Posted by admin on May 31, 2019 in |
Helicobacter pylori (H. pylori) is a spiral, gram-negative pathogen which causes gastric cancer along with MALT lymphoma. It is widespread among the gut microflora and stands first among the pathogens. Cytotoxin-associated gene (cagA) is thought to be the vital protein in the pathogenesis of gastric cancer. Since there are no available vaccines at the moment, and owing to the increasing antibiotic resistance, studies towards developing a novel vaccine are very much essential. The present study was designed to clone and express the protein cagA within the bacterial systems. The protein fragment was screened for antigenic properties using in-silico studies. The protein of interest was confirmed using the western blots. This study further can be planned to produce a novel peptide...
Read More
Posted by admin on May 31, 2019 in |
Present study highlights that plant as a source of medicine has been inherited and is an important component of the health care system in India. Ziziphus mauritiana fruit (Rhamnaceae), one of the medicinally important plants commonly found in subtropical countries, was chromatographically evaluated in the present work for the identification of various phytochemical compounds found in it. The phytochemical tests showed the presence of alkaloids, flavonoids, phenols, saponins, tannins in methanolic extract of Ziziphus mauritiana (MEZM). Majorly 24 compounds in 93.08% peak area were identified through spectrum matching with National Institute Standard and Technology (NIST) database. In accordance with known therapeutic effects of the identified compounds like 5-Hydroxymethyl furfural; 1, 5-Anhydroglucitrol; Polygalitol; Sedoheptulosan; D-Allose; Beta-D-Glucopyranoside Methyl; 3, 4-Altrosan; Nonanoic acid; Octanoic acid; 2-Hexyl- 2-Hexadecanoic acid; 2-Propyloctanoic acid; Molinate; Levetriracetam; Clindamycin and Maltol, the MEZM fruit’s justified a very good source of therapeutic agents for Cancer, Epilepsy, Alzheimer’s disease, Parkinson disease, Amyotrophic Lateral Sclerosis (ALS), bacterial and fungal infections. Also, it is rich in compounds comprising anti-oxidant, anti-spermatogenic, anti-biotic, neuroprotective...
Read More
Posted by admin on May 31, 2019 in |
Artocarpus heterophyllus is an evergreen tree cultivated and consumed throughout Nepal; however, its seed has been neglected as compared to the fruit. The sample was screened for phytochemical evaluation, and general tests, DPPH activity, antibacterial activity, and total flavonoid content was also measured. Also, the potential of its use as functional food was highlighted. Phytochemical analysis revealed the presence of flavonoid in both seed and peeled whereas alkaloid and saponin were detected in peel only. The general tests revealed that acid value (8.52), saponification value (14.02), ester value (5.49) as well as moisture content (12.37%) were low. Further, DPPH scavenging assay shared that the peel (IC50 70.97 µg/ml) has better antioxidant activity than seed (IC50 199.12 µg/ml) which could be correlated to total flavonoid content (132.05 ± 19.46 mg QE/g dry extract of peel and 67.38 ± 11.51 mg QE/g dry extract of seed). Anti-bacterial activity of seed was detected against Proteus mirabilis and Salmonella entericatyphi only while that of peel was not detected. Various researches support the fact...
Read More
Posted by admin on May 31, 2019 in |
Objective: The prodigious challenge in the pharmaceutical industries was to enhance the solubility and the permeability of those drugs as key factors to improve their bioavailability. Various techniques have been used to improve the drug water solubility and release profile, and solid dispersions are considered to be the most successful techniques. The aim of the present study was to improve the solubility and bioavailability of a poorly water-soluble antihypertensive drug (Eprosartan) in the human body, using a solid dispersion technique (hot melt extrusion) and comparison with other methods. Methods: The development of solid dispersions as a practically viable method to enhance the bioavailability of poorly water-soluble drugs to overcome the limitations of previous approaches such as salt formation, solubilization by co-solvents and particle size reduction studies revealed that drugs in solid dispersion need not necessarily exist in the micronized state. Solid solution was prepared by solvent evaporation, fusion method, hot melt extrusion technique at 1:1.1, 1:2 (Eprosartan: Soluplus) and 1:1.5:0.5 (Eprosartan:Soluplus: Kollidan/Plasdone) ratios respectively. Solid Solution was evaluated for...
Read More
Posted by admin on May 31, 2019 in |
The purpose of the present study was to formulate Losartan potassium compression-coated tablets using okra gum as a binder along with synthetic hydrophilic polymers like various grades of Hydroxypropyl methylcellulose (HPMC) and compare the different parameters. The effect of different concentration of okra is to be used in the formulations with varying concentration of the polymers, elucidating the effect of type of polymers and its concentration on release pattern of the drug from the compression-coated tablets. Okra gum was extracted by using acetone. After subsequent evaluation of the gum and the preformulation studies of the drug, fast disintegrating core tablets were prepared. Okra gum was used as binder in varied concentrations (3%, 4% & 5%) and three different grades of Hydroxypropyl methylcellulose (HPMC) were used in 30%, 60% & 90% concentrations to prepare compression coated tablets. Characterization was done, and the drug release pattern was compared among the batches. Preformulation studies like differential scanning calorimeter (DSC), Fourier transform infrared spectroscopy (FTIR) showed that there was no significant interaction between the...
Read More
