Posted by admin on Mar 31, 2019 in |
The low bioavailability of drugs in the oral drug delivery system is the main concern and the development of a delivery system with a controlled release profile. This research aims in the dose reduction of aliskiren hemifumarate, evaluate the release efficacy of the drug from the patches by using various permeation enhancers. FTIR studies established the drug and polymer compatibility. The transdermal patches were prepared by using a solvent casting method using different polymers and permeation enhancers. The keshary chein diffusion cell was used for performing in-vitro permeation studies. By physicochemical parameters and in-vitro release pattern, the formulation made from MethocelK 15M (1:1.5) was selected for in-vivo studies. The steady plasma concentration was observed on application of developed transdermal patches, as confirmed by bioavailability studies in rats. The bioavailability was also improved as compared to oral administration. The SEM studies of transdermal patches show that the patches prepared with MethocelK 15M with cineol as penetration enhancer exhibited the uniform distribution of the drug. The hypertension was induced in rats...
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Posted by admin on Mar 31, 2019 in |
Centella asiatica L. Urban (family: Umbelliferae) contains several active triterpenoids which have been reported to induce type I collagen which decreases substantially in photoaged skin. Centella asiatica extract is highly soluble in water but have low absorption into the skin. To overcome this, water in oil nanoemulsion loaded with Centella asiatica extract have been formulated, optimized and evaluated as the potential delivery system for treating photoaging. Nanoemulsions were formulated by the oil phase titration method and optimized by constructing pseudo-ternary phase diagrams. The prepared nanoemulsions were subjected to thermodynamic stability testing. Those that passed these tests were characterized for droplet size, polydispersity index, zeta potential and droplet morphology by Cryo-SEM. The prepared nanoemulsions were in nanometric range (optimized batch: 140.5 nm) with uniform size distribution (PDI: 0.086) and good physical stability (zeta potential: -33.9 mV). Topical permeation of Centella asiatica loaded nanoemulsion through porcine abdominal skin was estimated using the Franz diffusion cell. A significant increase in permeability parameters was observed in nanoemulsion formulations (P<0.05) as compared to an...
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Posted by admin on Mar 31, 2019 in |
Introduction: Oral carcinoma is amongst the leading malignancies worldwide, with squamous cell carcinoma (SCC) as the most common type accounting for over 90% of cases. In OSCC, MMPs, (MMP9) which belongs to gelatinase group, promote tumor progression by new blood vessel formation, disturbing tissue morphology which allows tumor growth breaks the basement membrane and enables metastasis and it’s overexpression in OSCC have prognostic value. Aims and Objectives: To evaluate the expression of MMP 9 in different grades of OSCC. Material and Methods: In this prospective study, a total number of 30 blocks specimens; 10 of each well, moderately, poorly differentiated were stained with MMP9 antibodies and compared with normal oral mucosa. All the data were documented and analyzed with SPSS 20 version software. Results: Normal 10 tissues with a mean age of 31.30 years and 30 OSCC patients of 51.93 years mean age were studied. In OSCC, 18 male (60%) and 12 female (40%) were participated. Among them, 8% smoking, 15% chewing and 7% both habits were revealed. With...
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Posted by admin on Mar 31, 2019 in |
Neurodegeneration accounts for diseases like Parkinson’s disease, Alzheimer’s disease, Huntington’s disease, etc. that come under neurodegenerative diseases. Parkinson’s disease is the second most common neurodegenerative disease. The main hallmarks of Parkinson’s disease are progressive loss of motor control and cognitive dysfunction. Current treatments are of reducing the symptoms, do not address the long term complications like motor and cognitive dysfunction and have serious side effects as well. Recent research has linked tyrosine kinase activity dysfunction and mitochondrial dysfunctions to the main etiologies of Parkinson’s disease of familial and sporadic origin. Since Parkinson’s disease occur due to multiple etiologies, using a combination of drugs that target different etiologies could be an ideal strategy to address neurodegeneration. In the present study, the neuroprotective efficacy of a combination of tyrosine kinase inhibitor; dasatinib and natural antioxidant resveratrol were studied. Stereotaxic infusion of rotenone was given to rats to induce Parkinson’s disease, with nine treatment groups. The neuroprotective efficacy of the drug combinations was evaluated in different treatment groups by checking, behavioral studies,...
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Posted by admin on Mar 31, 2019 in |
Objective: The objective of this research was a synthesis of silver nanoparticles from Murraya koenigii leaves in which the particles were characterized by various techniques and to evaluate the potential antibacterial activity of synthesized nanoparticles against various human pathogens. Methods: Murraya koenigii aqueous extract of leaves were prepared by the Soxhlet method, and the bioactive compounds were analyzed by phytochemical analysis. Silver nanoparticles were synthesized which are characterized by UV-visible spectroscopic analysis, XRD, TEM, FTIR analysis and antimicrobial activity was evaluated by agar well diffusion method. Results: Phytochemical analysis of Murraya koenigii aqueous extract of leaves showed the presence of alkaloids, phenols, flavonoids, terpenoids, coumarins, and steroids. The aqueous AgNo3 solution changed from green to dark brown color after incubation. UV-visible spectroscopic analysis showed the absorbance peak at around 420 nm which indicates the synthesis of Ag NPs. TEM image showed the formation of well-dispersed silver nanoparticles in the range of 5-25 nm. X-ray diffraction (XRD)-spectrum as evidenced by the peaks at 2θ values of such peaks 38.43°, 44.40°, and...
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