Posted by admin on Nov 30, 2018 in |
The whole plant extracts of N. crociformis and N. infundibulifolia was analyzed by priliminary phytochemical, total phenolics, flavonoid, carbohydrate and alkaloid content by standard methods. In-vitro antioxidant DPPH assay and Phosphomolybdenum assay developed by Braca et al., Prieto et al., respectively. In-vitro antibacterial (agar well diffusion) activity by gram negative Pseudomonas aeroginosa (ATCC 27853), gram positive (Streptococcus mutans MTCC 890 & Staphylococcus aureus ATCC 25923, Esteritia coli ATCC 25922) and antifungal activity by Aspergillus niger (ATCC 16404). The study shows that the petroleum ether, chloroform, ethanol and aqueous extract of the plants contains a good number of phytochemicals such as flavonoid, cardiac glycoside, saponin, alkaloid etc. as well as antioxidant with respect to the scavenging activity against free radicals DPPH which were roughly comparable to that of BHT. The plant extracts also exhibited potent antimicrobial activity against S. aureus, P. aeruginosa, S. mutans, E. coli and A. niger indicating its bactericidal and fungicidal properties. It may be concluded that the plant Nervilia crociformis is chemically more active than Nervilia...
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Posted by admin on Nov 30, 2018 in |
Microsphere drug delivery systems have used to improve patient compliance, decreases toxicity, and increase efficacy. Also, the use of microspheres to deliver drugs has many other merits, like control-release of the drug, increase bioavailability and target delivery of the drug to the desired site. In this research shows the utility of encapsulating hydroxypropyl methyl cellulose (Methocel K4M) in biodegradable microsphere delivery system, to be delivered orally via a capsule, to gives desired therapeutic action. Microsphere formulations have advantage over conventional tablet or capsule formulations, since it increases the surface area exposed to the absorption site and thus increasing the absorption of the drug and decreasing the dosing frequency of the drug. Flurbiprofen is a non-steroidal anti-inflammatory agent which is used widely in various colon diseases ulcerative colitis, Crohn’s disease, carcinomas and infections. Flurbiprofen shows maximum absorption in the lower gastrointestinal tract regions, and shows half life 4 – 5 h, it shows low bioavailability orally. The microsphere formulations were evaluated for its production yields, actual drug content, encapsulation efficiency,...
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Posted by admin on Nov 30, 2018 in |
Tecoma stans is a well-known traditional plant in India. The plant can be used in diabetes, digestive problems, and rheumatism and as antimicrobial. The aim of the study is to evaluate the anti-inflammatory activity of extracts of the stem bark of Tecoma stans (L.) by in-vitro and in-vivo methods. Ethyl acetate and alcoholic extract showed better anti-inflammatory activity by Human red blood cell membrane stabilization method. These extracts were also found to have significant inhibition to the carrageenan induced rat paw oedema. The gallic acid, a phenolic acid identified in the ethyl acetate fraction may contribute to the anti-inflammatory activity. The amount of gallic acid present in the ethyl acetate fraction of Tecoma stans (L.) was found to be 1.021 ng/mg extract, by HPTLC. The study concludes that the anti-inflammatory activity of stem bark of Tecoma stans may be due to the phenolics present in the extracts. Further studies are suggested to isolate and identify the more components responsible for the...
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Posted by admin on Nov 30, 2018 in |
Objective: The purpose of the study was to evaluate the effect of an Ayurvedic formulation Varanadi kashayam to inhibit pancreatic lipase activity in-vitro. It also focuses on antioxidant activity, total phenol, flavonoid and phenolic acid content of fractions of Varanadi kashayam and HPTLC fingerprinting of fractions. Methods: Fractionation of Varanadi kashayam was done by using five solvents of increasing polarity. Qualitative analysis of phyto-chemicals and quantitative determination of phenol, flavonoids, and phenolic acids were determined by standard protocols. The antioxidant activity of fractions was measured by DPPH and ABTS scavenging assay through spectroscopic analysis. The anti-lipase activity of fractions of Varanadi kashayam was determined by using porcine pancreatic lipase as enzyme and p-Nitrophenyl palmitate as substrate. HPTLC analytical method was used for the determination of compounds presents in polyherbal formulation Varanadi kashayam. Results: Ethyl acetate fraction of Varanadi kashayam showed free radical scavenging activity with IC50 values close to that of BHT and quercetin standards. The amount of phenol, flavonoid, and phenolic acid was also found to be highest...
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Posted by admin on Nov 30, 2018 in |
The application of medicinal plants such as Terminalia arjuna to maintain health and treat disease started since time immemorial and still is a part of medical practice. The phytochemical analysis of bark of T. arjuna in methanolic, hydromethanolic (1:1), aqueous and petroleum ether extracts revealed the presence of carbohydrates, amino acid, alkaloids, saponins, tannins, flavonoids, terpenoids, glycosides, xanthoproteins and phenols. In-vitro antioxidant capacity by linear regression analysis was measured by assaying DPPH radical and H2O2 scavenging capacities. Their respective IC50 values were found to be 336.89 μg/ml and 253.01 μg/ml. The hydromethanolic extract gave total phenolic, total flavonoids and FRAP values of 754.16 ± 16.26 mg GAE/g, 247.02 ± 8.81 mg/g QE/g and 1.36 ± 0.03 mM FeSO4 respectively. The IC50 values for in-vitro anti-inflammatory activities were evaluated by the following assays: lipoxygenase inhibition (IC50 = 480.5 μg/ml), heat induced protein denaturation (IC50 = 282.32 μg/ml), proteinase inhibition (IC50 = 1339.3 μg/ml) and RBC membrane stabilization (IC50 = 192.81 μg/ml) at different concentrations using aspirin as control. Studies were...
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