastatin, the mobile phase (acetonitrile: water and pH adjusted to 4.5 with acetic acid) was pumped at a flow rate of 0.8 ml/min in the ratio of 85:15% v/v and the eluents were monitored at 234 nm. For the estimation of ezetimibe and rosuvastatin the mobile phase (acetonitrile: water and pH adjusted to 4.5 with acetic acid) was pumped at a flow rate of 0.8 ml/min in the ratio of 75:25% v/v and the eluents ezetimibe and rosuvastatin were monitored at 245 nm. Linearity was obtained in the concentration range of 2-12 μg/ml for ezetimibe in both the mobile phase, 2-12 μg/ml for simvastatin, and 2-12 μg/ml for rosuvastatin. The method was statistically validated, and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed method. Due to its simplicity,...
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Posted by admin on Nov 30, 2019 in |
A simple, sensitive and stability indicating UV spectrophotometric method was developed and validated for the estimation of caffeine in a gel formulation, plasma and urine samples using acidified alcohol as solvent. At absorbance maxima of 265 nm, the linearity was found to be in range of 4-20 µg/ml with correlation coefficient 0.9988. The method was applied for estimation of caffeine in a gel formulation, plasma, and urine samples and the results show excellent recoveries. The method was validated statistically and by recovery studies for linearity, precision, repeatability, and reproducibility. The caffeine was exposed to thermal, photolytic, hydrolytic, and oxidative stress conditions, and the stressed samples were analyzed using the proposed method to demonstrate the specificity of the method. The stated method can be used as a stability-indicating method with high degree of linearity, accuracy and precision for estimation of caffeine in gel formulation and biological...
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Posted by admin on Nov 30, 2019 in |
The aim of present work is to design a Tenoxicam loaded nanogel for the anti-inflammatory and analgesic effect of prepared nanogel. Tenoxicam is one of the NSAIDs of oxicam class; it is very potent and useful for chronic use in rheumatoid arthritis and osteriostatis. The formulations of nanogel based materials have high drug loading capacity, biocompatibility, and biodegradability which are the key points to design a drug delivery system effectively. This research article is to succinctly describe the recent development of nanogel drug delivery system concerning drug loading and swelling of drug from nanogel. To change the mode of administration of Tenoxicam for increasing its pharmacological action we prepared Tenoxicam loaded nanogel which is prepared by modified emulsification-diffusion method by using swelled polymer like noveon polycarbophil AA-1. The formulated nanogel was evaluated for particle size, zeta potential, drug content, entrapment efficiency, in-vitro drug diffusion studies, in-vitro permeation...
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