Posted by admin on Jun 30, 2019 in |
Simple, sensitive and selective methods are developed for the spectrophotometric determination of drugs, viz., Cetirizine hydrochloride, Duloxetine hydrochloride, Esmolol hydrochloride, Gemifloxacin, Moxifloxacin and Phenylephrine Hydrochloride based on their reactivity towards Chloramine-T (ChL-T). The method is based on the oxidation of drugs by ChL-T (Excess amount) in presence of acidic medium and estimating the amount of un-reacted ChL-T by Safranin-O (SO) dye at λmax 524 nm. The amount of ChL-T reacted is equal to the drug concentration in this method. The concentration of ChL-T is 10 µgmL-1 and Safranin-O Dye is 10 µgmL-1. The calibration curves obeyed Beer’s law over the concentration range of 2.5-17.5 µgmL-1 (CET), 5-35 µgmL-1 (DUL), 12-84 µgmL-1 (ESM), 10-70 µgmL-1 (GEM) & 2.5-17.5 µgmL-1 (MOX). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. The method has been validated in terms of guidelines of International Conference on...
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Posted by admin on Jun 30, 2019 in |
The present investigation was carried out the effect of petrochemical effluent on hematological parameters in freshwater fish, Labeo rohita using the standard method. The petrochemical effluent has the potential to cause developmental effects in fish. Blood offers important profile to study the toxicological impact on animal tissues. Hematology is used as an index of fish health status under different stress conditions on exposure to pollutants. The fish Labeo rohita was exposed to sub-lethal concentrations (30 ml/L) of petrochemical effluent for different h (24, 48, 72 and 96) and parameters like RBC, WBC, HB, MCV, MCH MCHC, PCV has been analyzed. All hematological parameters except WBC were found to be decreased from control, and the WBC was increased in all exposure periods indicating that test fish, Labeo rohita suffered hemolytic anemia and leucocytosis. Increase in time produced a declining value of RBC, Hb, MCV, and MCH also exhibit the sub-lethal concentrations of the petrochemical...
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Posted by admin on Jun 30, 2019 in |
The current work is intended towards the development of a simple, rapid and precise UV-Visible spectrophotometric method for the simultaneous estimation of Atorvastatin (ATR) and Clopidogrel (CP) present in the marketed formulation using UV simultaneous equation. Methanol was used as a solvent and the absorbance of the drug was measured at the absorbance maxima (λmax) of Atorvastatin & Clopidogrel, UV 246 nm & 202 nm respectively. Calibration curve plotted in concentration range 5-25 μg/mL exhibited an excellent linear relationship for both Atorvastatin and Clopidogrel. The method was found to comply all the validation parameters as per the ICH guideline, such as accuracy (mean recovery = 99.97%), precision, and linearity (r2 = 0.9934). The method showed good reproducibility and recovery with % RSD in the favorable range indicating the sensitivity of the method towards analyte. This method can be effectively employed for the day to day analysis of both drugs in the capsule dosage...
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Posted by admin on Jun 30, 2019 in |
Lambda-cyhalothrin [-cyano-3-phenoxybenzyl 3-(2-chloro-3,3,3-trifluoroprop-1-enyl)-2,2-dimethylcyclopropanecarboxylate] is one of the type II synthetic broad-spectrum pyrethroids used to protect the crops against insects. The aim of the present study was to evaluate the protective effect of resveratrol on male reproductive health in lambda-cyhalothrin exposed rats. Exposure of male rats to lambda-cyhalothrin resulted in a significant reduction in the reproductive organ weights accompanied by a reduction in the testicular daily sperm count and epididymal sperm count, sperm motility, and sperm viability. Further, a significant decrease in the activity levels of superoxide dismutase and catalase with a significant increase in the levels of lipid peroxidation were observed in the testis of lambda-cyhalothrin administered rats over the controls. Moreover, the integrity of testicular architecture was deteriorated in lambda-cyhalothrin exposed rats. Conversely, supplementation of resveratrol enhanced the activity levels of testicular enzymatic antioxidants and inhibited lipid peroxidation levels in lambda-cyhalothrin exposed rats as compared to its respective controls. Significant increase in the selected epididymal sperm variables accompanied by the restoration of testicular architecture was recorded in resveratrol...
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Posted by admin on Jun 30, 2019 in |
Deoxyelephantopin and isodeoxyelephantopin are member of a group of compounds, sesquiterpene lactones, that include proven anticancer agents against many types of cancer cells. Many sesquiterpene lactones suppress cancers by inhibiting NFκβ. This study explores the inhibitory interaction between deoxyelephantopin and isodeoxyelephantopin on NFκB using QSAR and molecular docking. Physicochemical properties and NFκβ (pIC50) inhibition values of a number of sesquiterpene lactone compounds from in–vitro studies were used as reference data in constructing the QSAR model equation. This model was used to predict the inhibition potential of deoxyelephantopin and isodeoxyelephantopin on NFκβ. This result was confirmed with molecular docking. The potential NFκβ inhibition of deoxyelephantopin and isodeoxyelephantopin obtained were 59.1037 μM and 62.0321 μM, respectively, with RMSE = 0.07831 R2 = 0.95465 and Q2 = 0.67719. Both test compounds showed affinity for the Lys 122 receptor residue (PDB ID: 1NF1) with a docking score of -9.5318 kcal/mol and -8.4429 kcal/mol,...
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