Posted by admin on Mar 31, 2019 in |
In this paper Chromium (VI) based Pyridinium fluoro chromate (PFC) reagent has been used as an oxidant for the determination of some antihistamine drugs e.g. Promethazine hydrochloride (PMH) and Pheniramine maleate (PM) in pure form and their pharmaceutical preparations such as Phenergan (Injection and tablet) and Avil (Tablet and injection) respectively. The main principle of this method is based on the fact, that each pharmaceutical drug contains a specific organic functional group, which on oxidation in the presence of selected oxidant (Here PFC is used as an oxidant) provides the new oxidized product. This type of oxidation reaction between the drug molecule and an oxidant establishes a stoichiometric relationship between the drug molecule and an oxidant. This relationship is the basis of quantitative estimation of drugs in pure form and their pharmaceutical preparations. In this research, oxidizing reagent Pyridinium fluoro chromate (PFC) oxidizes the sulfur atom of Promethazine hydrochloride (PMH) to the corresponding sulphoxide, whereas in case of Pheniramine maleate (PM) it oxides one hydroxyl (-OH) group of carboxylic...
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Posted by admin on Mar 31, 2019 in |
It is well known that Trichoderma sp. can be used as a biological control agent (BCA). In this study, 02 new Trichoderma isolates were obtained from 35 different soil samples of Punjab, and Himachal Pradesh and its bio-control/antifungal effects were determined against 07 newly isolated postharvest fungal pathogens (PHFP) from 35 different samples of fruits and vegetables collected from storage and selling markets of Chandigarh. In-vitro evaluation of antagonistic activity of only one newly isolated bio-control agent Trichoderma viride (RS1) was done by dual culture method (DCM) and culture filtrate technique (CFT) and compared with the standard BCA, T. viride MTCC 800. Results of CFT were significantly higher than by DCM in case of newly isolated BCA in comparison to standard BCA. Further, a pot experiment was carried out to check the effect of fungal pathogens and bio-control agent on the growth of barley seeds. It was observed that in the presence of BCA alone as well as in combination with PHFP, the negative effect of fungal pathogens reduced...
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Posted by admin on Mar 31, 2019 in |
Simple, rapid validated stability-indicating HPLC method for estimation of Salmeterol xinafoate was successfully developed. The separation was achieved by using a mobile phase of buffer: methanol in the ratio of 60:40 v/v using HiQ SiL C18 column (150 × 4.6 mm i.d. 5 μm) at 1.2 mL/min as flow rate. The detection was carried out at 252 nm using a PDA detector. The retention time observed was 11.89 ± 0.3 min. This drug was subjected to stress conditions as per ICH Q1A (R2). Linearity was found to be in the concentration range of 10-50 μg/mL with r2 = 0.9958. The suitability of this HPLC method for quantitative estimation of Salmeterol xinafoate was proved by validation by the requirements of ICH guidelines Q2A...
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Posted by admin on Mar 31, 2019 in |
Vitex agnus-castus L. (Verbenaceae) commonly known as Chaste tree, is a shrub widely distributed in the Middle East and Southern Europe. Traditionally used for the treatment of several health problems and symptoms, such as premenstrual ones and spasmodic dysmenorrhea, certain menopausal conditions, insufficient lactation, and acne. Several reports have indicated that Vitex agnus-castus contained flavonoids, diterpenoids, and essential oils. To our knowledge, no data are available on the antioxidant activities of Moroccan Vitex agnus-castus. This work aims to evaluate the antioxidant activity of four extracts from leaves of Vitex agnus-castus growing wild in Morocco. Phytochemical tests of the extracts (ethanol, methanol, ethyl acetate, and water) were carried out qualitatively for the presence of Alkaloids, Tannins, Anthraquinones, Terpenes, and saponins. In-vitro antioxidant activity, total phenols content and total flavonoid content of different extracts were determined using spectrophotometric methods. The total phenolic content ranged from (10 ± 0.2 to 53.33 ± 1.38 mgEq GAE/g DW), and the total flavonoid concentrations varied from (13.66 ± 0.33 to 95.33 mg RE/gDW). Ethanolic extract of...
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Posted by admin on Mar 31, 2019 in |
A new simple, rapid, and sensitive reversed-phase liquid chromatographic method was developed for the estimation of doxofylline and terbutaline in the tablet dosage form. The chromatographic separation was achieved on ODS C18column (150 × 4.6 mm, 5 µm) at ambient temperature and effluent monitored at 257 nm. The mobile phase consists of ammonium acetate buffer (pH adjusted to 3 with o-phosphoric acid) and acetonitrile in the ratio of 50:50 v/v. The flow rate was maintained at 1 ml/min. The method was validated concerning linearity, precision, accuracy, ruggedness, limit of detection, limit of quantification and robustness. The assay methods were found to be linear from 16-96 µg/ml for doxofylline and 0.2-1.2 µg/ml for terbutaline. All validation parameters were within the acceptable range. The mean recovery was 99.35 and 99.25 for doxofylline and terbutaline respectively. The % RSD value was found to be less than 2. The limit of detection and limit of quantification for doxofylline and terbutaline were found to be 0.06 μg/ml and 0.024 μg/ml and 0.21μg/ml and 0.079...
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