Posted by admin on Nov 30, 2018 in |
Antiviral properties of methanol and aqueous extracts from Morchella conica and Fomes fomentarius were assessed by colorimetric XTT test against human respiratory syncytial virus (HRSV). HEp-2 cell line was used for the cytotoxic and antiviral effects of the extracts. Non-cytotoxic concentrations of methanol and aqueous extracts of M. conica were tested in 50000 to 195 µg/mL. In contrast, the cytotoxic F. fomentarius methanol and aqueous extracts and Ribavirin were tested at concentrations from 3347-13.1 μg/mL, 4906.5-19.2 μg/mL and 86.60-0.30 μg/mL, respectively. The results showed that aqueous extract of F. fomentarius had strong anti-HRSV activity (EC50 = 358.7 μg/mL, SI = 27.4) which could be compared with ribavirin (EC50 = 15.6 μg/mL, SI = 11.1) used as a positive control against HRSV, while other extracts (M. conica methanol extract EC50 = 28055 μg/mL, SI = >1.8; F. fomentarius methanol extract EC50 = 3000 μg/mL, SI = 2.2) were found to have weak antiviral activity. As a result, it can be said that aqueous extract of F. fomentarius is worthy of...
Read More
Posted by admin on Nov 30, 2018 in |
Thiazoles derivatives are an important class of heterocyclic compounds, reported to possess a wide spectrum of biological activities. Moreover, thiazole nucleus occupies a very important place in the field of antioxidant agents. The above observations prompted us to synthesize some novel thiazole derivatives with various substitutions at along with heterocyclic rings in the same framework for synergistic action. We here in report the synthesis, antioxidant screening & QSAR studies of the new title compounds. Concentrated research on N-((Substituted) benzylidene)-4-(4-chlorophenyl) thiazole-2-carbohydrazides GS-5i-(a-k) & were synthesized, screened for antioxidant activities & QSAR studies. All new entities have good yield and results. From antioxidant activity results, it was observed that the compounds with both electron donating and electron withdrawing groups on the aldehydic phenyl ring influenced the activity. Among all the compounds tested GS-5i-b, GS-5i-d, GS-5i-e, GS-5i-h & GS-5i-i showed the good % inhibition and were found to be more significant compound among all the compounds tested. Compounds GS-5i-j were showed moderate % inhibition and were found to be significant among all...
Read More
Posted by admin on Nov 30, 2018 in |
The hydro dynamically balanced gastro retentive systems of Pregabalin has been formulated and evaluated which can retard the release of drug based on Super Porous Hydrogel Composites (SPHC) thereby prolong the release rate specifically in the upper part of the GIT. Fast swelling highly porous SPHCs are synthesized by gas blowing technique. The swelling property, mechanical strength and release profile of SPHCs containing drug was investigated by changing the amount of cross-linking agents such as N,N1-methylene-bis acrylamide (BIS) and Ac-Di-Sol. SPHCs to powder form was prepared, the obtained powder material used as matrix agent for the preparation of tablets. Floating tablets were prepared by wet granulation, novel melt granulation and direct compression methods using various grades of ethyl cellulose, HPMCK 100, eudragit ESPO, geleol, compritol 888 ATO and SPHC powder in different concentrations. The prepared gastro retentive floating tablets were evaluated in-vitro buoyancy studies, in–vitro and in-vivo release studies. Formulations consisting powdered SPH Ac-Di-Sol composite as matrix agent was optimized. The floating time observed >12 h at pH 1.2....
Read More
Posted by admin on Nov 30, 2018 in |
Lamotrigine (LTG), a synthetic anticonvulsant, may lead to therapy failure or toxicity risks due to the wide inter and intra individual pharmacokinetic variations. Those wide variations in LTG require its therapeutic drug monitoring (TDM). Our study aims to compare the QMS method with the HPLC-UV which is considered as a reference method for TDM of LTG. The study was conducted in the Clinical Pharmacology Department of the National Centre of Pharmacovigilance during a period of nine months. It included 37 samples from 37 patients treated with LTG. The samples were monitored by HPLC-UV and by PETIA. Regression analysis performed with data yielded to the equation LTGPETIA = 0.7701 LTGHPLC + 1.475 with a Pearson correlation r = 0.804 (P<0.0001). Bias mean between the two methods was 13.45%. In conclusion, the turbidometric technique (QMS) is comparable to the HPLC-UV method in the interval of therapeutic concentration. Under low concentrations, the QMS technique is less...
Read More
Posted by admin on Nov 30, 2018 in |
Antimicrobial activity of 11 ethnomedicinal plant extracts was evaluated against five bacterial strains (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Proteus vulgaris) and one fungal strain (Candida albicans). Plants were collected from different regions of Kashmir Valley and the ethnomedicinal data were gathered from traditional healers and elders of the study area. Cold percolation method was used to obtain hexane and methanol extracts and the antimicrobial activity was found using paper disc diffusion method. The results indicated that all plants, exhibited antimicrobial activity against one or more of the tested microorganisms at three different concentrations of 1.25, 2.5 and 5 mg/disc. Among the plants tested, Nepeta cataria, Allium consangium, Artemisia biensis and Ocimum sanctum were most active. The highest antifungal activity was exhibited by hexane extract of Nepeta catariaagainst Candida albicans. Compared to hexane extract, methanol extract showed significant activity against tested organisms. This study also showed that Nepeta cataria, Allium consangium, Artemisia biensis and Ocimum sanctum could be potential sources of new antimicrobial agents. Among the tested...
Read More
