Posted by admin on Oct 31, 2018 in |
The aim of this study was to develop microspheres based orodispersible tablets of roxithromycin using the solvent evaporation technique. The microspheres were formulated in to orodispersible tablets using direct compression technique by central composite design. The objective of the study was to analyze the effect of concentration of eudragit RS100 (X1) and concentration of polyvinyl alcohol (X2), on the entrapment efficiency of the drug. Tablets were evaluated for physical characteristics viz. weight variation, hardness, friability, disintegration time and in-vitro dissolution study. The hardness and weight variation of the tablet were found 4.7 ± 0.24 kg/cm2 and 346.74 ± 3.74 mg, respectively. Friability of tablets was found 0.54 ± 0.04 which is less than 1% or in the acceptable limit. Disintegration time and drug release were found 45 ± 0.51 seconds and 96.45 ± 2.24% in one hour, respectively. The drug release data were subjected to drug release kinetics...
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Posted by admin on Oct 31, 2018 in |
Bisphenol-A (BPA) is a chemical produced in large volumes worldwide and has large market diffusion in many consumer products. BPA is used mainly as a monomer in the production of polycarbonate plastics and as a precursor of epoxy resins. So the present study was undertaken to examine the toxic effect of BPA on heart function in pregnant rat models. BPA (50 mg/kg.b.wt/day and 500 mg/kg.b.wt/day) was administered for 8th to 15th day by oral-gavage. The rats were sacrificed by cervical dislocation after last treatment. Paraffin embedded heart tissue sections were stained with hematoxylin-eosin. Significant degenerative changes were observed in H&E stained heart tissue sections of test rats. The activities of antioxidant enzymes like- superoxide dismutase, catalase and glutathione peroxidase were seen to be decreased significantly in treated rats; while, the level of malondialdehyde, a biomarker of lipid peroxidation, was increased than treated rats. Up-regulation of proapoptotic protein has been reported in heart failure and myocardial infarction, to determine whether caspase-3 protein can affect cardiac function in treated pregnant rats....
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Posted by admin on Oct 31, 2018 in |
Here we have carried out the acute and repeated dose toxicity study on tubers of Pueraria tuberosa water extract (PTWE). For acute toxicity study, rats were orally administered a single dose of 2000 and 5000 mg/kg body weight of PTWE. For repeated dose toxicity study on rats in one set, the different doses were given daily up to 28 days to make general observation and mortality rate. In another set of experiment, group of different doses were sacrificed on 7, 14, 21 and 28 days to assess the biochemical and histological changes. The selected doses were 250, 500, 1000 and 2000 mg/kg b.w. To evaluate it, we have used OECD guidelines 425 and 407 respectively, in both acute and repeated oral toxicity. No adverse effect was observed in all the rats, up to 14 days after treatment with single doses of 2000 and 5000 mg/kg b.w. Slight increase in salivation, mild diarrhea and letharginess were observed on 7th day at dose of 500 and 1000mg/kg, but recovered on 14th...
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Posted by admin on Oct 31, 2018 in |
Surgery alters the homeostatic balance and defence mechanisms in body eliciting certain responses called as stress response. In addition certain peri-operative factors may also influence the degree of stress response reflecting surgical recovery. The present study is an attempt to assess surgical stress response and to determine the influence of peri-operative factors on it. This study investigates the effects of surgery on early non specific immune and endocrine responses. 100 subjects undergoing minor and major elective surgeries were studied. Blood samples were collected before, immediately after and 72 h after the surgery. Total WBC count, differential neutrophil count, random blood sugar and pulse rate were assessed. Pain (Allina scale) and Depression Anxiety Stress scale (DAS) were used for evaluating the relation to stress response. In addition we used Estimation of Physiological Ability and Surgical Stress (E-PASS) scoring system for predicting the risk of post operative complications by quantifying patients reserve and degree of surgical stress. In minor surgeries, there was no significant drop in total counts after surgery, whereas...
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Posted by admin on Oct 31, 2018 in |
Background: Obesity is a confronting global issue with excess accumulation of body fat to an extent that decreases life expectancy. It is multifactorial in origin; genetic, environmental and pathophysiological. It leads to metabolic syndrome characterized by type 2 diabetes, cardiovascular diseases, fatty liver, hyperlipidemia etc. Objective: In this study, we have screened hydroalcoholic extracts of 7 plants for anti-obesity efficacy using inhibitory effect on lipid and carbohydrate metabolizing enzymes as indicators and further screening of these plants in 3T3 L1 cell line for inhibition of adipogenesis. Results: Among the 7 plants screened, Cyperus rotundus and Citrus limon showed highest inhibitory activity compared to other plants. These plants also showed antioxidant activity. Furthermore, these plants were investigated for their inhibition of adipogenesis and promotion of lipolysis in 3T3-L1cells. Cyperus rotundus and Citrus limon at 125 µg/mL showed inhibition of triglyceride accumulation and promoted lipolysis. Conclusion: Over all preliminary in-vitro screening study reveals Cyperus rotundus and Citrus limon showed potent anti obesity efficacy thus these plants may act as alternative herbal...
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