Posted by admin on Jan 31, 2019 in |
Objective: To develop accurate, precise stability indicating a method for simultaneous estimation of Elbasvir and Grazoprevir in bulk and pharmaceutical dosage form. Materials and Methods: Simple, rapid, precise, sensitive and reproducible validated stability-indicating Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) method for the quantitative analysis of Elbasvir and Grazoprevir in the pharmaceutical dosage form. Chromatographic separation was carried out on waters Alliance-2695, by using Luna C18 (150 mm × 4.6 mm, 5 µm) column and the mobile phase containing OPA buffer (0.1%) and acetonitrile in the ratio of 50:50 v/v. The flow rate was 1.0 ml/min; detection was carried out at 258 nm using a photodiode array detector at ambient temperature. Results: The number of theoretical plates and tailing factor for Elbasvir and Grazoprevir were obtained to be NLT 2000 and should not more than 2 respectively. The linearity of the method was excellent over the concentration range 1.53-22.95 µg/ml and 3.05-45.75 µg/ml for Elbasvir and Grazoprevir respectively. The correlation coefficient was 0.999%. The relative standard deviation of peak areas of...
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Posted by admin on Jan 31, 2019 in |
Introduction: Autism spectrum disorders (ASD) are a group of mental problems that interfere with social interaction skills, and behavioral spectrum. ASDs are one of the most prevalent developmental disorders among children. The purpose of this study was to examine the mental health, quality of life, and happiness in mothers who had ASDs children and compare them with mothers having healthy children. Materials and Methods: This is a descriptive-analytic study. The population consisted of all mothers of children with autistic spectrum disorders (ASD) in Guilan province. The samples were determined through convenience sampling. We included 90 mothers whose children were identified with autism according to DSM5 criteria based on the clinical diagnosis of the child and adolescent psychiatrist and 90 mothers of children with no psychological complaint. The two groups were matched regarding demographic characteristics. The mothers of both groups completed a demographic questionnaire, the Symptom Checklist-90-R (SCL-90-R), the SF-36 Quality of Life Questionnaire, and the Oxford Happiness Questionnaire. The results were analyzed using descriptive statistics (i.e., mean, standard deviation)...
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Posted by admin on Jan 31, 2019 in |
Standardization is a process involving various chemical analysis methods based on pharmacological data involving general physical and microbiological analyzes aimed at the safety of a natural extract. To obtain the correct result, the standardization step is preceded by method validation. Quercetin can be obtained from the leaves of soursop (Annona muricata Linn.), which use for treatment fever, diarrhea, anti-convulsive, antifungal, antimicrobial, gout, itching, and flu. Determination of quercetin in soursop leaf extract using TLC-Densitometry must be validated by testing selectivity, limit of detection (LOD), limit of quantification (LOQ), linearity, precision, accuracy, and range. Densitometry scanning of the plates silica gel 60 F254 directly at 265 nm was used for analysis of quercetin. The result of selectivity test was 1.53 (>1.5) with mobile phase toluene: ethyl acetate: formic acid (4: 3: 0.4; v/v/v), limit of detection (LOD = 0.018 µg), limit of quantification (LOQ = 0.060 µg), linearity y = 9387.3x – 767.39 (r = 0.9996 and Vxo = 1.9%), Coefficient of Variation (C.V.) of precision 1.906525% and the percent...
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Posted by admin on Jan 31, 2019 in |
Hypercholesterolemia is one of the major conditions that are prevalent in today’s world, the significant causes being unhealthy food habits and lifestyle or abnormal genetic conditions. HMG CoA reductase is an important rate-limiting enzyme in the mevalonate pathway that synthesizes cholesterol. Biosynthesis of cholesterol in the liver can be reduced significantly by inhibiting HMG CoA reductase. Statins are the drugs used for the treatment of hypercholesterolemia. Due to the undesirable effects caused by synthetic statins, there is a need for the development of natural HMG CoA reductase inhibitors that are of plant origin. This study aimed to investigate the anti-inflammatory and HMG CoA reductase inhibitory effects of different hydro alcoholic plant extracts on the enzyme HMG CoA reductase activity obtained from Gallus gallus domesticus liver. Our study showed that the hydroalcoholic extract of Rosa centifolia exhibited the maximum inhibition of the enzyme under analysis with a ratio of 1.4117 and hence was subjected to solvent-solvent partition for the partial isolation of the inhibitory compounds. The result of this study...
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Posted by admin on Jan 31, 2019 in |
Preformulation testing is the first step in the rational development of dosage forms of a drug substance. The preformulation investigations confirm that there are no significant barriers to the compound’s development as a marketed drug. The formulation scientist uses this information to develop an efficacious, stable and safe dosage form. The main objective of the present research work was to do preformulation study of piroxicam drug. Piroxicam (PC) is one of the most potent non-steroidal anti-inflammatory agents that also has anti-pyretic activity and has been used for the treatment of rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, tendinitis, bursitis, and for the pain that is not related to a musculoskeletal system and traumatic contusions. The Principal advantage of piroxicam is its long half-life, which permits the administration of a single daily dose. As the piroxicam is very effective drug, many researchers use this drug in their research. This research paper helps those people who want to use piroxicam drug for their...
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