Posted by admin on Sep 30, 2019 in |
The microbial pigments have more applications than synthetic pigments and are easily biodegradable and safe to use. Among all bacteria, the anoxygenic phototrophic purple non-sulfur bacteria have more applications and can synthesize different pigments. In this present study, bacterial carotenoids were isolated from the two novel strains of Rhodopseudomonas palustris and evaluated its applications. The antibacterial activity was studied by zone inhibition method. The pigment A1 and A2 showed more antibacterial activity against gram-negative and gram-positive bacteria. In-vitro antioxidant activity of bacterial pigments and bacterial extracts were evaluated by DPPH assay. The pigment A2 and Rp. KRPR02 showed more radical scavenging activity. The percentage inhibition of DPPH radical for pigment A2 (82.74 ± 4.49) and bacterial extract of Rp. KRPR02 bacteria (83.33 ± 5.46) is almost nearer to the percentage inhibition of DPPH radical for standard (ascorbic acid) which is 96.41 ± 1.81. In-vitro cytotoxic activity was studied by MTT assay and found that the pigments showed cytotoxic activity against the HeLa cell line, DU145 cell line, MCF7 cell...
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Posted by admin on Sep 30, 2019 in |
In the present study, the pharmaceutical and therapeutic dimensions of Shankha Bhasma (Classical Ayurvedic marine medicine) is explored as a substitute for calcium supplements available in the market. Shankha Bhasma was prepared & standardized by Ayurvedic pharmaceutical procedure accordingly in Rasa Tarangini (a classical 20th-century book on Ayurvedic pharmaceutical medicine). Conch shell (Shankha) was subjected to bio-fomentation (Swedana) in a precise heating container (Dolayantra) containing lemon juice for 12 h. Further, triturated with Aloe vera juice (Kumari Swarasa) for bio-impregnation (Bhavana) and subjected to the controlled quantum of heat (Marana) in the sealed earthen saucer at 700 °C for 2 h, till all the classical and contemporary quality parameters about bio-metallic medicinal powder (Bhasma) was assured. On completion, average weight loss was 17-21%. The final product obtained was calcite form of CaCO3 and calcium oxide hydrate (CaO.H2O). This paper explores scientific evidence of ancient Ayurvedic pharmaceutical procedures in terms of bio-fomentation, bio-impregnation, transfer mechanism of quantum of heat along with the principles of fluid mechanics &...
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Posted by admin on Sep 30, 2019 in |
The objective of the present study was to formulate and evaluate a gastro-retentive stomach specific novel floating in-situ gelling system of curcumin for potentially treating gastric ulcer, associated with Helicobacter pylori. The in-situ gel of curcumin was prepared by dissolving different concentrations of gelling polymer like sodium alginate in distilled water at 60 ºC. After cooling to 40 ºC, required quantities of sodium citrate and calcium carbonate were dispersed in it with continuous stirring followed by the addition of curcumin and sorbitol. Identification of drug was confirmed by DSC (melting point study), FTIR (functional groups study), and UV spectrophotometric analysis. Compatibility between drug and polymer were confirmed by DSC and FTIR studies. The micromeritic properties of curcumin were done, and all formulations showed pH in the range of 6.6 to 7.4, floating lag time was less than 1 min, duration of floating was more than 24 h for all the prepared formulations. Gelling capacity, gel strength, viscosity, and water uptake by the gel increased with the increase in sodium...
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Posted by admin on Sep 30, 2019 in |
The objective of the present study was to develop and validate a novel RP-HPLC method for the determination of Dasatinib in the pharmaceutical dosage form. Chromatographic separation was conducted on agilent technologies-1260 series with the G1311C quaternary pump, Thermo Scientific C18 column (4.6 mm i.d. × 250 mm, 5 µm particle size) and equipped with photodiode array detector G1315D. The mobile phase consisted of methanol and acetonitrile mixed in the ratio of 50:50 v/v, was used at a flow rate of 1 ml/min, and the detection wavelength was set at 323 nm. The retention time for Dasatinib was found to be 4.073 min. The calibration was linear (r2 = 0.999) in the concentration range of 2-10 µg/ml. The limit of detection and the limit of quantitation were found to be 0.5263 μg/ml and 1.5948 µg/ml, respectively. Recovery of Dasatinib in tablet formulation was observed in the range of 98.09-99.57%. Percentage assay of Dasatinib (Dasanat) was found to be 99.45% w/w. Thus the novel proposed method for Dasatinib was found...
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Posted by admin on Sep 30, 2019 in |
The elected work reports for the first time successfully synthesized nanosilver from the aqueous leaf extract of the Carchorus hirsutus plant. Because the phytochemicals within the leaf extract were creditworthy for the reduction of silver ions as the active nanosilver particles, in addition to this, they act as the capping agents who had been environed at the surface of the particles. UV-Vis spectroscopy oversaw the establishment of nanosilver as an appropriate peak detected at 412 nm of surface plasmon resonance has affirmed the colloidal nanosilver. The peaks of Fourier Transform Infrared Spectroscopy had proved to be phytochemicals responsible for the reduction of nanosilver. The crystalline structure and average diameter found to be 29 nm of the phytosynthesized nanosilver particles were found out using X-ray diffraction studies. The Scanning Electron Microscopy images were rendered the aggregated, semi-spherical to spherical silver nanocrystals. The elemental signals of energy dispersive X-ray spectrum substantiate the presence of silver detail as silver weight % is 86.84 and atomic % is 68.45. The atomic force microscope...
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