Posted by admin on Sep 30, 2019 in |
In ancient literature plant Alstonia scholaris (Apocynaceae) has been considered medicinally important in the treatment of various ailments. Extracts of this plant have shown wide spectra of pharmacological activities like anti-cancer, hepatoprotective, anti-inflammatory, anti-diabetic, etc. These activities can be attributed to the depositories of various phytoconstituents like alkaloids, flavonoids, phenols, triterpenoids, saponins, etc. present in it. Thus, a simple and precise HPTLC method has been developed for identification and quantification of a pentacyclic triterpenoid- lupeol in the leaves of Alstonia scholaris. The developed method was validated following ICH guidelines concerning linearity, range, specificity, precision, accuracy, LOD, and LOQ. After derivatization, the optimized mobile phase Toluene: Ethyl acetate: Formic acid (7:2:1 v/v/v) exhibited good resolution of bands at 580 nm. The Rf value of lupeol was found to be 0.63 and amount quantified in ethanol and chloroform extracts of leaves was 0.31 and 0.35% w/w respectively. The calibration curve of lupeol was linear between 200-1000 ng/band with correlation coefficient 0.9990. LOD and LOQ were 55 and 166.69 ng/band respectively. Recovery...
Read More
Posted by admin on Sep 30, 2019 in |
Rivaroxaban is utilized as a direct factor Xa inhibitor for the prevention and remedy of thromboembolic disorders. This study aimed to evaluate a generic version of rivaroxaban 10 mg tablet. Considering previous reports of safety and tolerability of a single dose (1.25-80 mg) of rivaroxaban, this study used a randomized, single-dose two-way crossover of rivaroxaban in 28 healthy volunteers, with a washout period of seven days. Analyses of blood samples were performed by a validated ultra-high performance liquid chromatography coupled with tandem mass spectrometry. An YMC-Pack ODS-AQ reversed-phase column with the mobile phase of acetonitrile and 10 mM ammonium acetate pH = 3 (70:30, v/v) at a flow rate of 0.3 mL.min-1 under isocratic elution was selected for the analysis. A range of 2.5-600 ng.mL-1 was obtained for the calibration curve. Pharmacokinetic parameters were calculated for the bioequivalence assessment. The results showed that two formulations have similar pharmacokinetics. The 90% confidence interval of the mean ratios of the test versus reference formulation of Ln transformed AUC0-30 (82.0-98.0), AUC0-inf (81.3-105.2),...
Read More
Posted by admin on Sep 30, 2019 in |
Peptic Ulcer Disease (PUD) is one of the most prevalent pathogenic conditions which affects around 5-10% of the global population. Helicobacter pylori infection and the use of Non-Steroidal Anti-inflammatory Drugs (NSAIDs) are two of the most common etiological causes in the PUD pathogenesis. First line treatment for PUD involves use of drugs which cause acid suppression or target against the eradication of Helicobacter pylori infection. Ulceration generally occurs due to the disturbance of the normal equilibrium caused by either enhanced aggression or diminished mucosal resistance. Peptic ulcer disease (PUD) may also occur as a result of the regular usage of certain drugs, irregular food habits, stress, alcohol consumption, and so forth. Recent years have shown a significant increase in the consumption of NSAIDs, despite their harmful side effects. However, administration of NSAIDs, along with certain drugs or supplements may reduce the deleterious side effects. This research evaluates the ulcer protective efficacy of the combined dose of Aloe vera and vitamin-E in the Indomethacin-induced ulcerated rats. The therapeutic efficacy of the combined...
Read More
Posted by admin on Sep 30, 2019 in |
This study was aimed to investigate the effects of different levels of dietary chromium picolinate (CrPic) on performance, some blood biochemical and intestinal morphology and microflora in Ross 308 broiler chicks. A total of 240 broiler chickens Ross 308 strain, from 21 to 42 days old were used in a completely randomized design. All chickens consumed a diet based on the corn-soybean meal, and the treatments were control- (thermo-neutral) and control + (heat stress) with no add-on and the others with CrPic supplementation at levels 500, 1000 and 1500 ppb respectively. The blood serum samples were subjected to biochemical analysis for blood parameters. The histomorphometric examination was performed by light microscopy. The result showed that all the growth and feed intake and feed conversion ratio parameters were declined when the broilers fed the CrPic supplementation (P≤0.05). The serum glucose concentration decreased CrPic supplemented groups. As a result, revealed that the villus height and crypt depth in the ileum and jejunum. Ileal villus height of birds on CrPic groups was...
Read More
Posted by admin on Sep 30, 2019 in |
Modified Benzoxazocine moiety of Nefopam with N-Protected-ethanolamine, fluorobenzene benzylbromide, 2,3-dimethyl-benzene, naphthalene, fluro-naphthalene. In these modification Friedel Craft alkylation plays vital role with using grignard reagent and aluminum chloride with the starting phthalic anhydride to form acid compound and was converted to amide compound with using of thionyl chloride, N-Protected ethanol amino and further converted to benzhydrol follows two synthetic routes, one routes is amide is converted to choro intermediate with thionyl chloride and then converted to benzhydrol, and another route is amide compound is directly converted into benzhydrol. Benzhydrol is the key intermediate for the preparation of all Nefopam analogs. These benzhydrol is undergone cyclization with p-Toluene sulfonic acid to forms benzoxazocine moiety of Nefopam analogs, by these ways disclosed nefopam analogs containing Benzoxazocine moiety as a novel analgesic therapeutic compounds, and these compounds were characterized by IR, 1H NMR Mass...
Read More
