Posted by admin on Oct 31, 2019 in |
Diabetes mellitus is a major endocrine metabolic disorder of multiple etiologies characterized by chronic hyperglycemia; with disturbances of carbohydrate, protein, and fat metabolism. It is caused by the deficiency or ineffective production of insulin by pancreas, which results in increase or decrease in concentrations of glucose in the blood. There is number of chemical agents available to control and to treat diabetic patients, but total recovery from diabetes has not been reported till date. An alternative to these synthetic agents, many herbal plants with hypoglycaemic properties are known from across the world. The World Health Organization (WHO) has listed 21,000 plants, which are used for medicinal purposes around the world. The aim of the present study was to determine the anti-diabetic activity of the hydroalcoholic extracts of bark of Anacardium occidentale, whole aerial part of Achyranthes aspera and leaf of Aegle marmelos. Wistar rats were made diabetic by a single dose of alloxan monohydrate (120 mg/kg, i.p.). Hydroalcoholic extracts were screened for antidiabetic activity in alloxan-induced diabetic rats at a...
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Posted by admin on Oct 31, 2019 in |
Objective: This study was an attempt to explore the latent of colon specificity approach utilizing pH-sensitive and time-dependent characteristics of polymers for defined colonic release of 5-Fluorouracil (5-FU). Method: The pellets were prepared by extrusion-spheronization method which is an industry-accepted method due to its ease of formulation. The prepared pellets were the matrix of 5-Fluorouracil with pH-sensitive and time-dependent polymers, i.e. Eudragit FS30D and Eudragit NM30D respectively. The changing pH media used for in-vitro release study of optimization batches for both the polymer concentrations. The Optical microscopy and Scanning electron microscope (SEM) was used to evaluate texture and surface morphology. Results: The t10%, t30%, t50%, and t90% values for optimized formulation were found as 2.7 h, 6.2 h, 8.7 h and 18.2 h respectively. These values were extrapolated from in-vitro release vs. time plot which also indicated that the required lag time of 6-8 h was achieved. The in-vivo roentgenography or X-ray imaging study was used to confirm the lag time and transition path of colon targeted matrix pellets....
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Posted by admin on Oct 31, 2019 in |
Simple, sensitive, and rapid stability indicating RP-HPLC method for simultaneous estimation of Pantoprazole and Levosulpiride in pharmaceutical dosage form was developed and validated. The analysis was carried out on Hibar C18 column (250 × 4.6 mm, id, 5µ) and the mobile phase composition was 10 mM ammonium acetate (pH 4.0 adjusted using acetic acid): Acetonitrile in the ratio of 20:80% v/v with a flow rate of 1.0 mL/min at room temperature. The sample injection volume was 20 µL, and eluents of the isocratic elution mode were monitored at 241 nm. The retention time was 3.1 min and 5.2 min for Pantoprazole and Levosulpiride, respectively. The method was linear in the concentration range of 1-7 μg/ml for Pantoprazole sodium with an r2 of 0.9973 and 4-10 µg/ml of Levosulpiride with an r2 of 0. 9961. The LOD and LOQ were found to be 0.05 and 1.5 µg/ml for Pantoprazole and Levosulpiride. The drug stability was assessed under various stress degradation conditions at room temperature for 24 h. In photodegradation, the...
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Posted by admin on Oct 31, 2019 in |
Plant galls are remarkable close associations between plants and insects, in which the plant produces an abnormal growth of tissue in response to a specific stimulus from the attacking insect. The present investigation was undertaken to assess the antioxidant activity as well as the total phenols and ascorbic acid content in in-vivo (normal flower and galled flower) and in-vitro (unorganized static callus cultures) of Crataeva religiosa. Callus cultures (fifteen months old) were grown on MS medium supplemented with NAA (1.0 mg/l) and BAP (0.5 mg/l) using normal and galled flower explants. Antioxidant potential was found to be maximum (89.54%) in galled flower and minimum in normal flower callus (16.58%). Lower IC50 value indicates high antioxidant activity which was maximum in normal flower callus (456.12 ± 0.36) and minimum in galled flower (73.49 ± 0.24). Total phenols and ascorbic acid were also higher in galled tissues as compared to normal flower and in-vitro tissues. These primary findings showed that insect induces higher levels of phenolics and ascorbic acid constituents that...
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Posted by admin on Oct 31, 2019 in |
UPLC is a modern technique which refers to Ultra Performance Liquid Chromatography and enhances in three main areas: Speed, resolution, and sensitivity. In this present study, an accurate and precise UPLC method was developed and validated for the stability indicating assay method to estimate Tezacaftor and Ivacaftor simultaneously in both bulk and tablet dosage form. This method was developed using column HSS C18 (100 × 2.1mm 1.7m) with mobile phase 0.1% OPA Buffer: acetonitrile taken in the ratio 50:50. The flow rate was 0.3 ml/min with an 1 μl injection volume. The effluents were detected at a wavelength of 292 nm using the TUV detector. The method was validated concerning specificity, accuracy, linearity, precision, robustness. The correlation coefficient for Tezacaftor and Ivacaftor were found to be 0.999 and 0.999, respectively. Recovery of Tezacaftor and Ivacaftor in the formulation was found to be 99.97% and 99.65%...
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