Posted by admin on Apr 30, 2019 in |
This study aimed to investigate the acute toxicity of methanolic extract of Chrysophyllum perpulchrum on biochemical, behavioral and histopathological variation in male Wistar rats. Acute toxicity of the methanol extract of Chrysophyllum perpulchrum was carried out on 36 male rats using different doses of the extract ranging from 500 to 2000 mg/kg body weight. Behavioral tests were used to determine the effect of the extract on cognitive performance such as anxiety, memory, and locomotor activity. Biochemical analyses of (ALAT, ASAT, glycemia) were carried out on the plasma, while pathological changes in the kidneys, liver were examined histologically. Our results showed that the LD50 value indicates that the methanolic extract of the C. perpulchrum administered orally, is slightly toxic and can induce some slight behavioral disturbances related to anxiety whereas the learning and memory capacities remained unaffected. The acute oral treatment of rats with methanolic extract of Chrysophyllum perpulchrum, in general, did not induce significant modifications of the biochemical profile when compared to the control group. However, the histopathological evaluation...
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Posted by admin on Apr 30, 2019 in |
The acidic pH of the digestive vacuole (DV) of P. falciparum is thought to play a major role in the mechanisms of digestion of host erythrocyte hemoglobin, detoxification of heme, and chloroquine (CQ) action and resistance. However, a definitive measurement of the DV pH has been technically difficult. A previous study by Abu Bakar (2015) measured the DV pH by using fluorescein isothiocyanate-dextran (FITC-dextran), a ratiometric pH indicator loaded into the malaria parasite’s DV that had been isolated using saponin (DVsap). To validate the FITC-dextran response to the DVsap pH, pH of the DV isolated by digitonin permeabilization (DVdigi) was measured. The DVdigi pH of the CQ-sensitive parasite (D10) was 5.66 ± 0.07 that is approximately similar to that of the DVsap pH (5.27 ± 0.03) estimated using the same probe. The DVdigi pH of the CQ-resistant parasite (Dd2) (5.62 ± 0.12) was not significantly different from the CQ-sensitive parasite’s DVdigi pH (P> 0.3). Re-acidification of the DVdigi was also observed upon the addition of 2 mM ATP to...
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Posted by admin on Apr 30, 2019 in |
The assessment of the probability of occurrence of deep vein thrombosis played a very important part in making a correct diagnosis and modified Wells’ score is a widely used prediction rule for pre-test probability assessment. We aimed to evaluate the diagnostic accuracy of the modified Wells’ score in the diagnosis of lower extremity deep vein thrombosis compared to Doppler ultrasound study. This is a cross-sectional study conducted in Baghdad Teaching Hospital from Jan 2017 till Dec 2017. Adults inpatients suspected of having lower-extremity deep vein thrombosis were included after taking verbal consents. Patients on antithrombotic treatment and those suspected to have pulmonary embolism were excluded. According to Wells’ score; patients were divided into likely and unlikely groups. Lower extremity venous duplex ultrasound study was established. Correlation of Wells’ score with DVT and its efficacy was analyzed using receiver operating characteristic curve. A total number of 113 patients were included; with a mean age of 40.6 ±, 12.7 years and males were 60 (53.1%). Based upon Wells’ score, 45 (39.8%)...
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Posted by admin on Apr 30, 2019 in |
A series of new quinoline/chalcone hybrids 3a-e and 4a-e were designed and synthesized. The structures of all new target molecules 3a-e and 4a-e have been confirmed by various spectral techniques and elemental analyses. The newly synthesized compounds were screened for their antibacterial activity using agar disc diffusion method. Results showed that most of the newly synthesized compounds showed good antibacterial activity comparable with that of the standard drug Gatifloxacin against all tested strains (B. cereus, E. coli, and S. marcescens) except for P. aeroginosa where it does not respond to most of the newly synthesized compounds, and that compounds 3c, 4c, 4d, and 4e showed good antibacterial activity (53-78% that of Gatifloxacin) towards some bacterial strains (Bacillus cereus, Escherichia coli, and Serratia marcescens) when compared to standard drug Gatifloxacin. Amongst all the tested compounds, 4e exhibited excellent activity against S. marcescens (88% that of...
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Posted by admin on Apr 30, 2019 in |
The objective of the study was to formulate coated liposomes containing Prednisolone for colon targeting and localization of the therapy to the inflamed colon mucosa. Liposomes were prepared using thin film hydration method. Liposomal formulations were optimized using 32 factorial design using varying concentrations of SPC and cholesterol as independent variables and % EE as the dependent variable. Optimized formulation F5 (60:30) was selected for successive coating with chitosan and eudragit S-100. Chitosan concentration of 1% w/v was taken as optimal based on ZP values. The coated liposomes were encapsulated in eudragit S-100 shell using a solvent-free method. The size if liposomes increased upon addition of the coatings. Fluorescent microscopy aided in clear visualization of chitosan coating around the vesicles. SEM images showed a transition surface appearance in the order smooth-rough-smooth for F5 and the coated liposomes. Eudragit S-100 coated liposomes were subjected to in-vitro drug release studies using simulated fluids for 16 h. ECL5 with 2% w/v eudragit showed sufficient lag time of 6 h. The formulation was...
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