Posted by admin on Oct 31, 2019 in |
The present study is a small attempt of synthesis of gold nanocomposites with anticancer drug cisplatin and their cytotoxicity test on HeLa cell line. There are many well-established reports on the synthesis of gold nanoparticles (AuNPs) chemically. But as an ecofriendly alternative various plant extracts also been used for the reduction and synthesis of AuNPs. AuNPs in this study have been synthesized from ethanolic bract extract of Musa balbisiana. Further the drug-loaded nanocomposite (AuNPCHCS) was synthesized using the synthesized AuNPs with chitosan (CH) and anticancer drug cisplatin (CS). AuNPs were tested for their cytotoxicity in a cancerous cell line, HeLa using MTT assay and found nontoxic up to the concentration of 100 µM. At the concentration of 10 µg/ml, cell viability reduced to 52% in case of nanocomposites and 55.7% in case of free drug. Results suggested that when nontoxic AuNPCH composites were loaded with CS, were able to kill the cancer cells efficiently compared to free drug. These synthesized nanoparticles are non-covalently conjugated with anticancer drug cisplatin. The...
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Posted by admin on Oct 31, 2019 in |
Objectives: The objectives of the study were to investigate the neurological, hematological, and histological effects of chronic exposure to nitrous oxide (N2O) in male Wistar albino rats. Methodology: 12 adults, male, Wistar albino rats (150-200g) were divided into 2 groups of 6 animals each. Group 1 served as control. Group 2 received N2O + O2 (70:30 mixture) for 1 h every day for 60 days. Neurological assessments were done by forced swim test, tail suspension test, actophotometer, rotarod, and elevated plus maze models at baseline and every 2 weeks, after that for 60 days. Complete blood count, liver function tests, renal function tests, and serum methemoglobin levels were assessed at baseline and on day 60. Rats were sacrificed on the 60th day, and histopathological examination of liver, kidney, spleen, and brain was done. Results: On comparing the results with the control group, the following changes were observed in nitrous oxide exposed rats. A: The duration of immobility was significantly reduced in forced swim test and tail suspension test, implying...
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Posted by admin on Oct 31, 2019 in |
Wellness is described as positive health of an individual as indicated by the quality of life and a sense of well-being. This research aimed to assess the effect of supervised fartlek training on Perceived Wellness of University Students of Saudi Arabia. A sample of forty students enrolled in various courses of KFUPM was selected for the study; the mean age was 18.52 ± 0.599 years. Two groups were formed randomized into intervention (n=20) employed 16 weeks of supervised whistled fartlek training program and control group (n = 20) carried out their daily task and did not participate in any exercise program. The training session was conducted twice a week, and the duration of each session lasted for 45 min. All students were administered the Perceived Wellness Survey to assess their wellness. Subjects involved in regular physical activity for last 6 months were included for the study; obese students (BMI≥ 30 kg/m2) and who are on serious illness and medication, suffered cardio-vascular problems were excluded. At baseline demographic data for...
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Posted by admin on Oct 31, 2019 in |
Many young men, women, and even the elderly are addicted to Cannabis intake abuse despite its predictable toxicological consequences. In this paper, we studied the toxic effects of oral administration of methanol extract of Cannabis sativa seeds using a total of forty male Wistar Rats. Animals were randomized into five groups (n = 8 rats) of approximately equal weight. Group 1 received 100 mg/kg of the extract, group 2 received 200 mg/kg of the extract, group 3 received 300 mg/kg dosage of the extract, group 4 received 2 ml of olive oil and group 5 received distilled water for 14 days. Result for AST was significantly (p<0.05) higher in groups 2 (57.00 ± 13.00 IU/L) and 3 (59.33 ± 10.53 IU/L), compared with normal control group 5 (31.33 ± 1.53 IU/L). Significantly (p<0.05) higher serum ALT was observed in groups 2 (50.00 ± 12.52 IU/L) and 3 (56.33 ± 10.21 IU/L). Results for kidney function, shows significantly (p<0.05) higher serum urea concentration in group 3 (13.75 ± 2.41 mg/dl)...
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Posted by admin on Oct 31, 2019 in |
Tamarindus indica seeds – a byproduct of the food industry, are generated in huge amounts during tamarind pulp processing. These seeds are a source of a wide range of polyphenols with natural antioxidant content. In this study, we have encapsulated the polyphenolic components extracted from the seeds in an optimized condition. Statistical optimization of the encapsulation parameters was done using the central composite design of response surface methodology. The concentrations of sodium alginate (m:v, g ml -1), calcium chloride (m:v, g ml-1) and tamarind seed polyphenolic extract (m:v, g ml -1) were the independent variables whose direct and interaction effects were studied on the dependent variable or response of encapsulation efficiency. Further, the encapsulated beads were characterized by physical, chemical, morphological, radical quenching properties as well as textural characteristics. A higher percentage of polyphenols were recovered from beads in simulated gastric fluid, and the rest contents of polyphenols remaining inside the beads were found to be released in simulated intestinal fluid. The study was concluded with the remark that...
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