Posted by admin on Jan 31, 2019 in |
Plants have been studied since ages for their therapeutic uses. In the earlier time, not much was known about the phytocompounds, but today the constituents of the different plants have been extracted and isolated for the use in the name of medicines or drugs. Flavonoid is one of the most characteristic classes’ of compounds in higher plants known to play several important roles including therapeutic uses in cancer treatment. The activation of oncogenes in the cancer cells can be regulated by the selective tyrosine kinases inhibitors, and therefore it can be considered as a promising approach for the targeted therapeutic development. Hence, in the present study, the authors tried to combine the knowledge of using plant Launaea procumbens in traditional medicine by analyzing and identifying the specific phytocompounds, especially flavonoids, and evaluating its anticancer potential. The scope of the present study is based on the aim to determine the possible use of plant Launaea procumbens in the field of therapeutics for cancer studies by performing in-silico docking analysis of...
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Posted by admin on Jan 31, 2019 in |
Fluoride becomes toxic at higher doses which leads to Fluorosis. In addition to dental and skeletal fluorosis, it also affects soft tissues including liver, heart, kidney, muscle, brain, etc. The aim of this study to examine the fluoride-induced oxidative stress and the protective role of Allium sativum ethanolic extract (ASEE) against underlying fluoride induced neurotoxic effects in the brain of rats. Three months old (250-280 wt.) Wistar rats were randomly categorized into 4 groups which were control (received normal tap water), sodium fluoride (NaF) (20 ppm fluoride through IP), NaF (20 ppm) + ASEE (120mg/kg body wt; through oral with plastic gavage), and ASEE. The doses are continued for 15 days, and on completion of treatments behavioral (Rotarod and Hot plate test), oxidative stress markers (LPO, SOD, CAT, GSH-Px) and Histological (Golgi Cox staining) observations were made. NaF treated rats showed significantly (p<0.01) decreased motor coordination, thermal pain response, SOD and Catalase activity. Whereas, LPO levels and GSH-Px activity increased in NaF treated rats. Moreover, they also showed a...
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Posted by admin on Jan 31, 2019 in |
Objective: To develop accurate, precise stability indicating a method for simultaneous estimation of Elbasvir and Grazoprevir in bulk and pharmaceutical dosage form. Materials and Methods: Simple, rapid, precise, sensitive and reproducible validated stability-indicating Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) method for the quantitative analysis of Elbasvir and Grazoprevir in the pharmaceutical dosage form. Chromatographic separation was carried out on waters Alliance-2695, by using Luna C18 (150 mm × 4.6 mm, 5 µm) column and the mobile phase containing OPA buffer (0.1%) and acetonitrile in the ratio of 50:50 v/v. The flow rate was 1.0 ml/min; detection was carried out at 258 nm using a photodiode array detector at ambient temperature. Results: The number of theoretical plates and tailing factor for Elbasvir and Grazoprevir were obtained to be NLT 2000 and should not more than 2 respectively. The linearity of the method was excellent over the concentration range 1.53-22.95 µg/ml and 3.05-45.75 µg/ml for Elbasvir and Grazoprevir respectively. The correlation coefficient was 0.999%. The relative standard deviation of peak areas of...
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Posted by admin on Jan 31, 2019 in |
Introduction: Autism spectrum disorders (ASD) are a group of mental problems that interfere with social interaction skills, and behavioral spectrum. ASDs are one of the most prevalent developmental disorders among children. The purpose of this study was to examine the mental health, quality of life, and happiness in mothers who had ASDs children and compare them with mothers having healthy children. Materials and Methods: This is a descriptive-analytic study. The population consisted of all mothers of children with autistic spectrum disorders (ASD) in Guilan province. The samples were determined through convenience sampling. We included 90 mothers whose children were identified with autism according to DSM5 criteria based on the clinical diagnosis of the child and adolescent psychiatrist and 90 mothers of children with no psychological complaint. The two groups were matched regarding demographic characteristics. The mothers of both groups completed a demographic questionnaire, the Symptom Checklist-90-R (SCL-90-R), the SF-36 Quality of Life Questionnaire, and the Oxford Happiness Questionnaire. The results were analyzed using descriptive statistics (i.e., mean, standard deviation)...
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Posted by admin on Jan 31, 2019 in |
Standardization is a process involving various chemical analysis methods based on pharmacological data involving general physical and microbiological analyzes aimed at the safety of a natural extract. To obtain the correct result, the standardization step is preceded by method validation. Quercetin can be obtained from the leaves of soursop (Annona muricata Linn.), which use for treatment fever, diarrhea, anti-convulsive, antifungal, antimicrobial, gout, itching, and flu. Determination of quercetin in soursop leaf extract using TLC-Densitometry must be validated by testing selectivity, limit of detection (LOD), limit of quantification (LOQ), linearity, precision, accuracy, and range. Densitometry scanning of the plates silica gel 60 F254 directly at 265 nm was used for analysis of quercetin. The result of selectivity test was 1.53 (>1.5) with mobile phase toluene: ethyl acetate: formic acid (4: 3: 0.4; v/v/v), limit of detection (LOD = 0.018 µg), limit of quantification (LOQ = 0.060 µg), linearity y = 9387.3x – 767.39 (r = 0.9996 and Vxo = 1.9%), Coefficient of Variation (C.V.) of precision 1.906525% and the percent...
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