Posted by admin on Oct 31, 2019 in |
The aim of the present research was to develop and optimize novel expandable gastro retentive formulation of metronidazole to give local action for the management of Helicobacter pylori infection. The formulation contained a drug-loaded sandwich patch prepared by solvent casting method folded into a hard gelatin capsule. Metronidazole being needle shape crystals soluble in 0.1 N HCl, but after drying, the formulation show bursting effect in first 1 h. To avoid this drug-loaded middle layer containing Xanthan gum and HPMC E15 as release retardants was sandwiched between baking layers of HPMC E15. Drug loaded middle layer was subjected to 32 full factorial design with concentration of HPMC E15 (X1) and Xanthan gum (X2) as independent variables and release of the metronidazole at 1st h and at 4th h as Responses. According to 32 full factorial design 9 batches were prepared and evaluated for thickness, folding endurance, mucoadhesion, drug content and % drug release. The thickness of formulations F1 to F6 was found to be in the range of 0.266...
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Posted by admin on Oct 31, 2019 in |
A simple new rapid, accurate, robust, and specific HPLC method was developed for the assay of pantoprazole and domperidone from the oral solid dosage pharmaceutical formulations. The reverse-phase chromatographic method was developed on an RP C8 column (250 mm × 4.6 mm, 5 µm) using a mixture of 25 mM sodium dihydrogen phosphate solution of pH 6.8 and methanol in the ratio 40:60 v/v as mobile phase in an isocratic mode of elution at a flow rate of 1.0 ml/min at 35 ºC with a load of 20 µl. The detection was carried out at 286 nm. The method was validated concerning linearity, robustness, precision, accuracy, specificity & stability as per ICH guidelines. The method produced excellent separation with good linear correlation coefficients (≥ 0.999) for both the components. The proposed method could be successfully applied for the assay of pantoprazole and domperidone in the various oral solid dosages pharmaceutical formulations, namely tablets and capsules in the sustained release...
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Posted by admin on Oct 31, 2019 in |
The polyherbal combination was prepared by using aqueous and methanol root extracts of A. officinalis, B. diffusa, C. papaya, C. fistula, C. intybus, F. hispida, F. indica, C. nurvala, S. virgaurea and V. negundo in equal proportion for the dose of 25 mg/kg and 50 mg/kg body weight. Albino Wistar rats were selected for the study; the serum uric acid, urea, creatinine, BUN, and albumin level were measured using standard procedures. The elevated parameters in serum shows the statistically significant (**p<0.05) improvement with a low dose of aqueous extract combination i.e., hemoglobin (11.15 g/dl), CBC (5.93 ×103/µl), total bilirubin (0.61 mg/dl), total protein (09.85 mg/dl), AST (63.41 IU/L) & ALT (34.37 IU/L), uric acid (1.68 mg/dl), urea (57.06 mg/dl), creatinine (1.08 mg/dl), BUN (41.50 mg/dl) and albumin (40.84 mg/dl) in serum and urine. It was also reported as 24h urinary protein (2.96 mg/24h), albumin (09.23 mg/dl), urea (2.80 mg/dl), creatinine (38.28 mg/dl) and uric acid (126.08 mg/dl) shows significant improvement against disease control at the end of the study....
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Posted by admin on Oct 31, 2019 in |
The present study is a small attempt of synthesis of gold nanocomposites with anticancer drug cisplatin and their cytotoxicity test on HeLa cell line. There are many well-established reports on the synthesis of gold nanoparticles (AuNPs) chemically. But as an ecofriendly alternative various plant extracts also been used for the reduction and synthesis of AuNPs. AuNPs in this study have been synthesized from ethanolic bract extract of Musa balbisiana. Further the drug-loaded nanocomposite (AuNPCHCS) was synthesized using the synthesized AuNPs with chitosan (CH) and anticancer drug cisplatin (CS). AuNPs were tested for their cytotoxicity in a cancerous cell line, HeLa using MTT assay and found nontoxic up to the concentration of 100 µM. At the concentration of 10 µg/ml, cell viability reduced to 52% in case of nanocomposites and 55.7% in case of free drug. Results suggested that when nontoxic AuNPCH composites were loaded with CS, were able to kill the cancer cells efficiently compared to free drug. These synthesized nanoparticles are non-covalently conjugated with anticancer drug cisplatin. The...
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Posted by admin on Oct 31, 2019 in |
Objectives: The objectives of the study were to investigate the neurological, hematological, and histological effects of chronic exposure to nitrous oxide (N2O) in male Wistar albino rats. Methodology: 12 adults, male, Wistar albino rats (150-200g) were divided into 2 groups of 6 animals each. Group 1 served as control. Group 2 received N2O + O2 (70:30 mixture) for 1 h every day for 60 days. Neurological assessments were done by forced swim test, tail suspension test, actophotometer, rotarod, and elevated plus maze models at baseline and every 2 weeks, after that for 60 days. Complete blood count, liver function tests, renal function tests, and serum methemoglobin levels were assessed at baseline and on day 60. Rats were sacrificed on the 60th day, and histopathological examination of liver, kidney, spleen, and brain was done. Results: On comparing the results with the control group, the following changes were observed in nitrous oxide exposed rats. A: The duration of immobility was significantly reduced in forced swim test and tail suspension test, implying...
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