Posted by admin on Mar 31, 2019 in |
A new simple, rapid, and sensitive reversed-phase liquid chromatographic method was developed for the estimation of doxofylline and terbutaline in the tablet dosage form. The chromatographic separation was achieved on ODS C18column (150 × 4.6 mm, 5 µm) at ambient temperature and effluent monitored at 257 nm. The mobile phase consists of ammonium acetate buffer (pH adjusted to 3 with o-phosphoric acid) and acetonitrile in the ratio of 50:50 v/v. The flow rate was maintained at 1 ml/min. The method was validated concerning linearity, precision, accuracy, ruggedness, limit of detection, limit of quantification and robustness. The assay methods were found to be linear from 16-96 µg/ml for doxofylline and 0.2-1.2 µg/ml for terbutaline. All validation parameters were within the acceptable range. The mean recovery was 99.35 and 99.25 for doxofylline and terbutaline respectively. The % RSD value was found to be less than 2. The limit of detection and limit of quantification for doxofylline and terbutaline were found to be 0.06 μg/ml and 0.024 μg/ml and 0.21μg/ml and 0.079...
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Posted by admin on Mar 31, 2019 in |
Tuberculosis (TB) is an alluring affliction caused predominantly by Mycobacterium tuberculosis bacteria. A stumbling block in the conventional treatment of TB is the occurrence of multiple drug resistance (MDR) followed by high dose requirement, subsequent intolerance & severe toxicity. Nanoparticulate-based drug therapy has significant potency in the treatment of TB. In this present study, Linezolid loaded Eudragit RS 100 polymeric nanoparticles (LZD-PNPs) were developed and characterised for the treatment of TB. Double emulsion solvent evaporation method was selected to incorporate LZD into Eudragit RS 100. Differential scanning calorimetry (DSC), Fourier Transfer Infrared spectroscopy (FT-IR) studies were done to assess drug-polymer compatibility and to indicate absence of incompatibility. Particle size analysis was carried out to measure the mean particle size of the LZD-PNPs and was found to be at 47nm-119nm (±26 to 41 SD) which indicated the polydispersity of the formulation. Zeta potential analysis of the sample was found to be -32 to -41mv, indicating its physicochemical stability. The Entrapment efficiency of LZD-PNPs ranged between 75.56% to 80.42%. Optimization of...
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Posted by admin on Mar 31, 2019 in |
Background and Objectives: It is well realized that cervical cancer is one of the most critical diseases in the world. This study has been laid down for molecular detection (qualitatively and quantitatively) and genotyping of high and intermediate risk HPV genotypes in patients with high suspicion of cervical cancer. Patients and Methods: A total of 120 females were included in this study. DNA has been extracted automatically using Samaga DNA extraction unit. Real time-PCR technique for detection and genotyping of high and intermediate risk genotypes was performed. Results: The genes amplification revealed that only 9(11.3%) out of 80 were positive for high-risk HPV. The genotyping study yielded that the most prevalent genotypes were HPV 16, 18, 39, 33 and 35. Among the result of high-risk genotypes of HPV, the viral load was 6.42 ± 0.71 for clinical status CIN III. Only 11 out of 20(55 %) of FFPE were positive for high-risk HPV. On the other hand, The clinical status of high-risk HPV genotypes from FFPE samples were 4(20%),...
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Posted by admin on Mar 31, 2019 in |
Motion sickness (MS) is a conflict between visual and vestibular system leading to nausea and vomiting. The present study aimed to standardize a method to induce MS in Balb/c mice model. Conditioned taste aversion (CTA) utilizing saccharin solution (SS) was used as MS index. Mice were divided into three groups (n=6); group 1 (G1), group 2 (G2) and group 3 (G3). Two bottle test [TBT, both bottles consists of either drinking water (DW) or SS] and two bottle choice test (TBCT, one bottle comprises of DW and other SS) were performed. G2 and G3 experienced rotation for 15, 30 and 45 min using a dancing shaker whereas G1 received no rotation. The consumption of SS before and after MS induction was recorded. Acetylcholine esterase and cortisol levels were detected in mice brain, and HPLC-ECD detected plasma samples and neurotransmitters. It was found that the rotation of 30 min resulted in a significant reduction in the SS consumption in the G3 group when compared to G1, thus indicating that the...
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Posted by admin on Mar 31, 2019 in |
Recent advances in novel drug delivery aims to enhance the safety and efficacy of drug molecule by formulating a convenient dosage form for ease of administration and to achieve better patient compliance. One such approach is oral disintegrating tablets. Oro-dispersible tablets are a suitable means of drug delivery system for better patient compliance, rapid onset of action, increased bioavailability. The purpose of the present research was to formulate and evaluate the mouth disintegrating tablets of metoclopramide hydrochloride. Metoclopramide hydrochloride was used as the active drug, and superdisintegrants like sodium starch glycolate (SSG), crospovidone (CP) and croscarmellose sodium (CCS) were used in the formulation. A total of 7 formulations were fabricated using a direct compression method. The amount of superdisintegrants was varied in each formulation. All the formulations were subjected to pre and post compression parameters. Preformualtion stability study was done to ensure the drug-excipient compatibility study. The effect of superdisintegrants on wetting time, disintegration time and dissolution profile were evaluated. Among the formulated batches, the formulation containing CP 5%...
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