Posted by admin on Feb 28, 2019 in |
The objective of the present study was to develop mouth dissolving films (MDF) of Losartan potassium for the treatment of hypertension, with fast disintegration, optimum morphological properties, and mechanical strength. Losartan is an anti-hypertensive drug which undergoes extensive first-pass metabolism that results in low bioavailability of the drug. Through buccal route, the drug directly enters blood circulation and hence bioavailability of the drug increases. Hydroxypropylmethyl-cellulose, sodium carboxymethylcellulose, sodium alginate, and gelatin were used as the hydrophilic film-forming polymeric bases and glycerol as plasticizer. Films were prepared by solvent casting technique. Parameters like in-vitro disintegration time, tensile strength, content uniformity, folding endurance, swelling index, and in-vitro drug release were evaluated. 32 factorial design was used to optimize the amounts of the polymer and the plasticizer. In-vitro dissolution studies showed that 99% of Losartan potassium was released within 5 min with an average disintegration time of 38 sec. UV and FTIR spectrophotometry were used to identify drug-excipient interactions. Accelerated stability studies were performed as per ICH guidelines wherein the MDFs were...
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Posted by admin on Feb 28, 2019 in |
Uterine fibroids are the most common genital tract tumor in reproductive age women. The main treatment option for fibroid is surgical intervention. Options for medical therapy are currently limited to preoperative reduction of symptoms related to uterine bleeding and fibroid size. Ayurveda treatment for this condition is widely practiced in Sri Lanka. We aimed to evaluate the efficacy of two Ayurveda treatment regimes in patients with uterine fibroids. The study is designed as a randomized, single blind, three arm clinical study with 30 participants in each arm. Participants diagnosed with uterine fibroids size 2 cm or more and meet the inclusion criteria are enrolled to this study. Arm I and Arm II undergo two Ayurveda treatment regimens and Arm III is the control group. Endpoint will be calculated from baseline in volume of the largest fibroid by two dimension ultrasound scan after 12 weeks of treatment intervention and repeat in four weeks follow up. Clinical improvements and quality of life is assessed by Uterine Fibroid Symptom and Health-Related Quality...
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Posted by admin on Feb 28, 2019 in |
In the present study, comprehensive stress testing of Carfilzomib, a newly approved proteasome-inhibiting anticancer drug was carried out according to ICH guideline Q1A (R2). The drug was subjected to acid (0.1N HCl), neutral and alkaline (0.1N NaOH) hydrolytic conditions at 70 ºC, as well as to oxidative decomposition at room temperature. Photolysis was carried out in 0.1N HCl, water and 0.1N NaOH at 40 ºC. LC-PDA and LC-MS investigated the products formed under different stress conditions. The LC-PDA method that could separate all degradation products formed under various stress conditions involved a C18 column and a mobile phase comprising of ACN and phosphate buffer (pH 3). The flow rate and detection wavelengths were 1 ml/min and 220 nm, respectively. The developed method was found to be precise, accurate, specific and selective. It was suitably modified for LC-MS studies by replacing phosphate buffer with water, where pH was adjusted to 3.0 with formic acid. The drug showed instability in the solution state (under acidic, neutral, alkaline and oxidative stress conditions),...
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Posted by admin on Feb 28, 2019 in |
A series of conjugation of two heterocycles 1, 3, 4-oxadiazole and quinazolone were synthesized and screened for antimicrobial as well as antioxidant activity. Compound4-(4-oxo-2-phenyl-quinazoline-3(4H)-yl)-benzohydrazide 3 on cyclization with different aromatic acids in the presence of phosphoryl chloride (POCl3) give different 1, 3, 4-oxadiazole derivatives. Similarly, compound 3 on reaction with carbon disulphide (CS2) gives substituted oxadiazole thione which on reaction with different halides in the presence of dimethylformamide (DMF) give oxadiazole thiones analogs 6a-6c. Structural assignments of these compounds have been made by elemental analysis, UV, IR, 1H NMR, and mass spectral data. Synthesized analogs were screened for in-vitro growth inhibition activity against different strains of bacteria and fungi and compared with standard drugs ciprofloxacin and fluconazole. Compounds 4e and 4f have good activity against bacteria. All compounds have moderate activity against fungi. These compounds were screened for antioxidant activity by using radical scavenging DPPH assay by using ascorbic acid as a standard drug. Compounds 4e, 6a to 6c have good antioxidant...
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Posted by admin on Feb 28, 2019 in |
Samanea saman is a tropical avenue tree which is widely cultivated in Northern America, and Asia belongs to the family Leguminosae. It possesses a wide range of biological perspectives. Folklore claims postulated that Samanea saman is having an antiulcer, antioxidant antibacterial and anti-mycobacterial properties. In the present investigation, the methanol extract of leaves of Samanea saman has been prepared based on the polarity and subjected to column chromatography. Based on this principle, the bioactive alcoholic extract (5%) was fractioned over a Silica gel (100-200 mesh) column by eluting with solvents of increasing polarity in the order of benzene: ethyl acetate and methanol. Similar TLC fractions were being combined and characterized by spectral analysis. Spectral data are revealed the presence of phytocompounds from the methanolic fractions of the Samanea saman. It was found that the compound named Pithecolobine showed remarkable activity against Mycobacterium tuberculosis by screening through Alamar blue assay (MIC at 3.12 µg/ml) and Luciferase assay (86% inhibition at 10 µg/ml) apart from other compounds obtained. The compound was...
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