Posted by admin on Jan 31, 2019 in |
Objective: The main aim of the present study is to investigate the activity of the anti-osteoporotic activity of Cucurbita pepo seed powder (CPSP) and low level laser beam (LLLT) on glucocorticoid induced osteoporosis in rat model. Materials and Methods: Animals were randomly divided into six groups containing six rats each (n=6). Group I normal control, Group II dexamethasone induced osteoporotic control (7 mg/kg b.w i.m.), Group III received dexamethasone + LLLT irradiation (180 sec/day daily), Group IV received dexamethasone + CPSP (100 mg/kg b.w p.o), Group V received dexamethasone + sodium alendronate (3 mg/kg b.w p.o), Group VI dexamethasone + CPSP + LLLT. The treatment is made for 8 weeks. On the end of 4th and 8th week body weight were recorded, behavioral parameters, locomotar activity using actophotometer and anxiety levels using elevated plus maze (EPM) was studied. Urine parameters (calcium, phosphorous, creatinine), the blood was withdrawn from retro-orbital plexus and the serum was used for the estimation of ALP, calcium, phosphorous, creatinine. At the end of the 8th...
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Posted by admin on Jan 31, 2019 in |
The desired target Compounds (4a-e), (5a-e) and (6a-e) were prepared in two steps. Reaction of 3-acetyl-6-bromo-2H-chromen-2-one (1) with 3-(Aryl)-1-phenyl-1H-pyrazole-4-carbaldehydes (2a-e) in presence of aqueous KOH solution and the reaction mixture stirred for 2 hours and then filtered, furnished the desired 6-bromo-3-((E)-3-(3-(Aryl)-1-phenyl- 1H-pyrazole-4-yl) acryloyl)- 2H-chromen-2- ones (3a-e) crystalline solids in 60-70% yield (Scheme-1). Further, compounds (3a-e) reaction with thiourea, urea and hydrazine hydrate in the presence of a base in EtOH yielded the compounds (4a-e), (5a-e) and (6a-e) in 50-85% yield. All the synthesized compounds have been characterized by spectral data’s, and they are screened for anti-microbial activity. Among synthesized compounds 3b, 3c, 3d, 5a, and 5d exhibited good activity against bacterial strains S. aureus, P. aeruginosa, and E. coli, respectively. Whereas other compounds showed poor to moderate activity against all bacterial strains and compounds 3a, 3b, 3d, 4c, 5d, and 5c exhibited good antifungal activity against A. niger, and A. flavus and remaining compounds exhibited moderate antifungal activity. Results were tabulated in Table...
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Posted by admin on Jan 31, 2019 in |
The main objective of the present research was to develop mixed micelles using two biocompatible copolymers, D-α-tocopheryl polyethylene-glycol 1000 succinate (TPGS) and poloxamer 188 (P188) to improve the aqueous solubility and targeting efficacy of Simvastatin (SMV) against a variety of hormone-dependent cancers. A solvent evaporation technique prepared the plain/single copolymer micelles (SCMs) and mixed micelles (MMs). The prepared SCMs and MMs were characterized for critical micelle concentration (CMC), SMV content, particle size by dynamic light scattering (DLS), surface morphology by transmission electron microscopy (TEM), in-vitro SMV release and hemolysis. The SCMs and MMs showed mean particle size of 98 ± 5 nm and 129 ± 6 nm, respectively. SCMs showed SMV loading of 79.7 ± 5.6% while MMs exhibited improved SMV loading of 94.5 ± 6.5. The developed MMs system showed significantly lower CMC (3.5 fold less) than SCMs revealing their higher in-vivo stability. Moreover, SCMs and MMs exhibited zero order release profile, lower hemolytic behavior (<5% of hemolysis), when compared to plain SMV solution. The in-vitro cytotoxicity assay...
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Posted by admin on Jan 31, 2019 in |
A new derivative of Guar gum, incorporated with 1, 3, 4-thiadiazole nucleus was synthesized by using a new green and efficient synthetic approach. Guar gum is a biodegradable polymer having numerous applications in various industries. Thiadiazole compounds are well known in the therapeutic world due to their pharmacological importance. The newly synthesized derivative was characterized by IR, H1 NMR, and mass spectrometry. Its antioxidant activity was determined by hydrogen peroxide scavenging activity. Its antimicrobial activity was also...
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Posted by admin on Jan 31, 2019 in |
Analytical scientists of the pharmaceutical industry aimed at developing robust HPLC methods for analysis of generic drug products. This paper deals with HPLC method of analysis for estimation of % drug release of Ledipasvir and Sofosbuvir in Ledipasvir and Sofosbuvir tablets used for the treatment of chronic hepatitis C virus (HCV) infection. For analysis of dual combination drug product, HPLC method uses ‘Zorbax Eclipse Plus C18 100 × 4.6 mm, 3.5 µm’ HPLC column, a combination of buffer pH 3.0 and acetonitrile as mobile phase in gradient mode with UV detection at 260 nm. The method was validated and found to be precise, robust, accurate and linear (in range 9.05 to 54.3 µg/mL and 2.045 to 12.27 µg/mL of Sofosbuvir and Ledipasvir respectively). The method was also found to be specific for blank and placebo solutions. This ensures suitability of the method for simultaneous quantitative determination of % drug release of Ledipasvir and Sofosbuvir in a pharmaceutical formulation. In the pharmaceutical industry, this method can be used for routine...
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