Posted by admin on Oct 31, 2019 in |
The powder date palm cake is sold in coffee and confectionary shops in the central region of the Kingdom of Saudi Arabia, where it is used as a hot drink in the form of decoction. It is alleged by users in the local community in Saudi Arabia to improve fertility. The date palm cake is the roasted grounded seeds of the date palm (Phoenix dactylifera L.). The objective of this study is to evaluate the potential protective effect of the date palm extracts against nickel chloride-induced infertility in male mice. The effects of nickel compounds on reproduction in rodent models are well documented. The method adopted is the injection of nickel chloride (10 mg/kg) intra-peritoneal to induce infertility in male mice. The chloroform and ethyl acetate extracts were used to assess their protective effects. Sperm abnormalities and testicular histopathology were evaluated. The results showed that the administration of chloroform extract enhanced the sperm count and reduced sperm abnormalities. Moreover, the abnormalities induced by nickel chloride were restored to a...
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Posted by admin on Sep 30, 2019 in |
The leading factor restraining the intensification of herd reproduction is endometritis. The article states that the microflora of cow uterine exudate in chronic purulent-catarrhal endometritis is represented by Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Citrobacter freundii, Streptococcus faecalis, Streptococcus faecium, as well as anaerobes and group С Streptococci. The drug “Mitreс” showed the greatest effectiveness of suppressing the growth of the majority of isolated strains of microorganisms. Its zone of growth inhibition was as follows: Streptococcus faecium – 31 ± 2.06 mm, Citrobacter freundii – 30 ± 0.00 mm, anaerobes – 29 ± 0.00 mm, Proteus vulgaris – 28.5 ± 1.72 mm, Escherichia coli – 27.0 ± 1.45 mm; group C Streptococci– 27.32 ± 2.12 mm; Staphylococcus aureus strains- 25.5 ± 1.66 mm; Streptococcus faecalis– 25.0 ± 1.12 mm. The recovery of cows with chronic purulent-catarrhal endometritis after applying “Tilozinocarum” was 80%, “Endometramag-T” showed 90%, and mitrec demonstrated 100% recovery. During the 90 days of observation of the experimental group of cows injected with the drug “Sepranol” fertilization occurred...
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Posted by admin on Sep 30, 2019 in |
Objective: State of the art, robust and environmentally benign stability indicating assay method (SIAM) has been developed for model drug Edoxabantosylate (EDO) Methods: Successful RP-HPLC chromatographic method was accomplished on a CHROMBUDGET® 100-5-C18 column (250 mm × 4.6 mm, 5 μ) column using methanol and acetate buffer in the ratio of 53:47 having 2.9 pH and 10mM buffer strength. The degradation study was performed in the presence of various stressors, and SIAM develop was selective enough to discriminate the EDO peak with the peak of other degradation production (DP) with acceptable resolution. The degradation kinetics study and fragmentation pattern of isolated major DPs were carried out. Results: The developed SIAM validated as per ICH Q2(R1) guidelines. Two major DPs i.e. DP-I and DP-II, were isolated and characterized thoroughly. Furthermore, the reaction kinetics data revealed that EDO degradation was following first-order degradation kinetics. Conclusion: An extensive stress degradation study along with degradation kinetics, were performed. All probable degradation pathways were predicted by using LC-MS/MS and NMR data obtained by characterization...
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Posted by admin on Sep 30, 2019 in |
This study was performed to identify the antimicrobial compounds in Abies webbiana leaves. The antimicrobial compounds were identified by LCMS-MS. The microbial inhibitory activity was evaluated on Staphylococcus aureus ATCC 6538 using REMA technique and Agar-overlay TLC bioautography. The chloroform extract of Abies webbiana leaves was used for the study. The MIC against Staphylococcus aureus was found to be 195.3 µg/ml- 390.6 µg/ml. A mobile phase comprising of toluene: ethyl acetate: glacial acetic acid (9:1:0.3) was used for identification of antimicrobial compounds present in the extract by TLC bioautography. Totally 16 bands were observed in the HPTLC chromatogram when the plate was observed at 254 nm. The band observed at Rf = 0.58 showed the antimicrobial activity. Preparative TLC technique was used to isolate this active band. The compounds present in the isolated band were then identified by LC-QTRAP. Totally 7 compounds were identified to be present in the isolated bands of chloroform extract. The compounds identified include Betuloside, 2,7-Dihydroxy-4’-methoxyisoflavone, Geinstein 7-O-beta-D-glucoside, β-Sitosterol, Abietane, Coniferol, and 1-(3,4-Dihydroxyphenyl)-1-decene-3,5-dione-Pos. This study...
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Posted by admin on Sep 30, 2019 in |
Schiff bases of isatin are investigated overtime for their pharmaceutical properties and have been found to have various activities such as anti-viral, anti-bacterial, anti-inflammatory, analgesic, anti-HIV, anti-depressant, anti-convulsant, fungicidal, etc. Isatins are treated with hydrazine derivatives and cyclized by sulfuric acid. These compounds are alkylated by di alkyl aminoalkyl halides in specific catalysed conditions. In this study we focus on 4-([1,3,4]oxadiazino[6,5-b] indole-3-yl)aniline derivatives for anti-bacterial activity. Based on literature, glucoseamine-6-phosphate synthase (PDB ID: 2VF5) and dihydropteroate synthase (PDB ID: 1AJ0) enzymes are chosen as potential targets as showed activity for antibacterial and antifungal agents. The in-silico studies were carried out using Swiss ADME and Molinspiration in-silico tools and scoring of molecular docking i.e., virtual screening was done by using AutoDock v.4.0. The in-vitro studies were carried out in the lab using cup-plate method and the zone of inhibition (in mm) was measured. The in-silico results were compared with that of in-vitro and a comparison was drawn. The activity of the compounds was found to have...
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