Posted by admin on Jun 30, 2019 in |
Green tea represents a popular beverage worldwide due to its high polyphenol and antioxidant activity. It is commonly brewed along with vitamin C source which plays a positive role in maintaining its antioxidant potential. Orange juice can serve as an excellent medium for supplementation of green tea for the formulation of nutraceutical nectar by enriching drink with vitamin C and overcoming the astringent taste of tea. Therefore, the present study was planned to develop nutraceutical nectar by blending green tea with orange juice at 60, 50, 40 and 30% levels while orange juice and green tea served as control. The formulations were analyzed for their sensory as well as nutritional properties. Among all the samples, the highest values for ash content, ascorbic acid content, titratable acidity, and TSS were recorded for orange juice. Green tea displayed the highest values for total flavonoid content, antioxidant activity (FRAP, DPPH, TEAC), moisture content and pH compared to other blends. Blending green tea with an increasing concentration of orange juice resulted in a...
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Posted by admin on Jun 30, 2019 in |
Oxidative stress has been recommended as a contributory factor in complication and progression of diabetes. The aim of the study was to evaluate the effects of Rhizophora mucronata Leaves on streptozotocin generated oxidative stress induced diabetic nephropathy in rats. Forty Wistar rats were randomly divided into normal control, diabetic untreated and diabetic treated with ethanolic extract of Rhizophora mucronata leaves (ERML) at 250 and 500 mg/kg doses, respectively. Experimental diabetic nephropathy was induced by a single intraperitoneal (i.p) administration of STZ 60 mg/kg body weight (b.w.). The body weight, plasma glucose, creatinine, urea, uric acid as well as total protein along with oxidative stress parameters were investigated in various groups of rats. Histopathological analyses were also conducted on the kidney tissues. In STZ treated rats LPO were increased whereas the level of SOD, CAT and GSH were decreased significantly. ERML at 250 and 500 mg/kg, in a dose-dependent manner, significantly improve all levels as compared to diabetic rats. Destruction of the liver architecture of the hepatocytes in the diabetic...
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Posted by admin on Jun 30, 2019 in |
Type 2 diabetes mellitus is associated with cognitive impairment. The antidiabetic treatment found to delays the impairment. However, the benefit is due to antidiabetic drugs, or control of blood sugar is debatable. Most of the drug-based studies used monotherapy, which contradicts the clinical practice. This study aims to assess cognitive function in type-2 diabetes mellitus patients on metformin and other oral hypoglycaemic agent combination treatment. The cross-sectional study included 325 type 2 diabetes patients based on inclusion and exclusion criteria. They were categorized based on a combination of oral antidiabetics. Socio-demographic and medication data were obtained using medical records and personal interviews. Mini-Mental State Examination Scale- Tamil version (MMSE) was applied to measure the cognitive functions. Aged patients showed a reduced MMSE score of 18.18 ± 1.34. Increased duration of diabetes, >15 years, had the least MMSE score of 21.36 ± 1.37. Metformin-DPP-IV inhibitors combination found to benefit the cognition MMSE score = 29.11 ± 0.19 (P<0.001), compared to alpha glucosidase-metformin and thiazolidinedione- metformin combinations. A similar observation was...
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Posted by admin on Jun 30, 2019 in |
The present study was proposed to delineate the curative role of arjunolic acid and vitamin B12 alone or in combination against arsenic induced oxidative stress and female reproductive dysfunction. The Wistar strain adult female rats were treated with sodium arsenite at the dose of 1.0 mg/100 gm body weight orally for first eight days. Next eight days rats were treated with arjunolic acid (1.0 mg/100 gm body weight) and vitamin B12 (0.09 μg/100 gm body weight) orally. In ovarian and uterine tissue showed a recovery in arsenic induced ROS-production following the post-treatment of arjunolic acid and vitamin B12. Antioxidant status was also improved by arjunolic acid and vitamin B12 as established from the electrozymographic analysis of SOD, catalase and GPx. Post-treatment of these two significantly retrieved ovarian estradiol receptor 1 (ESR-1) from arsenic induced down regulation. Arjunolic acid and vitamin B12 also showed its curative ability in improving the level of inflammatory markers such as NF-κB, metallothioneine-1 (MT-1), TNF-α, IL-6. Thus these two bioactive substances might have the curative...
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Posted by admin on Jun 30, 2019 in |
A simple, rapid, and sensitive high-performance liquid chromatographic method with UV detection has been developed and validated according to the ICH guidelines for the quantitation of Cetirizine Hydrochloride (CET) and Phenylephrine Hydrochloride (PHEN) in the tablet dosage form. Chromatographic separation was carried out in a Zorbax eclipse plus, C18 column (250 mm × 4.6 mm; 5 µm particle size) with simple mobile phase composition of a mixture of Buffer (Water + 0.2% v/v Triethylamine, pH 7.5 by dil. H3PO4) and Acetonitrile (63:37 v/v) at a flow rate of 1ml/min and sample injection of 20 µL was injected at 25 ºC. The eluent was monitored with a UV/VIS detector set at 232 nm with a total run time of 15 minutes. The method was linear over the concentration range of 20-100 µg/ml for CET and 40-100 µg/ml for PHEN with a correlation coefficient of 0.999 for both the analytes. Accuracy and precision values of both within-run and between-run were obtained from six different sets of three quality control (QC) samples...
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