Posted by admin on Jun 30, 2019 in |
The present study was designed to assess the pharmacokinetics of drug metabolized by hepatic cytochrome 3A4 (CYP3A4) in human volunteers with physical activity at the high altitude hypobaric hypoxic environment. The International Physical Activity Questionnaire short form 7 (IPAQ SF-7) was used to measure the physical activity. The total of 24 healthy male volunteers participated in this study; for them, the intensity of physical activity was measured in terms of metabolic equivalence of tasks (METs). The pharmacokinetic study was conducted using an oral dose of Alprazolam. The blood samples were collected at fixed intervals up to 24 h. The pharmacokinetic variables were found to be changed with increased intensity of physical activity. The metabolism phase was significantly decreased in vigorous and walking physical activity groups. The area under the curve (AUC) and half-life were also decreased. The pharmacokinetics of drug was found to be decreased with increased intensity of vigorous and walking physical activity in humans working at high altitude hypobaric hypoxia environment. It was assumed that oxygenation of...
Read More
Posted by admin on Jun 30, 2019 in |
Adverse drug reactions are considered to be among the leading causes of morbidity and mortality. Approximately 5-25% of hospital admissions are due to ADR’s, and 6-15% of hospitalized patients experience serious adverse drug reactions (SADR’s), causing significant prolongation of hospital stay. The adverse drug reactions reported were classified based on Wills and Brown classification, Causality assessment by the Naranjo’s causality assessment scale, the severity of the adverse drug reactions was assessed using modified Hartwig and Siegel severity assessment Scale. Preventability of the adverse drug reactions was assessed using Schumock and Thornton preventability scale. A total of 108 suspected adverse drug reactions (ADR) were reported during the study period. Highest percentages of ADRs were seen in adults (62.03%) than geriatrics (24.07%) followed by pediatrics (13.89%). The present study concluded that continuous ADR monitoring Programme was found to be useful and beneficial in identifying the percentage of ADR occurrence on an annual basis in the hospital and also helpful to the patient and health care professionals for a better therapeutic outcome....
Read More
Posted by admin on Jun 30, 2019 in |
Saffron is derived from the flower of Crocus sativus as a dried stigma. Kashmiri saffron is the most expensive herb and in sky-scraping demand by herbal and pharmaceutical industries due to which the illegal trade is prevailing in the industry to earn instant Profit. Detection of adulteration and comparison of genuine saffron vs. fake saffron is the crucial aspect which needs a scientific solution. Samples were taken Mogra (A), Lacha (B), Iranian (C) and unknown grade of saffron (D) from Pampore and Anantnag region of Kashmir. The DNA from the samples was extracted with the help NucleoSpin® Plant II Kit and were amplified with the help of reported universal primers of matk, rbcL and MatK, and bidirectional Sanger sequencing was performed. Only ITS gene showed positive extraction and amplification results. ITS gene was capable of distinguishing the interspecific as well as intraspecific variations. The blind trial conducted on unknown grade sample revealed the presence of substituent, which turned out to be Safflower, i.e. Carthamus tinctorius as observed through nucleotide...
Read More
Posted by admin on Jun 30, 2019 in |
Expansion of secondary metabolites from marine resources mainly from echinoderms has been a subject of intense research due to massive bioactive nature. Isolation of new secondary metabolites from sea stars has been found to be highly polar. An initial assessment and validation of these compounds are necessary to assess human risk. Zebrafish are used as a part of a diverse phase of the drug discovery development and has proved to be a constructive and cost-effective alternative to various mammalian models. The current study was performed to observe the effect of sterol isolate from Stellaster equestris on Zebrafish (Danio rerio). The selected active crude extract (which showed enhanced cytotoxic activity) was subjected to purification and was characterized by spectroscopic analysis. The absorption frequency of the FTIR and NMR analysis resembled the absorption frequency of a steroidal compound. The analyzed compound was then evaluated for toxicity using Danio rerio using DNA fragmentation assay, Embryotoxicity, Fin regeneration, and Cardiotoxicity assay as the endpoints. The fishes were exposed to 4 different concentrations (50...
Read More
Posted by admin on Jun 30, 2019 in |
To assess the effect of Perillyl alcohol (POH) on the lipid profile of high-fat-diet-induced hyperlipidemia in white male albino Wistar rats, the hyperlipidemia was induced by feeding cholesterol-rich high-fat diet for 45 days in male albino Wistar rats. The decline in the triglycerides, LDL and VLDL was significant at 100 & 200 mg/kg dose of POH compared to Atorvastatin group. The decrease in total cholesterol was significant at 100 and 200 mg/kg doses compared to hyperlipidemic control. The results demonstrate that POH at a dose of 100 and 200 mg/kg body weight lowers the serum triglyceride, LDL, VLDL, total cholesterol and increase HDL level significantly in high-fat-diet-induced hyperlipidemic rats. Though there was no significant effect on the lipid profile at 50 mg/kg dose. Thus, the results of the present study provide scientific validation for the use of Perillyl alcohol (POH) a terpene as an Antihyperlipidemic...
Read More
