Posted by admin on Dec 31, 2018 in |
Gastro-retentive drug delivery system can retain the drug in the gastric region for several hours which enhances drug release and improves bioavailability. Atenolol, an antihypertensive drug has a short half-life, limited bioavailability, highly unstable at basic pH and is extensively absorbed from the stomach. These features triggered the need for developing the gastro-retentive system. In the present study, a superporous hydrogel was developed as a gastro-retentive drug delivery system. Chitosan/poly (vinyl alcohol) interpenetrating polymer network (IPN) type superporous hydrogels were prepared using a gas foaming method employing glyoxal as the cross-linking agent. Sodium bicarbonate was used as a foaming agent to introduce the porous structure. Swelling behaviors of the superporous hydrogel in acidic solution were studied to investigate their applications for gastric retention device. The optimum preparation condition of superporous hydrogels was obtained from the gelation kinetics. SPH tablets of atenolol were prepared by direct compression incorporating microcrystalline cellulose, magnesium stearate, talc and sodium bicarbonate, etc. and evaluated. The super porous hydrogels were highly sensitive to pH of swelling...
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Posted by admin on Dec 31, 2018 in |
A reliable, rapid, simple and accurate high-performance liquid chromatography method with UV detection was developed for the simultaneous quantitative determination of strychnine in a polyherbal formulation prepared from Nux Vomica. Krimimudgara rasa, a herbal dosage form containing Strychnos nux vomica, Apium graveolens, Embellia ribes, Butea monosperma in combination. Separation of the strychnine from its major and minor degradation products was successfully achieved on a reversed-phase C-18 column using (250 mm × 4.6 mm ID, 5 µm particle size), with isocratic elution using a mixture of Methanol: KH2PO4 buffer solution (10 mmol, adjusted to pH 3 with orthophosphoric acid) (50:50 v/v) at flow rate 0.7 ml/min at 254 nm. The method was validated concerning linearity, precision, accuracy, system suitability and robustness. The responses were linear in the drug concentration range of 1 – 10 μg/ml. The percent recoveries were in the range between 98 – 101% from a mixture of degradation products. The utility of the procedure was verified by its application to a marketed formulation that was subjected to...
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Posted by admin on Dec 31, 2018 in |
Overcoming the scourge of malaria infection is possible when healthcare workers have good levels of understanding and attitudes towards the disease, which will influence their treatment practices. This study assessed healthcare workers’ levels of knowledge, attitudes, and practices (KAP) in uncomplicated malaria treatment at primary healthcare (PHC) facilities in Plateau state, Nigeria. The study employed a cross-sectional KAP approach to extract related data from 289 trained healthcare workers in twenty-four (24) PHC facilities. A designed and validated self-reported instruments known as Healthcare Knowledge, Attitude and Practice Instrument for Uncomplicated Malaria (HKAPIUM) (Cronbach’s alpha reliability = 0.705) were filled and returned by respondents, and the descriptive statistics of the data were analyzed using IBM Statistical Package for Social Sciences (SPSS®) version 23 software. Most respondents were within 38 and 47 (37.0%), and 28 – 37 (28.0%) years of age, and the majority (73.4%) were female. Many of them (31.8%) were as community health extension workers (CHEW), with only 11.4% and 0.7% as laboratory and pharmacy technicians, respectively. Those with 8...
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Posted by admin on Dec 31, 2018 in |
Pharmaceutical industry and quality control laboratories needs of robust methods for analysis of drugs used in the treatment of life-threatening diseases such as hepatitis C. Using HPLC technique, a rapid, selective, precise and accurate method was developed and validated for the estimation of % drug release of Sofosbuvir in a pharmaceutical formulation. Stability indicating HPLC method was developed using Zorbax eclipse plus C18 (100 × 4.6 mm), 3.5 µ as analytical column and a combination of ammonium acetate buffer pH 5.3 and methanol in the ratio (45: 55) was used as mobile phase in isocratic mode. UV detection was carried out at 260 nm, column temperature was maintained at 25 °C, and the flow rate was 1.5 ml/min. The method was validated as per internationally accepted ICH guideline and found to be specific for blank and placebo solution, precise, robust, accurate and linear in range 9.2 to 69.0 µg/ml of Sofosbuvir. This method can be used for routine analysis of pharmaceutical formulation in any quality control laboratory leading to...
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Posted by admin on Dec 31, 2018 in |
Three different species of Eupatorium namely E. glandulosum, E. odoratum and E. triplinerve belongs to the family Asteraceae are selected for the analysis of quercetin content. Leaves are extracted with ethanol and water and used for the analysis of quercetin by HPTLC technique using the mobile phase containing toluene: ethyl acetate: formic acid: methanol (5.5:4:1:0.5). Determination of quercetin content was performed by densitometric scanning under 254 nm, and the quercetin was detected at the Rf value of 0.54. The quantity of the quercetin content in plant extract was estimated by the calibration curve obtained from the standard quercetin. The result showed that the ethanolic extracts of E. glandulosum showed a high amount of quercetin (17.44 mg/g) followed by E. odoratum (13.4 mg/g) and E. triplinrve (9.29...
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