Posted by admin on Jul 31, 2019 in |
Objective: To develop a simple, accurate and precise method for the simultaneous estimation of Moxifloxacin hydrochloride (MOX) and Vinblastine sulfate (VIN). Methods: The normal spectrum of VIN and MOX were converted to its second derivative spectrum and the amplitude minima of VIN and MOX were measured at 214 nm and 297 nm, respectively. MOX and VIN solution were simultaneously determined in 0.1M HCl at 297 nm and 214 nm. Results: The amplitude of MOX and VIN were found to be more distinct in 0.1M HCl with compared to water, methanol and 0.1M NaOH (order = 2 and Δλ=1). Linearity was obtained over the range 1-8 μg/ml and 3-24 μg/ml with a lower limit of quantitation of 1.7 μg/ml and 0.4 μg/ml for MOX and VIN, respectively. For each level of samples, inter- and intra-day precision (% RSD) was <2.1% and <2.3%for MOX and < 2.3 and <2.7 % for VIN, respectively. The mean recovery of MOX and VIN were in the range 99.98%-103.1%and 101.03%-104.3%, respectively. The developed method was...
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Posted by admin on Jul 31, 2019 in |
The present study was an attempt to investigate the effect of extracts and fractions of Mukia maderaspatana on glycemia, lipid profile, lipoprotein level and antioxidant profile in STZ induced diabetic rats for 21 days. Diabetes was induced using streptozotocin (50 mg/kg i.p) and after the induction of diabetes the animals were given with MMEE (100 mg/kg, 200 mg/kg), MMCF (100 mg/kg) and MMBF (100 mg/kg) orally for 21 days. Blood glucose levels were determined by using GOD-POD method with diagnostic kits. The lipid and lipoprotein level was estimated by using the respective kits. The administration of the extracts orally for 21 days showed that there was an amelioration of the lipid and lipoprotein levels significantly. After 21 days the parameters like HDL, LDL, VLDL, TC, TG, Albumin, Creatinine, total protein and glucose were estimated. The treatment with the extracts and fractions of Mukia maderaspatana improved the lipid level and lipoprotein level to a normal condition which may be attributed to its potent antidiabetic activity. The levels of urea and...
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Posted by admin on Jun 30, 2019 in |
In the present investigation, the chalcone intermediates are synthesized by Claisen-Schmidt condensation reaction between acetanilide and appropriate aromatic aldehydes. Further, these chalcone intermediates were cyclized with phenylhydrazine in glacial acetic acid to give new pyrazolines derivatives using ultrasonic irradiation with higher yields in lesser time. All the synthesized derivatives are characterized by their TLC, Physical constant, FTIR, and 1H NMR. The Chalcone intermediates were screened for their antimicrobial activity while cyclized pyrazolines derivatives were screened in search of potential anti-inflammatory compounds. The compound 4c had shown good potential for anti-inflammatory...
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Posted by admin on Jun 30, 2019 in |
The NSAIDs are popular in reducing acute and chronic inflammation as they have no abuse liability. QSAR (Quantitative structure-activity relationship) approach is a very useful and widespread technique for drug design. 2D QSAR models are based on descriptors derived from a two-dimensional graph representation of a molecule. The 2D QSAR study was performed on selected twenty-four compounds from synthesized indole derivatives for elucidating the structural requirements for COX-2 inhibition using multiple linear regression method. Statistically, significant models were generated using VLife Molecular Design Suite 3.5 software. The physicochemical parameters contributed significantly to biological activity. Amongst all the models generated, model 3 was found to be best with high r2 (squared correlation coefficient) of 0.9382. Model is robust as q2 (cross-validated squared correlation coefficient) value is also high as 0.8557 with good predictive power as indicated by pred_r2 = 0.7443. The model showed two alignment independent (AI) descriptors T_2_O_0 and T_2_N_7 as well as two physicochemical descriptors –ve Potential Surface Area and SA Most Hydrophobic contributing for activity. The present...
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Posted by admin on Jun 30, 2019 in |
The entire plant (Monochoria vaginalis (Burm. f.) C. Presl.) except root is being used as a vegetable and has been used in traditional Indian system of medication for treating various diseases. But there are not many scientific data on the antioxidant and anticancer activity of M. vaginalis. Therefore, we aimed to determine the in-vitro antioxidant and anticancer activity of methanolic extract of M. vaginalis leaves. Standard biochemical methods evaluated the phytochemical investigation. In-vitro antioxidant activities of the methanolic extract of leaves were determined against DPPH, hydroxyl radical, reducing power assay, metal chelating activity, and hydrogen peroxide radicals. Also, we evaluated the anticancer activity of methanolic extract on Hep2 and HeLa lines by MTT calorimetric assay. Phytochemical analysis established the presence of rich secondary metabolites of total phenol (52.66 GAE mg/g), and flavonoids (305.88 QE mg/g). The methanolic extract of M. vaginalis exhibited strong antioxidant property (with IC50 value) against DPPH (52.24 mg/ml), hydroxyl radical (76.08 mg/ml), reducing power (58.15 mg/ml), metal chelating activity (35.70 mg/ml), and hydrogen peroxide scavenging...
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