Posted by admin on Apr 30, 2019 in |
Medicinal herbs are a precious heritage for us and since long is a part of traditional as well modern system of medicine. These herbs provide less toxic and more effective drug molecules which are helpful in various ailments. In the present work, we have focused on an edible herb which bears a large number of medicinal properties. Fagopyrum esceulentum Moench (Buckwheat) has a long tradition of being used as food and medicine which is endorsed with abundant of nutrients like flavonoids, polyphenols, proteins, vitamins, phytosterols, carotenoids, and minerals, etc. in it. In the present study, chemical and pharmacological investigation of buckwheat seed ethanol extract were carried out for determining its usefulness in human health. The GC-MS analysis was performed and based on Mass Spectra fragmentation pattern and retention indices, phytoconstituents were identified. Major constituents identified were 9-octadecenamide, n-hexadecanoic acid, ethyl linolate, 9-octadecenoic acid (z), 2, 3-dihydroxypropyl ester, ergost-5-en-3-ol (3.beta.24r), gamma-sitosterol, lupeol, fumaric acid. These phytoconstituents show various pharmacological activities like anti-inflammatory, antioxidant, antibacterial, anticancer, anti-hyperglycemic, dyslipidemic and cardiovascular...
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Posted by admin on Apr 30, 2019 in |
An accurate, precise, sensitive and selective stability indicating High-Performance Thin Layer Chromatographic method has been developed and validated for simultaneous estimation of Amlodipine besylate (AML) and Losartan potassium (LOS) in bulk and pharmaceutical dosage formulation. Chromatographic separation of both the drugs was achieved by using TLC aluminum plates pre-coated with silica gel 60 F254 as the stationary phase and chloroform: methanol: toluene: acetic acid (5.5: 2.5: 2: 0.05 v/v/v/v) as mobile phase. Densitometric analysis was carried out in the absorbance mode at 237 nm. The Rf values found to be 0.23 ± 0.02 and 0.74 ± 0.02 for AML and LOS respectively. The method was validated in compliance with ICH Guideline for linearity, the limit of detection (LOD), the limit of quantification (LOQ), precision, specificity, accuracy, repeatability, and robustness. Linear regression analysis data for the calibration plots showed good linear relationship over the concentration range of 100-400 ng/spot (r2 = 0.9993) and 500-1750 ng/spot (r2 = 0.9991) for AML and LOS respectively. The LOD and LOQ were 24.46 and...
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Posted by admin on Apr 30, 2019 in |
The main objective of this study is to compare the reduction rate of surgical site infection (SSI) between Triclosan coated (TC) and conventional surgical (CS) suture postoperatively. Surgical site infection (SSI) can be presented as a major challenge for people in the sense of mortality and morbidity mostly in developing countries. The result of SSI with antibacterial suture in developed countries have been already reported; however, these interventions have been studied very little in those developing and underdeveloped countries where lifestyle and the hospital facility plays a major role in infection. A single-center randomized controlled trial was conducted at Civil Hospital Aizawl, Mizoram, India. A total of 110 patients were recruited for the study with a 1:1 ratio in both TC and CS. Superficial SSI was assessed as primary outcomes according to the Centre for Disease Control (CDC) guidelines until 30 days. Secondary outcomes also measured for 1st and 3rd days of surgery. Sutures were tested in laboratory condition for their antibacterial activity before use in surgery. Zone of...
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Posted by admin on Apr 30, 2019 in |
Background & Objectives: Standardization of polyherbal Unani formulations is essential to assess the quality of drugs, based on their active principles, physical and chemical standards. This work reports on standardization of a polyherbal Unani powder formulation Safoofe- Musakkin (SM) consist of Rauwolfia serpentina Benth. Ex kurz root, Coriandrum sativum Linn. seed and Piper nigrum Linn. fruit. It is used in neurological disorders. Methods and Material: Plant parts were first cleaned, dried in the shade and powdered by passing through sieve # no. 80 and prepared as per the method described in UPI. SM formulation was evaluated using physicochemical tests: powder characterization, extractive value, alcohol, and water-soluble matter, Ash value, LOD at 105 ºC, pH and HPTLC fingerprinting. Statistical Analysis Used: Mean ± SEM. Result: Organoleptic characters of SM revealed brown color, characteristic odor, bitter taste, and moderately fine texture. Physicochemical parameters resulted in water-soluble extractive (9.58 ± 0.11), alcohol-soluble extractive (6.29 ± 0.07), total ash (5.87 ± 0.09), acid insoluble ash (3.09 ± 0.08), water-soluble ash (1.5 ± 0.05),...
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Posted by admin on Apr 30, 2019 in |
Atherosclerosis results from the slow deposition of fatty materials in media and large arteries leading to mortality worldwide. Terminalia arjuna is an herb of Combretaceae family which contains flavonoids, terpenoids, cardiac glycosides with high antioxidant properties. This study was conducted to determine the effect of hydroalcoholic extract fraction of T. arjuna on blood lipids and atherosclerosis in hyperlipidemia Wistar rats model induced by atherogenic diet. In the hyperlipidemia animal model, the rats received a hydroalcoholic extract of T. arjuna treatment exhibits a noticeable reduction in total cholesterol, total triglycerides and elevation in high-density lipoprotein cholesterol (HDL-C). A result reveals that T. arjuna considerably decreases TC, TG and increases antioxidant levels. Metabolic marker of homocysteine, pro-inflammatory (adipocytokines) markers such as C-reactive protein, leptin, and IL6, anti-inflammatory marker like adiponectin, fibrinolytic factors such as plasminogen activator (PA) and plasminogen activator inhibitor (PAI) that are an association with atherogenic diet consumption. Hence, T. arjuna extract can effectively prevent the progress of atherosclerosis. This is possible due to the effect of T. arjuna...
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