Posted by admin on Oct 31, 2019 in |
Plant galls are remarkable close associations between plants and insects, in which the plant produces an abnormal growth of tissue in response to a specific stimulus from the attacking insect. The present investigation was undertaken to assess the antioxidant activity as well as the total phenols and ascorbic acid content in in-vivo (normal flower and galled flower) and in-vitro (unorganized static callus cultures) of Crataeva religiosa. Callus cultures (fifteen months old) were grown on MS medium supplemented with NAA (1.0 mg/l) and BAP (0.5 mg/l) using normal and galled flower explants. Antioxidant potential was found to be maximum (89.54%) in galled flower and minimum in normal flower callus (16.58%). Lower IC50 value indicates high antioxidant activity which was maximum in normal flower callus (456.12 ± 0.36) and minimum in galled flower (73.49 ± 0.24). Total phenols and ascorbic acid were also higher in galled tissues as compared to normal flower and in-vitro tissues. These primary findings showed that insect induces higher levels of phenolics and ascorbic acid constituents that...
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Posted by admin on Oct 31, 2019 in |
UPLC is a modern technique which refers to Ultra Performance Liquid Chromatography and enhances in three main areas: Speed, resolution, and sensitivity. In this present study, an accurate and precise UPLC method was developed and validated for the stability indicating assay method to estimate Tezacaftor and Ivacaftor simultaneously in both bulk and tablet dosage form. This method was developed using column HSS C18 (100 × 2.1mm 1.7m) with mobile phase 0.1% OPA Buffer: acetonitrile taken in the ratio 50:50. The flow rate was 0.3 ml/min with an 1 μl injection volume. The effluents were detected at a wavelength of 292 nm using the TUV detector. The method was validated concerning specificity, accuracy, linearity, precision, robustness. The correlation coefficient for Tezacaftor and Ivacaftor were found to be 0.999 and 0.999, respectively. Recovery of Tezacaftor and Ivacaftor in the formulation was found to be 99.97% and 99.65%...
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Posted by admin on Oct 31, 2019 in |
The main objective of the study was to enhance the dissolution of fenofibrate (BCS class II), poorly soluble drug. To improve the solubility of the drug, solid dispersions were prepared by dropping method with PEG 4000 and PEG 6000 in the ratios of 1:1 and 1:2. The formulations were filled into capsules and evaluated for solubility, assay, FTIR, X-ray diffractions, DSC, and in-vitro dissolution. The optimized formulation was compared with the marketed formulation. The optimized formulation D4 (containing PEG 6000 (1:2) by dropping method) showed maximum solubility 0.678 ± 0.07 mg/ml when compared to pure drug (0.018 mg/ml), assay, 98.14 ± 12% and practical percentage yield 95.25 ± 0.17%. In-vitro dissolution studies of the prepared solid dispersions showed release in 60 min whereas D4 formulation released 99.10 ± 0.18% in 30 min when compared to pure drug 27.38 ± 0.10% in 60 min, and a marketed capsule containing micronized fenofibrate (Lipicard) 93.91 ± 0.12% in 30 min. FTIR studies confirmed that there is no interaction between the drug and...
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Posted by admin on Oct 31, 2019 in |
Objectives: To retrospectively determine the prescription pattern of antiepileptic drugs (AEDs), hematobiochemical changes, and associated adverse effects. Methodology: A retrospective observational study was conducted at the Department of Neurology, King Fahad Hospital of the University, Khobar, KSA. Data Collection was through Electronic Health Record. A designed validated form to record demographic characteristics, hematological-biochemical parameters and adverse effects were utilized. Results: 115 patients were recruited. 54 (44%) were female and 64 (56%) were male. Carbamazepine; CBZ (n = 47; 40.9%) and Valproic acid; VPA (n = 32; 27.8%) were the most frequently prescribed antiepileptic drugs (AEDs). While CBZ + VPA (n = 21, 18.3%) was the most frequently prescribed combination. Generalized seizures were more frequently diagnosed (n = 80, 70%) compared to partial seizures (n = 22, 19%). The hematological abnormalities manifested as reduction in levels of Hemoglobulin (Hg), Hematocrit, Mean corpuscular volume (MCV), Mean corpuscular hemoglobin (MCH) with all AEDs, while CBZ treatment was additionally associated with reduction in Mean corpuscular hemoglobin concentration (MCHC). The combination of CBZ +...
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Posted by admin on Oct 31, 2019 in |
Diabetes mellitus is a lasting metabolic disability regarding enhance etiology and permanent degenerating complications. Sexual abnormalities (Hypogonadism, infertility, etc.) are outfit a two of the discombobulate ingenious stringency of diabetes mellitus. Different hypoglycemic agents are used to control diabetes mellitus, but very few formulations are available to treat secondary complications. Results of the present study establish the anti-diabetic properties of Camellia sinensis and its impact on the male reproductive systems of the streptozotocin-induced diabetic male...
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